Illuminating organelle dynamics through highly scattering bone: intravital wavefront optimized structured illumination imaging

通过高度散射的骨骼照亮细胞器动力学：活体波前优化的结构照明成像

• 批准号: 1706916
• 负责人: Luke Mortensen
• 金额: $ 50.17万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1706916&HistoricalAwards=false
• 关键词: Illuminating; organelle; dynamics; through; highly

Mortensen 1706916This proposal describes a range of optical designs and engineering tasks to create an optimized microscope for use in deep tissue imaging. If successful, the proposed work will extend super-resolution structured illumination microscopy substantially and will provide a significant biological advance for tracking and monitoring engineered stem cells in deep tissues. In vivo optical imaging at depth with cellular level resolution is a major challenge to the clinical translation of biophotonic technology and the proposed resolution and depth of penetration metrics would be very beneficial to the field. The PIs support a diverse group of trainees (including high school students) and will develop two new courses on microscopy for biologists and biology for microscopists.The proposed research will benefit a range of fields including the skeletal biology, hematopoietic and neuroscience communities. To enable sub-diffraction microscopy in living bone marrow, three objectives will be pursued: 1.) A dedicated 2-photon microscope will be designed and built with a deformable mirror and digital micromirror device. 2.) The wavefront shaping 2-photon scanning microscope will be enhanced for live 2-photon structured illumination imaging resulting in 150nm lateral resolution. 3.) The instrument will be tested and validated by intravital imaging of mitochondria dynamics in Mesenchymal Stem Cells (MSCs) in mouse bone.

Mortensen 1706916该提案描述了一系列光学设计和工程任务，以创建用于深层组织成像的优化显微镜。如果成功，拟议的工作将大大扩展超分辨率结构照明显微镜，并将为跟踪和监测深层组织中的工程干细胞提供重大的生物学进步。具有细胞水平分辨率的深度处的体内光学成像是生物光子技术的临床转化的主要挑战，并且所提出的分辨率和穿透深度度量将对该领域非常有益。PI支持不同的受训者群体（包括高中生），并将为生物学家开发两门新的显微镜课程，为显微镜学家开发生物学课程。拟议的研究将使包括骨骼生物学，造血和神经科学社区在内的一系列领域受益。为了能够在活骨髓中进行亚衍射显微镜检查，将追求三个目标：1.）一个专用的双光子显微镜将被设计和建造一个可变形的镜子和数字成像设备。2.）的情况。波前整形双光子扫描显微镜将被增强，用于实时双光子结构照明成像，从而产生150nm的横向分辨率。3.）第三章该仪器将通过小鼠骨骼中间充质干细胞（MSC）中线粒体动力学的活体成像进行测试和验证。

项目成果

Second harmonic generation characterization of collagen in whole bone

全骨胶原蛋白的二次谐波产生表征

• DOI: 10.1364/boe.391866
• 发表时间: 2020
• 期刊: Biomedical Optics Express
• 影响因子: 3.4
• 作者: Pendleton, Emily G.;Tehrani, Kayvan F.;Barrow, Ruth P.;Mortensen, Luke J.
• 通讯作者: Mortensen, Luke J.

Intravital multi-photon imaging through intact highly scattering bone using binary wavefront optimization

使用二元波前优化通过完整的高散射骨进行活体多光子成像

• DOI: 10.1364/3d.2018.jtu5b.2
• 发表时间: 2018
• 作者: Tehrani, Kayvan F.;Kner, Peter;Mortensen, Luke J.
• 通讯作者: Mortensen, Luke J.

Characterization of memory effect in juvenile mouse skull for imaging through intact bone

通过完整骨骼成像的幼年小鼠头骨记忆效应的表征

• DOI: 10.1364/boda.2019.jt4a.27
• 发表时间: 2019
• 期刊: Bio-Optics: Design and Application 2019
• 作者: Tehrani, Kayvan Forouhesh;Koukourakis, Nektarios;Czarske, Jürgen;Mortensen, Luke
• 通讯作者: Mortensen, Luke

Characterization of collagen formation surrounding osteocytes using second and third harmonic generation

使用二次和三次谐波生成表征骨细胞周围的胶原形成

• DOI: 10.1117/12.2510814
• 发表时间: 2019
• 期刊: Multimodal Biomedical Imaging XIV
• 作者: Pendleton, Emily G.;Tehrani, Kayvan F.;Barrow, Ruth P.;Mortensen, Luke J.
• 通讯作者: Mortensen, Luke J.

Finite difference time domain modeling of wavefront aberrations in bone using second harmonic generation microscopy

使用二次谐波发生显微镜对骨中波前像差进行有限差分时域建模

• DOI: 10.1117/12.2290376
• 发表时间: 2018
• 期刊: Adaptive Optics and Wavefront Control for Biological Systems IV
• 作者: Tehrani, Kayvan F.;Phang, Sendy;Kner, Peter;Vukovic, Ana;Mortensen, Luke J.
• 通讯作者: Mortensen, Luke J.