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Investigating midbody mRNA function during mitosis

研究有丝分裂期间的中体 mRNA 功能

基本信息

批准号：
1716298
负责人：
Ahna Skop
金额：
$ 75万
依托单位：
University of Wisconsin-Madison
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-01 至 2020-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1716298&HistoricalAwards=false
关键词：
Investigating midbody mRNA function during

项目摘要

Cell division involves two fundamental process. In the first step, genetic information is partitioned to opposite sides of the mother cell, while in the second step, the mother cell physically partitions into two separate daughter cells at a region known as the midbody. This project will investigate the observation that some genetic information accumulates at the midbody. The work employs interdisciplinary approaches that can be widely employed for making rapid progress in understanding the functions of poorly understood cell division genes and mRNAs. The Broader Impact activities include a public outreach component in which microscopy images generated from this research will be used to create a moveable 20-foot scientific art installation called "Genetic Reflections". This piece will be installed in a public, high-traffic area in the UW-Madison Biotech Center and then the Wisconsin Science Museum. Portions of this installation will be constructed to travel locally around the state of Wisconsin and then nationally. The goal is to have scientific artwork that is beautiful, informative and promotes future public engagement projects between scientists and the public.In certain cells types, midbody factor mis-regulation and midbody accumulation leads to altered growth and development, suggesting that the factors (proteins and mRNAs) associated with midbodies when mis-handled by the cell, can lead to dramatic and detrimental proliferative and cell fate changes. Necessary reagents, techniques, and datasets will be generated and shared, providing the foundation to determine how midbody-associated mRNAs are regulated during cell division. With such knowledge, it will be possible to probe the dynamics of midbody-specific mRNAs during mitosis. The PI has made an unexpected preliminary discovery in the midbody transcriptome of an enrichment of the mRNA of an important kinesin widely known to function on the protein level. This discovery challenges current understanding of the mechanisms of molecular motor regulation and transport, and also likely cytokinesis. If successful, the knowledge generated from this research will transform current understanding about the regulation of molecular motors and cell division in all aspects of human and organismal biology.

细胞分裂涉及两个基本过程。在第一步中，遗传信息被分配到母细胞的相对两侧，而在第二步中，母细胞在称为中间体的区域物理地划分为两个独立的子细胞。这个项目将调查一些遗传信息在中体积累的观察结果。这项工作采用了跨学科的方法，可以广泛用于在理解知之甚少的细胞分裂基因和mRNA的功能方面取得快速进展。更广泛的影响活动包括一个公共宣传部分，其中从这项研究中产生的显微镜图像将被用来创建一个可移动的20英尺的科学艺术装置，称为“遗传反射”。这件作品将被安装在一个公共的，高流量的区域在威斯康星大学麦迪逊分校生物技术中心，然后威斯康星州科学博物馆。这个装置的一部分将被建造成在威斯康星州当地旅行，然后在全国范围内旅行。我们的目标是拥有美丽、信息丰富的科学艺术品，并促进科学家和公众之间未来的公众参与项目。在某些细胞类型中，中间体因子的错误调节和中间体积累会导致生长和发育的改变，这表明与中间体相关的因子（蛋白质和mRNA）在被细胞错误处理时会导致戏剧性和有害的增殖和细胞命运变化。必要的试剂，技术和数据集将被生成和共享，为确定细胞分裂过程中中间体相关mRNA的调控方式提供基础。有了这些知识，将有可能探测有丝分裂过程中中间体特异性mRNA的动态。PI在中体转录组中发现了一个意想不到的初步发现，即广泛已知在蛋白质水平上发挥作用的重要驱动蛋白的mRNA富集。这一发现挑战了目前对分子马达调节和运输机制的理解，也可能是胞质分裂。如果成功，这项研究产生的知识将改变目前对人类和生物体生物学各个方面的分子马达和细胞分裂调节的理解。