Dissertation Research: Assessing the vulnerability of salt marsh carbon storage to nutrient enrichment using an integrated meta-omics framework

论文研究:使用综合元组学框架评估盐沼碳储存对养分富集的脆弱性

基本信息

  • 批准号:
    1701748
  • 负责人:
  • 金额:
    $ 1.99万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-06-01 至 2018-05-31
  • 项目状态:
    已结题

项目摘要

As human populations increase, so will the demand for food. Nitrogen fertilizer production helps meet this need. However, adding excess nitrogen drastically alters natural habitats such as salt marshes and others that receive water as runoff from land. Coastal wetlands provide several ecosystem services, including nursery habitat for fish and birds, storm surge protection, and nutrient filtration. Prior studies suggest that excess nitrogen may increase decomposition in these sediments, resulting in faster degradation of complex organic matter to carbon dioxide. What controls these processes, and the micro-organisms responsible for them is not well understood. This doctoral dissertation improvement project will use controlled laboratory experiments to investigate the mechanisms behind the effect of added nitrogen on organic matter decomposition. This research will provide an in-depth analysis of the members of the salt marsh microbiome and their activities in marsh sediments. Data will provide insight into how salt marshes are responding to nitrogen fertilizer runoff, information that is essential for the adaptive management of coastal wetland systems and their response to environmental change. This doctoral dissertation improvement project will use controlled flow through reactor systems to investigate the mechanistic effect of nitrate on microbial decomposition of salt marsh sediment organic matter. We hypothesize that, because nitrate is a favorable electron acceptor in heterotrophic, microbial respiration, it can promote oxidation of organic matter that would otherwise remain unprocessed. Current biogeochemical data indicate a significant increase in microbial respiration, particularly denitrification, in response to nitrate addition, with the most pronounced effect in deep sediments ranging from 150 to 200 years old. This is significant, because organic matter at this depth is typically thought to be more resistant to microbial degradation, which means that the capacity of salt marshes to store carbon may lessen with eutrophication. To further assess these results, we need to gain a better understanding of the microbial consortia responsible for organic matter decomposition. Microbial activity plays a critical role in controlling how much carbon is decomposed; though we know little about salt marsh sediment microbiome structure and function, and how it may change in response to nitrate addition. Accordingly, this dissertation improvement grant will contribute to existing data by taking an integrated 'meta-omics' approach. To complement flow through reactor experiments, the microbial genetic underpinnings of organic matter decomposition under conditions of nutrient enrichment will be investigated using metagenomics, and the metabolic activity of these microbial players will be assessed by measuring the expression of the microbial metatranscriptome. These data have the potential to reveal novel microbial lineages from deep salt marsh sediment, and draw unique linkages between microbial gene expression patterns and changes in organic carbon quality. Successful integration of these meta-omics approaches with existing geochemical rate measurements will vastly improve our understanding of the role of microbes in organic matter decomposition, and potentially allow for a better predictive understanding for how carbon storage capacity in salt marshes will respond to future nitrogen loading.
随着人口的增加,对食物的需求也会增加。氮肥生产有助于满足这一需求。 然而,添加过量的氮大大改变了自然栖息地,如作为盐沼泽和其他从陆地上接收水的地方。沿海湿地提供多种生态系统服务,包括鱼类和鸟类的育苗栖息地,风暴潮保护和营养过滤。先前的研究表明,过量的氮可能会增加这些沉积物的分解,导致复杂的有机物更快地降解为二氧化碳。控制这些过程的是什么,以及负责这些过程的微生物还没有得到很好的理解。本博士论文改进项目将使用受控实验室实验来研究添加氮对有机物分解的影响背后的机制。这项研究将深入分析盐沼微生物组的成员及其在沼泽沉积物中的活动。数据将提供深入了解盐沼是如何应对氮肥径流,信息是沿海湿地系统的适应性管理及其对环境变化的反应至关重要。本博士论文改良计画将利用控制流通过反应器系统来探讨硝酸盐对盐沼沉积物有机质微生物分解的机制效应。我们假设,因为硝酸盐是一个有利的电子受体在异养,微生物呼吸,它可以促进氧化的有机物,否则将保持未加工。目前的生物地球化学数据表明,微生物呼吸作用,特别是反硝化作用,显着增加,以应对硝酸盐的增加,在150至200岁的深层沉积物中的影响最显着。这一点很重要,因为在这个深度的有机物通常被认为更能抵抗微生物降解,这意味着盐沼储存碳的能力可能会随着富营养化而减弱。为了进一步评估这些结果,我们需要更好地了解负责有机物分解的微生物财团。微生物活性在控制分解多少碳方面起着关键作用;尽管我们对盐沼沉积物微生物组的结构和功能以及它如何响应硝酸盐的添加而变化知之甚少。因此,本论文改进补助金将通过采取综合的“元组学”方法来促进现有数据。为了补充通过反应器实验的流动,将使用宏基因组学研究在营养物富集条件下有机物分解的微生物遗传基础,并通过测量微生物元转录组的表达来评估这些微生物参与者的代谢活性。这些数据有可能揭示新的微生物谱系深盐沼沉积物,并绘制微生物基因表达模式和有机碳质量的变化之间的独特联系。这些元组学方法与现有的地球化学速率测量的成功整合将大大提高我们对微生物在有机物分解中的作用的理解,并可能更好地预测盐沼中的碳储存能力将如何响应未来的氮负荷。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Jennifer Bowen其他文献

A Preliminary Study of Klein Knots
克莱因结的初步研究
  • DOI:
  • 发表时间:
    2010
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Louisa Catalano;David Freund;Rutendo Ruzvidzo;Jennifer Bowen;John R. Ramsay
  • 通讯作者:
    John R. Ramsay
531: Preterm prelabor rupture of the membranes (PPROM) and childhood health and educational outcomes
  • DOI:
    10.1016/j.ajog.2016.11.266
  • 发表时间:
    2017-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    Jonathan Morris;Paul Wagland;Siranda Torvaldsen;Jason Bentley;Jennifer Bowen;Christine Roberts
  • 通讯作者:
    Christine Roberts
Centenarians and the genetics of longevity.
百岁老人和长寿遗传学。
  • DOI:
  • 发表时间:
    2000
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Thomas T Perls;Dellara F. Terry;M. Silver;Maureen Shea;Jennifer Bowen;Erin Joyce;Stephen B. Ridge;Ruth C. Fretts;Mark J. Daly;Stephanie J. Brewster;A. Puca;Louis M. Kunkel
  • 通讯作者:
    Louis M. Kunkel
Social justice and medical leadership: what is preventing trainees in the East Midlands from accessing leadership training?
社会正义和医疗领导力:是什么阻碍了东米德兰兹郡的学员接受领导力培训?
  • DOI:
    10.1136/leader-2023-000782
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    2.7
  • 作者:
    Rebecca Clegg;Jennifer Bowen
  • 通讯作者:
    Jennifer Bowen
Substrate oxidation in patients with cirrhosis: comparison with other nutritional markers.
肝硬化患者的底物氧化:与其他营养标志物的比较。

Jennifer Bowen的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Jennifer Bowen', 18)}}的其他基金

Collaborative Research: Quantifying the effects of different nitrogen forms on marsh resilience to environmental change
合作研究:量化不同氮形式对沼泽适应环境变化的能力的影响
  • 批准号:
    2203322
  • 财政年份:
    2022
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Continuing Grant
CAREER: Salt marsh restorations: a structured experiment for learning and teaching about salt marshes, microbial diversity, and ecosystem function
职业:盐沼恢复:用于学习和教学盐沼、微生物多样性和生态系统功能的结构化实验
  • 批准号:
    1719446
  • 财政年份:
    2016
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Continuing Grant
Collaborative Research: Ecosystem Evolution and Sustainability of Nutrient Enriched Coastal Saltmarshes
合作研究:营养丰富的沿海盐沼的生态系统演化和可持续性
  • 批准号:
    1719418
  • 财政年份:
    2016
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Continuing Grant
CAREER: Salt marsh restorations: a structured experiment for learning and teaching about salt marshes, microbial diversity, and ecosystem function
职业:盐沼恢复:用于学习和教学盐沼、微生物多样性和生态系统功能的结构化实验
  • 批准号:
    1350491
  • 财政年份:
    2014
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Continuing Grant
Collaborative Research: Ecosystem Evolution and Sustainability of Nutrient Enriched Coastal Saltmarshes
合作研究:营养丰富的沿海盐沼的生态系统演化和可持续性
  • 批准号:
    1353140
  • 财政年份:
    2014
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Continuing Grant
Postdoctoral Research Fellowship in Microbial Biology for FY 2004
2004财年微生物学博士后研究奖学金
  • 批准号:
    0400819
  • 财政年份:
    2005
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Fellowship Award

相似国自然基金

Research on Quantum Field Theory without a Lagrangian Description
  • 批准号:
    24ZR1403900
  • 批准年份:
    2024
  • 资助金额:
    0.0 万元
  • 项目类别:
    省市级项目
Cell Research
  • 批准号:
    31224802
  • 批准年份:
    2012
  • 资助金额:
    24.0 万元
  • 项目类别:
    专项基金项目
Cell Research
  • 批准号:
    31024804
  • 批准年份:
    2010
  • 资助金额:
    24.0 万元
  • 项目类别:
    专项基金项目
Cell Research (细胞研究)
  • 批准号:
    30824808
  • 批准年份:
    2008
  • 资助金额:
    24.0 万元
  • 项目类别:
    专项基金项目
Research on the Rapid Growth Mechanism of KDP Crystal
  • 批准号:
    10774081
  • 批准年份:
    2007
  • 资助金额:
    45.0 万元
  • 项目类别:
    面上项目

相似海外基金

Doctoral Dissertation Research: Assessing the chewing function of the hyoid bone and the suprahyoid muscles in primates
博士论文研究:评估灵长类动物舌骨和舌骨上肌的咀嚼功能
  • 批准号:
    2337428
  • 财政年份:
    2024
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing the physiological consequences of diet and environment for gorillas in zoological settings
博士论文研究:评估动物环境中大猩猩饮食和环境的生理后果
  • 批准号:
    2341433
  • 财政年份:
    2024
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing weight-gain tendencies in a non-human primates
博士论文研究:评估非人类灵长类动物的体重增加趋势
  • 批准号:
    2341173
  • 财政年份:
    2024
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing the impacts of Pleistocene megaherbivores on vegetation stability and resilience in the Arctic
博士论文研究:评估更新世巨型食草动物对北极植被稳定性和恢复力的影响
  • 批准号:
    2230019
  • 财政年份:
    2023
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing Rural Farmers' Soil Management Practices
博士论文研究:评估农村农民的土壤管理实践
  • 批准号:
    2049491
  • 财政年份:
    2021
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing Variability within Lithic Production Sequences
博士论文研究:评估石块生产序列中的变异性
  • 批准号:
    2133631
  • 财政年份:
    2021
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing Factors that Influence Streamflow
博士论文研究:评估影响水流的因素
  • 批准号:
    2003248
  • 财政年份:
    2020
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing Multiple Lines of Evidence for Gene Flow in Archaeological Contexts
博士论文研究:评估考古背景下基因流动的多重证据
  • 批准号:
    1945986
  • 财政年份:
    2020
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing and Mapping Ecosystem Service Uses and Values Among National Forest Stakeholders
博士论文研究:评估和绘制国家森林利益相关者的生态系统服务用途和价值
  • 批准号:
    1832315
  • 财政年份:
    2018
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
Doctoral Dissertation Research: Assessing the Impact of International Accords on Local Environmental Governance
博士论文研究:评估国际协议对地方环境治理的影响
  • 批准号:
    1832710
  • 财政年份:
    2018
  • 资助金额:
    $ 1.99万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了