Dissecting KDEL receptor clustering at the mammalian cell surface in response tocargo binding, internalization and compartmental transport (A06)

剖析哺乳动物细胞表面 KDEL 受体簇对货物结合、内化和区室运输的反应 (A06)

• 批准号: 228302549
• 金额: --
• 依托单位: Präsidialbüro
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/228302549?language=en
• 关键词: Dissecting; KDEL; receptor; clustering; mammalian

Receptor cluster formation is a ubiquitous phenomenon in pro- and eukaryotic cells to physically sense cargo/ligand interactions and subsequently translate an exogenous signal into a cellular response. As a mechanistic understanding of the molecular principles underlying these processes is still lacking, the major goal of this project is to combine genetic, biochemical and imaging data with theoretical modelling to dissect the underlying molecular processes and to identify cellular components involved in cargo-induced KDEL-receptor cluster formation, internalization and transport from and to the plasma membrane in yeast and mammalian cells.

受体簇形成是原核细胞和真核细胞中普遍存在的现象，以物理感测货物/配体相互作用，并随后将外源信号翻译成细胞应答。由于对这些过程背后的分子原理的机械理解仍然缺乏，该项目的主要目标是将遗传，生物化学和成像数据与理论建模相结合，以剖析潜在的分子过程，并确定参与货物诱导的KDEL-受体簇形成，内化和运输的细胞组分，以及酵母和哺乳动物细胞中的质膜。