CAREER: Integration of photonic crystals and paper-based microfluidics for biosensing

职业：光子晶体和纸基微流体的集成用于生物传感

• 批准号: 1653673
• 负责人: Meng Lu
• 金额: $ 50万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-03-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1653673&HistoricalAwards=false
• 关键词: CAREER; Integration; photonic; crystals; paper

Despite significant advances in diagnostic technologies, new tests are strongly demanded to identify a specific biological signature with high sensitivity and also provide quantitative information. Protein biomarkers circulating in human body fluids have a great potential to be used in the clinic to indicate diseases. To date, the poor sensitivity and high cost are still the key challenges prohibiting a practical point-of-care diagnostic test based on quantifying low-abundance disease biomarkers. In this project, the investigator aims to establish a low-cost, high sensitivity, multiplexed, rapid, and automated paradigm based upon a hybrid sensor in conjunction with a new readout modality that can perform biomarker analysis for disease diagnostics. The project focuses on investigating the paper-based nanophotonics that integrates photonic crystal devices and paper-based microfluidics, and on how to exploit the unique optical phenomena enabled by the photonic crystal for the biomarker detection with high sensitivity and specificity. In particular, the inexpensive integration of nanophotonic devices with paper substrates that can filter and transport body fluid samples is being addressed. The low-cost and disposable feature of the sensor is highly desirable in resource-limited settings, such as bedside test, remote military deployment, and developing countries. In addition, the sensor is ready to be modified for the detection of pathogenic species that causes server diseases, such as hepatitis, tuberculosis, and HIV. The project also includes education and outreach activities to foster undergraduate students' passion for research, engage underrepresented minority students, and bring science and technology to K-12 schools and agriculture communities in the Midwest.Rapid and accurate diagnostics is fundamental to healthcare, regardless of whether it is used for an individual patient with a disease, such as cancer, or for a worldwide epidemic. The research goal of this project is to establish a low-cost, high sensitivity, multiplexed, rapid, and automated biosensor based upon a hybrid device and a new readout modality that can perform biomarker analysis for clinical diagnostics. The transformative aspect of this project is to provide fundamental insights and evidence into the paper-photonic crystal hybrid system, which would enable the invention of new disposable. The novelty of the proposed works lies in two facts: i) the integration of photonic crystal and paper as a flow-through sensor platform and ii) the application of a photoacoustics-based readout scheme. In particular, the project will be carried out by combining numerical models and experiments according to the following three specific research objectives: i) integration of photonic crystal and paper-based microfluidics, ii) establishment of photonic crystal-enhanced photoacoustic detection for paper-based biosensor, and iii) multiplexed detection for in-vitro disease diagnostics. The project outcome will fill a significant gap between optofluidics and point-of-care tests by addressing challenging questions including: What are the fluid transport phenomena in a photonic crystal-paper hybrid system? How can photonic crystal structure enable ultrahigh sensitivity in paper-based biosensors? How can the photoacoustic detection improve the detection sensitivity and eliminate background noises from paper substrates?

尽管诊断技术取得了重大进展，但仍强烈需要新的检测方法，以高灵敏度识别特定的生物特征，并提供定量信息。在人体体液中循环的蛋白质生物标志物在临床上用于指示疾病具有很大的潜力。迄今为止，低灵敏度和高成本仍然是阻碍基于定量低丰度疾病生物标志物的实用床旁诊断测试的关键挑战。在这个项目中，研究人员的目标是建立一个低成本，高灵敏度，多路复用，快速和自动化的范例，基于一个混合传感器结合一个新的读出模式，可以执行生物标志物分析疾病诊断。该项目的重点是研究集成光子晶体器件和纸基微流体的纸基纳米光子学，以及如何利用光子晶体实现的独特光学现象进行高灵敏度和特异性的生物标志物检测。特别是，正在解决将纳米光子器件与可以过滤和运输体液样品的纸基底廉价集成的问题。传感器的低成本和一次性特征在资源有限的环境中是非常可取的，例如床边测试、远程军事部署和发展中国家。此外，该传感器还可以进行修改，用于检测导致严重疾病的病原体，如肝炎，结核病和艾滋病毒。该项目还包括教育和外展活动，以培养本科生对研究的热情，吸引代表性不足的少数民族学生，并将科学和技术带到中西部的K-12学校和农业社区。快速准确的诊断是医疗保健的基础，无论它是用于患有某种疾病（如癌症）的个体患者，还是用于全球流行病。该项目的研究目标是建立一种低成本，高灵敏度，多路复用，快速，自动化的生物传感器，基于混合装置和新的读出模式，可以进行生物标志物分析的临床诊断。该项目的变革方面是为纸-光子晶体混合系统提供基本的见解和证据，这将使新的一次性用品的发明成为可能。所提出的工作的新奇在于两个事实：i）作为流通传感器平台的光子晶体和纸的集成和ii）基于光声的读出方案的应用。特别是，该项目将根据以下三个具体研究目标通过结合数值模型和实验来进行：i）光子晶体和纸基微流体的集成，ii）建立用于纸基生物传感器的光子晶体增强光声检测，以及iii）用于体外疾病诊断的多路检测。该项目的成果将通过解决具有挑战性的问题填补光流体和即时检测之间的重大差距，包括：光子晶体-纸混合系统中的流体传输现象是什么？光子晶体结构如何使纸基生物传感器具有灵敏度？光声检测如何提高检测灵敏度和消除纸基的背景噪声？

项目成果

Imprint and transfer fabrication of freestanding plasmonic membranes

• DOI: 10.1088/1361-6528/ab98bf
• 发表时间: 2020-06
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Longju Liu;H. Monshat;Hsin-Yu Wu;Meng Lu

A Narrowband Photo‐Thermoelectric Detector Using Photonic Crystal

• DOI: 10.1002/adom.201801248
• 发表时间: 2018-12
• 期刊: Advanced Optical Materials
• 影响因子: 9
• 作者: H. Monshat;Longju Liu;Meng Lu

An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests

• DOI: 10.1016/j.bios.2020.112651
• 发表时间: 2020-12-01
• 期刊: BIOSENSORS & BIOELECTRONICS
• 影响因子: 12.6
• 作者: Liu, Mingdian;Zhao, Yuxin;Lu, Meng
• 通讯作者: Lu, Meng

Resonant chalcogenide-metal-fluoropolymer nanograting for tunable pyroelectric sensing

用于可调谐热释电传感的谐振硫属化物-金属-含氟聚合物纳米光栅

• DOI: 10.1364/cleo_at.2021.aw4m.7
• 发表时间: 2021
• 期刊: CLEO: Applications and Technology
• 作者: Wei, Le;Qian, Jingjing;Dong, Liang;Lu, Meng
• 通讯作者: Lu, Meng

Paper-based isothermal DNA amplification and real-time analysis

纸质等温 DNA 扩增和实时分析

• DOI: 10.1109/sensors43011.2019.8956819
• 发表时间: 2019
• 期刊: 2019 IEEE SENSORS
• 作者: Liu, Mingdian;Monshat, Hosein;Zhao, Yuxin;Lu, Meng
• 通讯作者: Lu, Meng