Engineered Metal Functionalized TiO2 Nanotube Sensing Platform for Assessment of Pneumonia Volatile Biomarkers

用于评估肺炎挥发性生物标志物的工程金属功能化 TiO2 纳米管传感平台

• 批准号: 1706283
• 负责人: Swomitra Mohanty
• 金额: $ 41.91万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-15 至 2021-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1706283&HistoricalAwards=false
• 关键词: Engineered; Metal; Functionalized; TiO2; Nanotube

A major challenge for global health is the development of portable, low-cost, and non-invasive technologies for the diagnosis of diseases, because the availability of such devices could reduce the number of mortalities caused by infection. For example, pneumonia is a disease that annually takes the lives of approximately one million children under the age of five due to poor access to health care or tools that can rapidly diagnose the disease. The goal of this research project is to develop innovative, non-invasive biosensing methods to detect compounds emitted in the breath of individuals suffering from pneumonia. The researchers will create small tubes of a ceramic material, titanium dioxide, that have diameters on the order of several nanometers and then attach a metal layer to the exterior of the tubes. They will then study how the electrical resistance of the tube changes as a function of its exposure to vapors of compounds that are emitted by microorganisms that cause pneumonia. This research project will leverage known specific molecular interactions between metals and volatile organic biomarkers (VOBs). Such compounds are given off by microorganisms that cause diseases, such as pneumonia, and are found in the breath of infected individuals. Previous research has shown a clear link between VOBs from patient breath and pathological conditions such as diabetes, tuberculous, colorectal cancer, and pneumonia. The research approach uses metals deposited on a titanium dioxide nanotube substrate as a binding element for VOBs of interest. Binding events cause a measurable change in current in the sensor which will be characterized. The project will focus on creating a pneumonia sensor by (1) using computational modeling to understand the interactions between metals or combination of metals with known VOBs associated with pneumonia; (2) creating a metal-modified titanium dioxide nanotube sensing platform capable of detecting VOBs associated with pneumonia; (3) studying the limit of detection of the sensor for the VOBs associated with pneumonia; and (4) developing a sensor for examining the biological relationship between bacterial load in a culture and the amount of VOB generated using Streptococcus pneumonia, Staphylococcus aureus, and Haemophilus influenza (organisms commonly found in pneumonia with known VOBs). These research efforts will demonstrate the utility of the proposed approach and lay the foundation for building a breath-based sensor for assessing pneumonia.

全球卫生面临的一大挑战是开发便携、低成本和非侵入性的疾病诊断技术，因为这种设备的可用可以减少感染造成的死亡人数。例如，肺炎是一种疾病，由于缺乏获得医疗保健或快速诊断疾病的工具，每年约有100万5岁以下儿童丧生。这项研究项目的目标是开发创新的、非侵入性的生物传感方法，以检测肺炎患者呼吸中排放的化合物。研究人员将制造一种名为二氧化钛的陶瓷材料制成的小管子，这种管子的直径约为几纳米，然后在管子的外部附着一层金属。然后，他们将研究管子的电阻如何随着暴露在由引起肺炎的微生物排放的化合物蒸气中而发生变化。这项研究项目将利用已知的金属和挥发性有机生物标记物(VOB)之间的特定分子相互作用。这种化合物是由引起疾病的微生物释放出来的，比如肺炎，并在感染者的呼吸中发现。以前的研究已经表明，患者呼吸中的VOB与糖尿病、结核病、结直肠癌和肺炎等病理条件之间存在明显的联系。该研究方法使用沉积在二氧化钛纳米管衬底上的金属作为感兴趣的VOB的结合元素。结合事件导致传感器中的电流发生可测量的变化，这将被描述。该项目将专注于通过以下方式创建肺炎传感器：(1)使用计算模型来了解金属或金属与已知与肺炎有关的VOB的组合之间的相互作用；(2)创建能够检测与肺炎有关的VOB的金属修饰的二氧化钛纳米管传感平台；(3)研究传感器对与肺炎有关的VOB的检测限度；以及(4)开发传感器，用于检测培养物中的细菌负荷与由肺炎链球菌、金黄色葡萄球菌和流感嗜血杆菌(肺炎中常见的已知VOB产生的微生物)产生的VOB数量之间的生物关系。这些研究工作将证明建议的方法的实用性，并为建立基于呼吸的传感器来评估肺炎奠定基础。

项目成果

Enhancing electrochemical properties of TiO2 nanotubes via engineered defect laden crystal structures

• DOI: 10.1016/j.matlet.2020.127956
• 发表时间: 2020-08-15
• 期刊: MATERIALS LETTERS
• 影响因子: 3
• 作者: Malik, Hammad;Barrera, Kai;Carlson, Krista
• 通讯作者: Carlson, Krista