SBIR Phase I: Rapid, instrument-free Nucleic Acid Test for Pathogens and Biothreats

SBIR第一阶段项目将开发一种快速、灵敏的即时（POC）二维纸网络（2DPN）核酸检测原型，用于检测病原体或生物威胁。拟议的产品基于一种称为模板辅助快速测定（塔拉）的颠覆性平台技术，该技术直接对各种生物样品起作用，而无需核酸纯化。塔拉将最少的样品制备与靶标扩增和检测无缝集成，这一直是POC核酸检测中的最大挑战。该测试消除了对用于样本收集、资源密集型冷藏储存和样本运输的多余设备的需求，以及对仪器和实验室的访问（以及RT-qPCR、逆转录聚合酶链反应所需的间接成本），这些测试是目前最常用的病原体或生物威胁检测测试。该公司将通过MERS-CoV检测的例子展示该设备的概念验证。基于微流体的自动化2DPN塔拉卡可广泛应用于感染原或生物威胁的即时或现场核酸检测的应用。2DPN塔拉检测卡提供了易于使用的样本检测系统。

This SBIR Phase I project will develop a prototype for a rapid and sensitive, point-of-care (POC) two-dimensional paper networks (2DPN) nucleic acid test for the detection of pathogens or biothreats. The proposed product is based on a disruptive platform technology called the Template Assisted Rapid Assay (TARA), which works directly on diverse biological samples without nucleic acid purification. TARA seamlessly integrates minimal sample preparation with target amplification and detection, which has been the greatest challenge in POC nucleic acid testing. The test eliminates the need for excess devices for sample collection, resource-intensive refrigerated storage and transport of samples, access to instrumentation and laboratory (with its overhead costs which are required for RT-qPCR, Reverse Transcription Polymerase Chain Reaction) tests that are the most commonly used tests for pathogen or biothreat detection currently. The company will show proof-of-concept of the device via the example of MERS-CoV detection. A microfluidic-based automated 2DPN TARA card can be applied broadly to applications for point-of-care or on-the-field nucleic acid detection of infectious agents or biothreats. The 2DPN TARA card provides the sample to answer, and easy to use, assay system.