Dimensions: Collaborative Research: Assembly and function of nectar microbial communities

维度：合作研究：花蜜微生物群落的组装和功能

• 批准号: 1737758
• 负责人: Tadashi Fukami
• 金额: $ 145万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1737758&HistoricalAwards=false
• 关键词: Dimensions; Collaborative; Research; Assembly; function

The goal of this project is to study the ecology of micro-organisms (life too small to be easily seen, like yeast and bacteria) that live in the nectar of flowers. Nectar is an interesting place for such organisms to live because it doesn't last long -- most flowers last only a few days. Yet, each flower can be home to a complex and changing "community" of yeast and bacteria; they are miniature ecosystems. Scientists will conduct experiments to explore how those micro-organisms get into nectar and whether the order in which they arrive determines the final set that end up living there. Because each flower contains its own separate ecosystem that can be easily tweaked by scientists, this study system is a good one for understanding how ecosystems of all sizes may develop. Such understanding is important when trying to help preserve rare species and to keep ecosystems functioning in ways that benefit both humans and wildlife. In this project, for example, the particular set of yeast and bacteria that end up in flowers may have a large impact on what pollinators visit flowers and thus on how effectively the flowers are pollinated. Given that a lot of what humans eat depends on pollination of crops, understanding what can lead to more effective pollination has both economic and social relevance. High-school students will be mentored in research through a 7-week summer research internship. Graduate students and postdoctoral fellows will help mentor those high school students and be mentored, themselves. Workshops on bioinformatics will be offered to train students in essential skills of data analysis.A combination of field observations, field experiments, and laboratory experiments will be conducted on nectar microbes associated with a hummingbird-pollinated shrub in California. Data will be integrated through construction of a simulation model of plant-hummingbird-nectar microbe interactions, providing tests of community assembly theory. Researchers will integrate genetic, phylogenetic and functional dimensions of biodiversity by studying how (1) yeast genetic diversity and bacterial phylogenetic diversity are influenced by flowering phenology, (2) yeast genetic diversity and bacterial phylogenetic diversity suppress each other through antagonistic priority effects within floral nectar, (3) yeast genetic diversity and bacterial phylogenetic diversity alter hummingbirds' foraging preference by modifying nectar chemistry as a result of growth in nectar, thereby affecting functional diversity of flowers in terms of nectar chemistry, pollination success, and seed production, and (4) how functional diversity of flowers feeds back to affect yeast genetic diversity and bacterial phylogenetic diversity via altered dispersal of yeast and bacteria across flowers.

这个项目的目标是研究生活在花蜜中的微生物（太小而不容易看到的生命，如酵母和细菌）的生态学。花蜜是这种生物生活的一个有趣的地方，因为它不会持续很长时间-大多数花只持续几天。 然而，每朵花都可能是一个复杂而不断变化的酵母和细菌“群落”的家园;它们是微型生态系统。科学家们将进行实验，探索这些微生物如何进入花蜜，以及它们到达的顺序是否决定了最终生活在那里的最终集合。 因为每朵花都有自己独立的生态系统，科学家可以很容易地调整，所以这个研究系统对于理解各种规模的生态系统如何发展是一个很好的系统。 在试图帮助保护稀有物种和保持生态系统以有利于人类和野生动物的方式运作时，这种理解是重要的。 例如，在这个项目中，最终进入花朵的特定酵母和细菌可能会对授粉者访问花朵的方式产生很大影响，从而影响花朵授粉的有效性。 鉴于人类吃的很多东西都取决于作物的授粉，了解什么可以导致更有效的授粉具有经济和社会意义。 高中生将通过为期7周的暑期研究实习进行研究指导。研究生和博士后研究员将帮助指导这些高中生，并得到指导，自己。 通过野外观察、野外实验和室内实验相结合的方法，对加州一种蜂鸟授粉灌木花蜜中的微生物进行研究。数据将通过构建植物-蜂鸟-花蜜微生物相互作用的模拟模型进行整合，为群落组装理论提供测试。研究人员将通过研究（1）开花物候如何影响酵母遗传多样性和细菌系统发育多样性，（2）酵母遗传多样性和细菌系统发育多样性通过花蜜中的拮抗优先效应相互抑制，（3）酵母遗传多样性和细菌系统发育多样性通过改变花蜜中的化学物质而改变蜂鸟的觅食偏好，从而在花蜜化学、授粉成功和种子生产方面影响花的功能多样性，以及（4）花的功能多样性如何反馈以通过改变酵母和细菌在花中的分散来影响酵母遗传多样性和细菌系统发育多样性。

项目成果

Contrasting effects of yeasts and bacteria on floral nectar traits

• DOI: 10.1093/aob/mcy032
• 发表时间: 2018-06
• 期刊: Annals of Botany
• 影响因子: 4.2
• 作者: R. Vannette;T. Fukami

Genomic diversity of a nectar yeast clusters into metabolically, but not geographically, distinct lineages

• DOI: 10.1111/mec.14535
• 发表时间: 2018-04-01
• 期刊: MOLECULAR ECOLOGY
• 影响因子: 4.9
• 作者: Dhami, Manpreet K.;Hartwig, Thomas;Fukami, Tadashi
• 通讯作者: Fukami, Tadashi

Applying modern coexistence theory to priority effects

• DOI: 10.1073/pnas.1803122116
• 发表时间: 2019-03-26
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Grainger, Tess Nahanni;Letten, Andrew D.;Fukami, Tadashi
• 通讯作者: Fukami, Tadashi

Eco-evolutionary buffering: rapid evolution facilitates regional species coexistence despite local priority effects

• DOI: 10.1086/697187
• 发表时间: 2016-02
• 期刊: bioRxiv
• 作者: Meike J. Wittmann;T. Fukami

Nitrogen Assimilation Varies Among Clades of Nectar- and Insect-Associated Acinetobacters

• DOI: 10.1007/s00248-020-01671-x
• 发表时间: 2021-01-06
• 期刊: MICROBIAL ECOLOGY
• 影响因子: 3.6
• 作者: Alvarez-Perez, Sergio;Tsuji, Kaoru;Lievens, Bart
• 通讯作者: Lievens, Bart