A biocultural investigation of epigenetics, gene expression and the intergenerational effects of stress in mothers and neonates
表观遗传学、基因表达以及母亲和新生儿压力的代际影响的生物文化研究
基本信息
- 批准号:1719866
- 负责人:
- 金额:$ 49.94万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-06-15 至 2022-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This project will investigate relationships among maternal stress, epigenetic markers, and infant outcomes in a sample of mother-infant pairs in which the mothers experienced extreme stress during pregnancy. The goal of the research is to better understand how epigenetic mechanisms may link stress in one generation and changes in gene expression and later-life outcomes in the next generation. The idea that stress, including psychosocial stress, may be related physiologically to changes in an offspring's outcomes later in life is central to many anthropological models of growth, development, adaptation and plasticity. The research findings also will be potentially informative for understanding the increasing prevalence of stress-related disorders in the United States. This project will support ongoing international research collaborations and integrative graduate training in biological, cultural, and medical anthropology for students from groups underrepresented in STEM fields. One of the most stressful environments today is found in the Democratic Republic of Congo (DRC) where war and post-war conflict has ravaged the country for over 20 years. This project will investigate the role of DNA methylation and gene expression in translating experiences of violence and stress in a sample of mothers into altered well-being outcomes for those mothers and their offspring. The project will therefore advance our understanding of human phenotypic plasticity, a central theme in anthropological, biocultural research. The proposed biocultural approach integrates sophisticated ethnographic, epigenomic, transcriptomic, and hormonal data; a wealth of biological samples, maternal stress exposures and neonate/maternal outcomes have already been collected for 100 mother-infant pairs. The biological samples will be assayed for DNA methylation (Illumina MethylationEPIC BeadChip), gene expression (Agilent SurePrint G3 Human Gene Expression array), and cortisol-based stress measures (ELISA and LC-MS). Associations among maternal stress, DNA methylation, gene expression and neonate/maternal outcomes will be tested using multiple regression analyses, to better understand the biological and molecular mechanisms that underlie adaptive responses to stress. The project will focus on genes involved in the stress process, e.g., HPA axis genes, as well as the entire genome to test for genome-wide effects of stress.
本研究将以母亲在怀孕期间经历极端压力的母婴对为样本,探讨母亲压力、表观遗传标记和婴儿结局之间的关系。这项研究的目的是更好地了解表观遗传机制如何将一代人的压力与下一代基因表达和晚年生活结果的变化联系起来。压力,包括社会心理压力,可能与后代在以后生活中的结果变化有关,这是许多人类学模型的核心,生长,发育,适应和可塑性。研究结果也将为了解美国与压力有关的疾病日益普遍提供潜在的信息。该项目将支持正在进行的国际研究合作,并为来自STEM领域代表性不足的群体的学生提供生物,文化和医学人类学的综合研究生培训。当今最紧张的环境之一是在刚果民主共和国(刚果民主共和国),战争和战后冲突蹂躏了这个国家20多年。该项目将研究DNA甲基化和基因表达在将母亲样本中的暴力和压力经历转化为这些母亲及其后代的幸福结果中的作用。因此,该项目将促进我们对人类表型可塑性的理解,这是人类学和生物文化研究的中心主题。 拟议的生物文化方法整合了复杂的民族志、表观基因组、转录组和激素数据;已经为100对母婴收集了丰富的生物样本、孕产妇压力暴露和新生儿/孕产妇结局。将对生物样本进行DNA甲基化(Illumina MethylationEPIC BeadChip)、基因表达(Agilent SurePrint G3 Human Gene Expression array)和基于皮质醇的应激测量(ELISA和LC-MS)分析。母体压力,DNA甲基化,基因表达和新生儿/母体结果之间的关联将使用多元回归分析进行测试,以更好地了解压力适应性反应的生物和分子机制。该项目将重点关注与压力过程有关的基因,例如,HPA轴基因,以及整个基因组,以测试压力对全基因组的影响。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Associations between Maternal Psychosocial Stress, DNA Methylation, and Newborn Birth Weight Identified by Investigating Methylation at Individual, Regional, and Genome Levels
通过研究个体、区域和基因组水平的甲基化来确定母亲心理社会压力、DNA 甲基化和新生儿出生体重之间的关联
- DOI:
- 发表时间:2019
- 期刊:
- 影响因子:0
- 作者:Clukay, Chris J;Hughes, David A;Kertes, Darlene A;Mulligan, Connie J
- 通讯作者:Mulligan, Connie J
Dehydroepiandrosterone at birth: Response to stress and relation to demographic, pregnancy and delivery factors
- DOI:10.1111/jne.12906
- 发表时间:2020-10-01
- 期刊:
- 影响因子:3.2
- 作者:Kamin, Hayley S.;Bhatt, Samarth S.;Kertes, Darlene A.
- 通讯作者:Kertes, Darlene A.
The emerging field of social and behavioral epigenetic
社会和行为表观遗传学的新兴领域
- DOI:10.1002/9781118900772.etrds0447
- 发表时间:2018
- 期刊:
- 影响因子:0
- 作者:Mulligan, C
- 通讯作者:Mulligan, C
Epigenetics, gene expression, and stress in mothers and offspring in the Democratic Republic of Congo: A biocultural investigation of the intergenerational effects of stress
刚果民主共和国母亲和后代的表观遗传学、基因表达和压力:压力代际影响的生物文化调查
- DOI:
- 发表时间:2019
- 期刊:
- 影响因子:2.8
- 作者:Mulligan CJ, Maisha FM
- 通讯作者:Mulligan CJ, Maisha FM
DNA methylation of methylation complex genes in relation to stress and genome-wide methylation in mother-newborn dyads
- DOI:10.1002/ajpa.23341
- 发表时间:2018-01-01
- 期刊:
- 影响因子:2.8
- 作者:Clukay, Christopher J.;Hughes, David A.;Mulligan, Connie J.
- 通讯作者:Mulligan, Connie J.
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Connie Mulligan其他文献
Donkey Domestication
- DOI:
10.1007/s10437-012-9126-8 - 发表时间:
2013-02-03 - 期刊:
- 影响因子:1.100
- 作者:
Birgitta Kimura;Fiona Marshall;Albano Beja-Pereira;Connie Mulligan - 通讯作者:
Connie Mulligan
Connie Mulligan的其他文献
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{{ truncateString('Connie Mulligan', 18)}}的其他基金
Intergenerational impact of violence exposure during pregnancy on epigenetic change
怀孕期间暴力暴露对表观遗传变化的代际影响
- 批准号:
1849379 - 财政年份:2019
- 资助金额:
$ 49.94万 - 项目类别:
Standard Grant
Doctoral Dissertation Research: Effect of intrauterine environment on newborn telomere length
博士论文研究:宫内环境对新生儿端粒长度的影响
- 批准号:
1540372 - 财政年份:2015
- 资助金额:
$ 49.94万 - 项目类别:
Standard Grant
US/UK Joint Workshop on Social and Behavioral Epigenetics
美国/英国社会和行为表观遗传学联合研讨会
- 批准号:
1448213 - 财政年份:2014
- 资助金额:
$ 49.94万 - 项目类别:
Standard Grant
Doctoral Dissertation Improvement: Testing for archaic hominid introgression in Eritrean and Yemeni modern human genomes
博士论文改进:厄立特里亚和也门现代人类基因组中古代原始人基因渗入的测试
- 批准号:
1258965 - 财政年份:2013
- 资助金额:
$ 49.94万 - 项目类别:
Standard Grant
Epigenetic alterations and stress among new mothers and neonates in the Democratic Republic of Congo: A biocultural investigation of the intergenerational effects of war
刚果民主共和国新妈妈和新生儿的表观遗传改变和压力:战争代际影响的生物文化调查
- 批准号:
1231264 - 财政年份:2012
- 资助金额:
$ 49.94万 - 项目类别:
Standard Grant
Genetic Ancestry, Race, and Health Disparities: A Biocultural Approach
遗传血统、种族和健康差异:生物文化方法
- 批准号:
0820687 - 财政年份:2008
- 资助金额:
$ 49.94万 - 项目类别:
Continuing Grant
Human Dispersals Out of Africa: Mitochondrial and Y chromosomal Genetic Analysis of Eritrean and Omani Populations
人类走出非洲的扩散:厄立特里亚和阿曼人群的线粒体和 Y 染色体遗传分析
- 批准号:
0518530 - 财政年份:2005
- 资助金额:
$ 49.94万 - 项目类别:
Continuing Grant
Acquisition of an automated DNA analysis system
购置自动化 DNA 分析系统
- 批准号:
0129721 - 财政年份:2002
- 资助金额:
$ 49.94万 - 项目类别:
Standard Grant
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