Engineering New REU Approaches to Challenges in MultiTERM

REU 设计新方法应对 MultiTERM 挑战

• 批准号: 1659663
• 负责人: Anthony Atala
• 金额: $ 36.64万
• 依托单位: Wake Forest University School of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1659663&HistoricalAwards=false
• 关键词: Engineering; New; REU; Approaches; Challenges

This three year Research Experiences for Undergraduates (REU) Site program, Engineering New REU Approaches to Challenges in MultiTERM at the Wake Forest Institute for Regenerative Medicine (WFIRM), will provide research opportunities at the interface of engineering and biology in challenging areas of tissue engineering and regenerative medicine (TERM) to a diverse cohort of undergraduate students from underrepresented groups with limited access to research facilities. The proposed research projects span multiple physiological systems (cardiovascular, musculoskeletal, urological, and endocrine), providing REU students a unique opportunity to become highly skilled problem solvers by appreciating generalized, system-independent approaches with many of the projects identified as high priority by NSF and DoD. Despite early successes, four main challenges remain spanning: 1) biomaterials, 2) cell sources, 3) vascularization and 4) enabling technologies such as cell delivery devices, 3D bioprinting and tissue bioreactors. REU projects will help address these challenges. WFIRM will host 10 undergraduate students over 10 summer weeks where they will engage in highly interdisciplinary and challenging areas of TERM research. Outcome objectives of the program emphasize: 1) development of interest and excitement for team-based-studies and advanced education; 2) skill transference and hands-on exposure to modern instrumentation and techniques as well as rigorous analysis; 3) enhanced verbal and written communication skills emphasizing abilities to communicate across disciplines in lay and technical terms and in formally presenting in writing (for the scientific literature) and speaking (for scientific meetings); and, ultimately, 4) to initiate socialization into the very important process of growth from dependent to independent researcher status. The REU experience is supplemented with additional program elements and education on the research process, hands-on instrumentation training, workshops on bioethics and responsible conduct of research, effective oral and written communications workshops, professional development and career opportunities topics. To increase program impact and sense of community, REU participants will also participate in non-research and outreach activities at WFIRM and with URM partners, providing forums to discuss implications of STEM advances to the larger society, while fostering networking with other students, engineers, scientists and educators. All students will present their research findings several times over the summer and in oral and poster format at a final research day event. Opportunities to attend and present at local and national meetings is also emphasized.

维克森林再生医学研究所(WFIRM)的这项为期三年的本科生研究经验(REU)现场项目--应对多重挑战的工程新REU方法--将在具有挑战性的组织工程和再生医学(TERM)领域为不同的本科生提供工程学和生物学相结合的研究机会，这些本科生来自缺乏代表性的群体，获得研究设施的机会有限。拟议的研究项目跨越多个生理系统(心血管、肌肉骨骼、泌尿系统和内分泌)，为REU学生提供了一个独特的机会，通过欣赏通用的、独立于系统的方法来成为高技能的问题解决者，其中许多项目被NSF和国防部确定为高度优先。尽管取得了早期的成功，但仍然存在四个主要挑战：1)生物材料，2)细胞来源，3)血管形成，4)使能技术，如细胞输送装置，3D生物打印和组织生物反应器。REU项目将有助于应对这些挑战。WFIRM将在10个暑假周接待10名本科生，在那里他们将从事高度跨学科和具有挑战性的学期研究领域。该计划的成果目标强调：1)培养对基于团队的研究和高级教育的兴趣和兴奋；2)技能转移和亲身接触现代仪器和技术以及严格的分析；3)提高口头和书面沟通技能，强调用非专业和技术术语进行跨学科交流的能力，以及以书面形式(为科学文献)和演讲(为科学会议)的能力；以及，最终，开始社会化，进入从依赖到独立研究人员地位的非常重要的成长过程。REU的经验还包括关于研究过程的其他计划要素和教育、动手仪器培训、关于生物伦理和负责任的研究行为的研讨会、有效的口头和书面交流研讨会、专业发展和职业机会主题。为了增加项目的影响力和社区意识，REU参与者还将参加WFIRM和URM合作伙伴的非研究和外联活动，提供论坛讨论STEM进步对更大社会的影响，同时促进与其他学生、工程师、科学家和教育工作者的联系。所有学生将在暑假期间多次展示他们的研究成果，并在最后的研究日活动中以口头和海报的形式展示。还强调了出席地方和国家会议并在会上发言的机会。

项目成果

Administration of FVIII-Expressing Human Placental Cells to Juvenile Sheep Yields Multi- Organ Engraftment, Therapeutic Plasma FVIII Levels and Alter Immune Signaling Pathways to Evade FVIII Inhibitor Induction

将表达 FVIII 的人胎盘细胞给予幼年绵羊可实现多器官移植、治疗性血浆 FVIII 水平并改变免疫信号传导途径以逃避 FVIII 抑制剂诱导

• 发表时间: 2021
• 期刊: ASH
• 作者: Trevisan, Brady;Rodriguez, Martin;Dizon, Jacqueline;George, Sunil;Shields, Jordan;Lankford, Shannon;Combs, Rebessa;Owen, John;Atala, Anthony;Doering, Christopher
• 通讯作者: Doering, Christopher

Human placental-derived stem cell therapy ameliorates experimental necrotizing enterocolitis

人胎盘干细胞疗法可改善实验性坏死性小肠结肠炎

• DOI: 10.1152/ajpgi.00369.2020
• 发表时间: 2021
• 期刊: American Journal of Physiology-Gastrointestinal and Liver Physiology
• 影响因子: 4.5
• 作者: Weis, Victoria G.;Deal, Anna C.;Mekkey, Gehad;Clouse, Cara;Gaffley, Michaela;Whitaker, Emily;Peeler, Cole B.;Weis, Jared A.;Schwartz, Marshall Z.;Atala, Anthony
• 通讯作者: Atala, Anthony

Using a Human Liver Tissue Equivalent (hLTE) Platform to Define the Functional Impact of Liver-Directed AAV Gene Therapy

使用人类肝脏组织等效物 (hLTE) 平台定义肝脏定向 AAV 基因治疗的功能影响

• 发表时间: 2021
• 期刊: ASH
• 作者: Ramamurthy, R;Zheng, WT;George, S;Wan, M;Zhou, Y;Bishop, C;Atala, A;Porada, C;Almeida-Porada, G
• 通讯作者: Almeida-Porada, G

Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction

• DOI: 10.1038/s41598-020-66487-8
• 发表时间: 2020-06-17
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Nzou, Goodwell;Wicks, Robert T.;Atala, Anthony J.
• 通讯作者: Atala, Anthony J.