CAREER: Understand effector biology in the species complex Fusarium oxysporum

职业：了解尖孢镰刀菌物种复合体中的效应子生物学

• 批准号: 1652641
• 负责人: Li-Jun Ma
• 金额: $ 79.32万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-03-15 至 2023-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1652641&HistoricalAwards=false
• 关键词: CAREER; Understand; effector; biology; species

Pathogenic fungi pose serious threats to agricultural productivity. Fusarium oxysporum, an ascomycete fungus, is known to cause vascular wilt in over 100 plant species, including tomato, cotton, watermelon and banana, resulting in billions of dollars annual yield loss. The Ma lab's long-term goal is to understand the molecular mechanisms underlying Fusarium pathogenesis and plant defense for the development of disease resistant crops. This CAREER project will study both pathogenesis of F. oxysporum and plant defense using an established F. oxysporum - Arabidopsis pathosystem. A deeper understanding of this important pathogen will provide novel insights into interactions between soil-borne fungi and plant hosts. The advanced knowledge of plants defense against fungal pathogens will facilitate the development of durable disease-resistant crops. Therefore the research has the potential to transform world-wide disease management of many important crops that suffer from Fusarium wilts. Using the data and hypotheses generated from this research program, the PI will develop a Course-Based Undergraduate Research Experience (CURE) as part of the undergraduate curriculum at both UMass Amherst and Dominican College, a teaching intense college, whose students are 40% first generation college students, 66% women and over 40% minority students. Student participation will also bring fresh minds into the proposed research, increase the reproducibility and advance science. Fungal effectors manipulate host immunity and contribute significantly to the outcomes of fungal-plant relationships, ranging from pathogenic to mutualistic. This CAREER project will study F. oxysporum effector biology taking diverse approaches that include computational biology, reverse genetics, quantitative biophysics and live image analysis. Specifically, this project will first characterize function of 300 candidate effectors using reverse genetics (Aim 1). Then, functionally important effectors will be monitored to trace effector trafficking using quantitative biophysics and live cell imaging (Aim 2). Finally the project will dissect effector regulation through network modeling (Aim 3). Following a step-wise approach, this project will funnel through a large dataset and finally focus on a few important effectors. Built on an interdisciplinary collaboration, the project will introduce quantitative biophysics into the investigation of fungal effector biology. This approach will provide unprecedented, quantitative and transparent information at the cellular and molecular level on the interactions between fungal effectors and their host plant cells. A major Educational Objective of this CAREER project is to create an undergraduate course in which students test hypotheses generated in Aim 3 and bring authentic research into undergraduate education by integrating ChIPseq into our undergraduate curriculum.

病原真菌对农业生产构成严重威胁。尖孢镰刀菌是一种子囊菌，在番茄、棉花、西瓜和香蕉等100多种植物上引起维管萎萎病，每年造成数十亿美元的产量损失。MA实验室的长期目标是了解镰刀菌致病和植物防御的分子机制，以开发抗病作物。这个职业项目将研究尖孢镰刀菌的致病机理和利用已建立的尖孢镰刀菌-拟南芥病理系统进行植物防御。对这一重要病原菌的深入了解将为土传真菌与植物寄主之间的相互作用提供新的见解。植物抵御真菌病原菌的先进知识将促进耐久抗病作物的发展。因此，这项研究有可能改变世界范围内许多遭受枯萎病困扰的重要作物的疾病管理。利用这项研究项目产生的数据和假设，PI将开发基于课程的本科生研究体验(CURE)，作为马萨诸塞州大学阿默斯特分校和多米尼加学院本科课程的一部分，这是一所教学密集的大学，其学生中40%是第一代大学生，66%是女性，40%以上是少数族裔学生。学生的参与还将为拟议的研究带来新的思维，增加重复性和推进科学。真菌效应器操纵宿主免疫，对真菌-植物关系的结果做出重大贡献，从致病到互惠。这一职业项目将采用包括计算生物学、反向遗传学、定量生物物理学和实时图像分析在内的不同方法来研究尖孢镰刀菌效应器生物学。具体地说，该项目将首先使用反向遗传学来表征300个候选效应器的功能(目标1)。然后，将监测具有重要功能的效应器，以使用定量生物物理学和活细胞成像追踪效应器的运输(目标2)。最后，该项目将通过网络建模来剖析效应器的规则(目标3)。按照循序渐进的方法，这个项目将通过一个大型数据集进行漏斗，最后集中在几个重要的效应器上。该项目建立在跨学科合作的基础上，将把定量生物物理学引入真菌效应生物学的研究中。这种方法将在细胞和分子水平上提供前所未有的、定量和透明的信息，了解真菌效应器与寄主植物细胞之间的相互作用。这一职业项目的一个主要教育目标是创建一门本科课程，学生在其中测试目标3中产生的假设，并通过将CHIPSEQ整合到我们的本科课程中，将真实的研究带入本科教育。

项目成果

Differential Colonization of the Plant Vasculature Between Endophytic Versus Pathogenic Fusarium oxysporum Strains

内生与致病性尖孢镰刀菌菌株之间植物维管系统的差异定殖

• DOI: 10.1094/mpmi-08-22-0166-sc
• 发表时间: 2023
• 期刊: Molecular Plant-Microbe Interactions®
• 作者: Martínez-Soto, Domingo;Yu, Houlin;Allen, Kelly S.;Ma, Li-Jun
• 通讯作者: Ma, Li-Jun

Improved Assembly of Reference Genome Fusarium oxysporum f. sp. lycopersici Strain Fol4287

• DOI: 10.1128/mra.00910-18
• 发表时间: 2018-09-01
• 期刊: MICROBIOLOGY RESOURCE ANNOUNCEMENTS
• 影响因子: 0.8
• 作者: Ayhan, Dilay Hazal;Lopez-Diaz, Cristina;Ma, Li-Jun
• 通讯作者: Ma, Li-Jun

Genome Sequence of Fusarium oxysporum f. sp. matthiolae , a Brassicaceae Pathogen

尖孢镰刀菌 f 的基因组序列。

• DOI: 10.1094/mpmi-11-19-0324-a
• 发表时间: 2020
• 期刊: Molecular Plant-Microbe Interactions®
• 作者: Yu, Houlin;Ayhan, Dilay Hazal;Diener, Andrew C.;Ma, Li-Jun
• 通讯作者: Ma, Li-Jun

The genome of opportunistic fungal pathogen Fusarium oxysporum carries a unique set of lineage-specific chromosomes

• DOI: 10.1038/s42003-020-0770-2
• 发表时间: 2020-01-31
• 期刊: COMMUNICATIONS BIOLOGY
• 影响因子: 5.9
• 作者: Zhang, Yong;Yang, He;Ma, Li-Jun
• 通讯作者: Ma, Li-Jun

Effector Gene Suites in Some Soil Isolates of Fusarium oxysporum Are Not Sufficient Predictors of Vascular Wilt in Tomato

• DOI: 10.1094/phyto-12-16-0437-r
• 发表时间: 2017-07-01
• 期刊: PHYTOPATHOLOGY
• 影响因子: 3.2
• 作者: Jelinski, Nicolas A.;Broz, Karen;Kistler, H. Corby
• 通讯作者: Kistler, H. Corby