MRI/Acquisition: Upgrading the Microbial Ecology and Biotechnology Laboratory at Universidad del Turabo with a more efficient Genetic Analyzer for sequencing and fragment analysis

MRI/采集：使用更高效的基因分析仪进行测序和片段分析，升级图拉博大学的微生物生态学和生物技术实验室

• 批准号: 1726158
• 负责人: Sharon Cantrell
• 金额: $ 21.31万
• 依托单位: Universidad Del Turabo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726158&HistoricalAwards=false
• 关键词: MRI; Acquisition; Upgrading; Microbial; Ecology

An award is made to Universidad del Turabo to upgrade the existing molecular facilities with the acquisition of a more efficient Genetic Analyzer that will enhance current research activities at Universidad del Turabo (UT) and project partners (University of Puerto Rico-Cayey). The project will continue to support the training of undergraduate and graduate students under different research activities and recently funded initiatives to Universidad del Turabo. The project will use Sanger-Sequencing and Fragment Analysis in different projects that will provide baseline data for other research projects. All projects will foster collaboration among project researchers and institutions. The project will also provide training in molecular techniques and in the operation of the state of the art Genetic Analyzer, to augment the future STEM work force through different sponsored projects led by the PI, Co-PI and major instrument users (NSF LTER, NIH-INBRE, USDA and PRSTRT at UT and HHMI SEA-PHAGES of UPR-Cayey). UPR-Cayey has also developed a Research Experiences for Teachers (RET) Program, supported by HHMI that provides training to pre-service teachers (pedagogy majors) in original research in Microbial Ecology that will make use of the Genetic Analyzer. Peer reviewed publications and scientific presentations at local and national meetings will be produced as the results of the different research projects are generated.The requested Genetic Analyzer (ABI3500) provides faster, easier and better quality control for sequencing and fragment analysis. The UT Microbial Ecology and Biotechnology Laboratory (MEBL) has been essential in the training of undergraduate and graduate students, faculty and teachers in the use of genomic techniques such as Sanger Sequencing and Fragment Analysis to study microbial communities. The project brings together seven (7) UT and University of Puerto Rico-Cayey researchers that represent two primary undergraduate minority serving Hispanic institutions. All research projects have demonstrated the need of the new genetic analyzer ABI 3500 and how the instrument will benefit the training of students at all levels, as well as school teachers. The twelve (12) research projects address the following topics: water quality, aeromycology, tropical rain forest microbial ecology, bioremediation, agro-microbiome, bioprospecting, biochemistry (DNA-conjugated quantum dots), Drosophila population genetics, avian DNA barcoding, and genomics of bacteriophages and bacteria. All these themes are very important for the improvement and understanding of climate change, environmental protection and human and environmental health.

授予Universidad del Turabo升级现有的分子设施，收购更高效的遗传分析仪，这将加强Universidad del Turabo（UT）和项目合作伙伴（波多黎各大学-Cayey）的当前研究活动。该项目将继续支持在不同的研究活动和最近资助的倡议下对本科生和研究生的培训。该项目将在不同的项目中使用桑格测序和片段分析，为其他研究项目提供基线数据。所有项目都将促进项目研究人员和机构之间的合作。该项目还将提供分子技术和最先进的遗传分析仪操作方面的培训，以通过PI，Co-PI和主要仪器用户（UT的NSF LTER，NIH-INBRE，USDA和PRSTRT以及UPR-Cayey的HHMI SEA-PHAGES）领导的不同赞助项目来增加未来的STEM工作队伍。UPR Cayey还开发了一个教师研究经验（RET）计划，由HHMI支持，为在职前教师（教育学专业）提供微生物生态学原创研究的培训，将利用遗传分析仪。 随着不同研究项目的结果产生，将在地方和国家会议上发表同行评审的出版物和科学报告。所要求的遗传分析仪（ABI 3500）为测序和片段分析提供更快，更容易和更好的质量控制。UT微生物生态学和生物技术实验室（MEBL）在本科生和研究生，教师和教师使用基因组技术（如桑格测序和片段分析）研究微生物群落的培训中至关重要。该项目汇集了七（7）UT和波多黎各-Cayey大学的研究人员，代表两个主要的本科少数民族服务于西班牙裔机构。 所有研究项目都证明了对新型遗传分析仪ABI 3500的需求，以及该仪器将如何有益于各级学生和学校教师的培训。这12个研究项目涉及以下主题：水质、空气真菌学、热带雨林微生物生态学、生物修复、农业微生物组、生物勘探、生物化学（DNA结合量子点）、果蝇种群遗传学、鸟类DNA条形码以及噬菌体和细菌的基因组学。所有这些主题对于改善和理解气候变化、环境保护以及人类和环境健康都非常重要。