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Collaborative Research: Genome-Scale Phylogeographic Analysis of Desmognathus Salamanders

合作研究：蝾螈的基因组规模系统发育地理学分析

基本信息

批准号：
1656111
负责人：
David Beamer
金额：
$ 9.64万
依托单位：
Nash Community College
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2021-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656111&HistoricalAwards=false
关键词：
Collaborative Research Genome Scale Phylogeographic

项目摘要

The Dusky Salamanders, genus Desmognathus, are one of the most diverse and abundant animals in the eastern forests of the United States. They are classic study organisms for ecology and behavior. Along with frogs and other salamanders worldwide, they are also disappearing at an alarming rate, possibly due to factors such as habitat loss, infectious diseases, and other environmental stresses. Despite their visibility and imperilment, we know little about their genetic diversity and evolutionary history. While there are only 21 currently named species, recent work using DNA sequencing has revealed at least 45 potential species, which would more than double the size of the group. This research will reveal the true diversity of the group, providing a future platform for ecological and evolutionary studies of Desmognathus, and guiding conservation-management decisions for this imperiled group of aquatic vertebrates. Broader impacts will include a K-12 teaching module for understanding forest ecology, centered on salamanders, and updated species accounts for local wildlife-management authorities. This will be invaluable for understanding the biodiversity heritage of the United States.With an exhaustive "ecodrainage" sampling technique, this research will analyze 7,000 samples from nearly every known Desmognathus population. The study will use next-generation genomic sequencing of DNA to understand the true genetic diversity of the group. These data will allow researchers to understand the evolutionary history of divergence, hybridization, and species boundaries from a genetic perspective, using modern species-delimitation methods. Many species are also difficult to distinguish by eye. Thus, this research will complement the genetic perspective with 3-dimensional geometric morphometric analyses of size and shape, to reveal the subtle but significant morphological differences between populations. Overall, this research will generate a robust and fully-sampled estimate of the true number of Desmognathus species, their morphological variation for identifying them in the field, and their geographic range. This research will likely yield an increased number of Desmognathus species, while also showing a complex history of introgression, hybridization, and incomplete ecological speciation in the group.

Dusky salamander， Desmognathus属，是美国东部森林中最多样化和最丰富的动物之一。它们是生态学和行为学的经典研究对象。与青蛙和世界各地的其他蝾螈一样，它们也在以惊人的速度消失，可能是由于栖息地丧失、传染病和其他环境压力等因素。尽管它们很显眼，也很危险，但我们对它们的遗传多样性和进化历史知之甚少。虽然目前只有21个被命名的物种，但最近利用DNA测序的工作揭示了至少45个潜在物种，这将使该群体的规模增加一倍以上。该研究将揭示该类群的真正多样性，为未来的生态和进化研究提供平台，并指导这一濒危水生脊椎动物群体的保护管理决策。更广泛的影响将包括一个以蝾螈为中心的了解森林生态的K-12教学模块，以及为当地野生动物管理当局更新物种统计。这对于了解美国的生物多样性遗产将是无价的。通过详尽的“生态排水”采样技术，这项研究将分析几乎所有已知的地毛象种群的7000个样本。这项研究将使用下一代DNA基因组测序来了解该群体真正的遗传多样性。这些数据将使研究人员能够使用现代物种划分方法，从遗传角度了解分化、杂交和物种边界的进化史。许多物种也很难用眼睛区分。因此，本研究将以大小和形状的三维几何形态计量学分析来补充遗传学观点，以揭示种群之间细微但显著的形态差异。总的来说，这项研究将产生一个可靠的和全采样的假蝇种类的真实数量，它们在野外识别的形态变化，以及它们的地理范围的估计。这项研究可能会产生更多的Desmognathus物种，同时也显示了该群体的渗入、杂交和不完整的生态物种形成的复杂历史。