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Workshop: Best Practices for using Next Generation Sequencing (NGS) Datasets to determine Robust Evidence of Positive Selection and Convergent Evolution of Polar Organisms.

研讨会：使用下一代测序 (NGS) 数据集确定极地生物正向选择和趋同进化的有力证据的最佳实践。

基本信息

批准号：
1744877
负责人：
Scott Santagata
金额：
$ 4.93万
依托单位：
Long Island University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-15 至 2021-11-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1744877&HistoricalAwards=false
关键词：
Workshop Best Practices using Next

项目摘要

This workshop aims to bring together a range of investigators to advance and coordinate cross-disciplinary approaches for determining genetic adaptations in polar organisms using datasets derived from Next Generation Sequencing of organisms DNA (genomes) and RNA (trasncriptomes). In particular, this workshop will focus on marine invertebrates as a broad taxonomic grouping and focus on the following efforts. 1) Establishing collaborative research activities that can test for genes under positive selection from diverse polar organisms using genomic and transcriptomic datasets. 2) evaluating current analytical methods for determining positively selected genes among diverse marine organisms 3) exploring current and novel methodologies for detecting genetic modifications acquired through convergent evolution in response to similar environmental conditions and 4) reviewing lab-based protocols for demonstrating the potential functions of candidate genes identified as being positively selected for by Antarctic environments. The workshops will also focus discussions and recommendations to the broader scientific community regarding techniques amenable to freezing or otherwise preserving samples for future sequencing inquires. The workshop coordinators will be advertised broadly and recruit underrepresented groups to participate. Following workshop activities a final report will be published on the outcomes of the efforts.

该研讨会旨在汇集一系列研究人员，以推进和协调跨学科方法，使用来自生物DNA（基因组）和RNA（转录组）的下一代测序的数据集来确定极地生物的遗传适应。特别是，该讲习班将侧重于海洋无脊椎动物作为一个广泛的分类学分组，并侧重于以下努力。 1)建立合作研究活动，可以使用基因组和转录组数据集测试来自不同极地生物的正选择基因。2)评价目前用于确定不同海洋生物中正选择基因的分析方法; 3）探索目前和新的方法，以检测在类似环境条件下通过趋同进化获得的遗传修饰; 4）审查以实验室为基础的方案，以证明被南极环境确定为正选择的候选基因的潜在功能。这些讲习班还将重点讨论和向更广泛的科学界提出建议，涉及适合于冷冻或以其他方式保存样本以供未来测序查询的技术。将广泛宣传讲习班协调员，并招募代表性不足的群体参加。讲习班活动结束后，将发表一份关于工作成果的最后报告。