Workshop: Best Practices for using Next Generation Sequencing (NGS) Datasets to determine Robust Evidence of Positive Selection and Convergent Evolution of Polar Organisms.

这次研讨会的目的是聚集一系列研究人员，利用来自下一代生物DNA(基因组)和RNA(转录)测序的数据集，推进和协调确定极地生物遗传适应的跨学科方法。特别是，这次研讨会将把海洋无脊椎动物作为一个广泛的分类类别加以重点介绍，并着重于以下努力。1)建立合作研究活动，利用基因组和转录组数据集从不同的极地生物中检测正选择下的基因。2)评价在不同海洋生物中确定正向选择基因的现有分析方法；3)探索当前和新的方法，以检测在类似环境条件下通过聚合进化获得的基因修改；以及4)审查以实验室为基础的协议，以证明被南极环境确定为正向选择的候选基因的潜在功能。研讨会还将集中讨论并向更广泛的科学界提出建议，讨论可冻结或以其他方式保存样本以供将来进行测序查询的技术。讲习班协调员将被广泛宣传，并招募代表人数不足的团体参加。在讲习班活动之后，将发表一份关于努力成果的最后报告。

This workshop aims to bring together a range of investigators to advance and coordinate cross-disciplinary approaches for determining genetic adaptations in polar organisms using datasets derived from Next Generation Sequencing of organisms DNA (genomes) and RNA (trasncriptomes). In particular, this workshop will focus on marine invertebrates as a broad taxonomic grouping and focus on the following efforts. 1) Establishing collaborative research activities that can test for genes under positive selection from diverse polar organisms using genomic and transcriptomic datasets. 2) evaluating current analytical methods for determining positively selected genes among diverse marine organisms 3) exploring current and novel methodologies for detecting genetic modifications acquired through convergent evolution in response to similar environmental conditions and 4) reviewing lab-based protocols for demonstrating the potential functions of candidate genes identified as being positively selected for by Antarctic environments. The workshops will also focus discussions and recommendations to the broader scientific community regarding techniques amenable to freezing or otherwise preserving samples for future sequencing inquires. The workshop coordinators will be advertised broadly and recruit underrepresented groups to participate. Following workshop activities a final report will be published on the outcomes of the efforts.