Role of receptor specificity and membrane fusion activity of influenza viruses in host range, cell tropism and pathogenicity (B02)

该项目将使用一组定义明确的重组甲型流感病毒（IV）来研究病毒血凝素（HA）和神经氨酸酶（NA）的特性如何影响病毒感染、细胞毒性和诱导人类先天免疫系统细胞（单核细胞、树突状细胞、巨噬细胞和中性粒细胞）的生物反应。作为第二个目标，该项目将表征1979年在欧洲发生的禽H1N1病毒对猪的自然传播和适应过程中HA和NA中16个氨基酸取代的表型，并将确定这些取代中哪些对于新的猪适应病毒的出现至关重要。

The project will use a panel of well-defined recombinant influenza A viruses (IV) to study how properties of viral hemagglutinin (HA) and neuraminidase (NA) affect viral infection, cytotoxicity and induction of biological responses in cells of human innate immune system (monocytes, dendritic cells, macrophages and neutrophils). As a second goal, the project will characterize phenotypes of 16 amino acid substitutions in the HA and NA that occurred during natural transmission and adaptation of an avian H1N1 virus to swine in Europe in 1979 and will identify which of these substitutions were critical for the emergence of the novel swine-adapted virus.