Workshop: Best Practices for using Next Generation Sequencing (NGS) Datasets to determine Robust Evidence of Positive Selection and Convergent Evolution of Polar Organisms.

本次研讨会旨在汇集一系列研究人员，利用下一代生物DNA（基因组）和RNA（转录组）测序数据集，推进和协调确定极地生物遗传适应性的跨学科方法。特别地，这次研讨会将把重点放在海洋无脊椎动物作为一个广泛的分类分组上，并将重点放在以下工作上。1)建立合作研究活动，利用基因组和转录组学数据集检测不同极地生物正选择下的基因。2)评估目前在不同海洋生物中确定正选择基因的分析方法；3)探索当前的和新的方法来检测通过响应类似环境条件的趋同进化获得的遗传修饰；4)审查基于实验室的方案，以证明被确定为南极环境正选择的候选基因的潜在功能。讲习班还将集中讨论并向更广泛的科学界提出关于适用于冷冻或以其他方式保存样品的技术的建议，以供未来测序调查。将广泛宣传讲习班协调员，并招募代表性不足的群体参加。讲习班活动结束后，将发表一份关于努力成果的最后报告。

This workshop aims to bring together a range of investigators to advance and coordinate cross-disciplinary approaches for determining genetic adaptations in polar organisms using datasets derived from Next Generation Sequencing of organisms DNA (genomes) and RNA (trasncriptomes). In particular, this workshop will focus on marine invertebrates as a broad taxonomic grouping and focus on the following efforts. 1) Establishing collaborative research activities that can test for genes under positive selection from diverse polar organisms using genomic and transcriptomic datasets. 2) evaluating current analytical methods for determining positively selected genes among diverse marine organisms 3) exploring current and novel methodologies for detecting genetic modifications acquired through convergent evolution in response to similar environmental conditions and 4) reviewing lab-based protocols for demonstrating the potential functions of candidate genes identified as being positively selected for by Antarctic environments. The workshops will also focus discussions and recommendations to the broader scientific community regarding techniques amenable to freezing or otherwise preserving samples for future sequencing inquires. The workshop coordinators will be advertised broadly and recruit underrepresented groups to participate. Following workshop activities a final report will be published on the outcomes of the efforts.