Physiological Ecology of "Herbivorous" Antarctic Copepods

“草食性”南极桡足类的生理生态学

• 批准号: 1746087
• 负责人: Ann Tarrant
• 金额: $ 65.86万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1746087&HistoricalAwards=false
• 关键词: Physiological; Ecology; Herbivorous; Antarctic; Copepods

Animals in the polar oceans have adapted to dramatic seasonal changes in day length, food availability, and ice cover, as well as to consistently cold waters. This project focuses on the adaptations of copepods - small animals that live in the water column and are an important food source to many different predators. The field studies will take place in the western Antarctic Peninsula, an environment and ecosystem that is rapidly changing. Antarctic copepods have developed particular feeding and behavioral strategies to survive in their very seasonal environment, however it is not known how each of these species will respond to environmental change. The overall goal of this project is to examine and compare these adaptations across species and to understand how each species responds to short-term changes in food availability. The project contains three main objectives: the first objective is to compare the sets of genes across species, especially looking at genes related to storage of energy from food. The second objective is to measure and compare the responses of copepods to changes in food availability. The third objective is to determine how variation across the western Antarctic Pensinsula habitat affects the feeding condition of the copepods. To make the data more useful to the broader research community, a database will be developed enabling easy comparison of genetic information between copepod species. This project will provide hands-on training opportunities to graduate and undergraduate student and will seek to recruit students from underrepresented groups. Results and scientific concepts will be shared through outreach activities, including an expedition blog, a series of interactive animations, and public presentations.Polar marine organisms have adapted to dramatic seasonal changes in photoperiod, light intensity, and ice cover, as well as to cold but stable thermal environments. The western Antarctic Peninsula, the focal region for the field studies, has experienced rapid warming and ice melt. While it is difficult to predict exactly how physical conditions in this region will change, effects on species distributions have already been documented. Large Antarctic copepods in the families Calanidae and Rhincalanidae are dominant components of the mesozooplankton that use different metabolic and behavioral strategies to optimize their use of a highly seasonal food supply. The overall goal of this project is to leverage molecular approaches to examine the physiological and metabolic adaptations at the individual and species level. The project focuses on three main objectives: the first objective is to characterize the gene complement and stage-specific gene expression patterns in Antarctic copepods within an evolutionary context. The second objective is to measure and compare the physiological and molecular responses of juvenile copepods to variable feeding conditions. The third objective is to characterize metabolic variation within natural copepod populations. The metabolically diverse Antarctic copepods also provide an excellent opportunity to compare mechanisms regulating energy storage and utilization and to test hypotheses regarding the roles of specific genes. The field studies will aim to utilize information from an ongoing long term research program (the Palmer Long-Term Ecological Research), which complements the ongoing program and provides extensive context for this project. To make the data more useful to the research community, a database will be developed facilitating comparison of transcriptomes between copepod species. This project will provide hands-on training opportunities to graduate and undergraduate students. Efforts will be made to recruit students who are members of underrepresented minorities. Results and scientific concepts will be broadly disseminated through an expedition blog, a series of interactive animations, and public presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

极地海洋中的动物已经适应了白天长度、食物供应和冰盖的季节性变化，以及持续寒冷的沃茨。该项目的重点是桡足类的适应性-生活在水柱中的小动物，是许多不同掠食者的重要食物来源。实地研究将在南极洲西部半岛进行，那里的环境和生态系统正在迅速变化。南极桡足类已经发展出特殊的摄食和行为策略，以在季节性环境中生存，但尚不清楚这些物种将如何应对环境变化。该项目的总体目标是检查和比较不同物种的这些适应性，并了解每个物种如何应对食物供应的短期变化。该项目包含三个主要目标：第一个目标是比较不同物种的基因组，特别是与食物能量储存有关的基因。第二个目标是测量和比较桡足类对食物供应变化的反应。第三个目标是确定整个南极西部Penslavia栖息地的变化如何影响桡足类的摄食条件。 为了使这些数据对更广泛的研究界更有用，将建立一个数据库，以便容易比较桡足类物种之间的遗传信息。该项目将为研究生和本科生提供实践培训机会，并将寻求从代表性不足的群体中招收学生。将通过外联活动分享成果和科学概念，包括一个探险博客、一系列互动动画和公开演示。极地海洋生物已经适应了光周期、光照强度和冰盖的剧烈季节变化，以及寒冷但稳定的热环境。南极西部半岛是实地研究的重点区域，经历了快速变暖和冰融化。虽然很难准确预测该地区的物理条件将如何变化，但对物种分布的影响已经有了记录。在家庭哲水蚤和Rhincalanidae的大型南极桡足类是主要组成部分的中型浮游动物，使用不同的代谢和行为策略，以优化其使用的高度季节性的食物供应。该项目的总体目标是利用分子方法来研究个体和物种水平的生理和代谢适应。该项目侧重于三个主要目标：第一个目标是在进化背景下描述南极桡足类的基因互补和阶段特异性基因表达模式。第二个目标是测量和比较的生理和分子反应的少年桡足类可变的喂养条件。第三个目标是表征自然桡足类种群内的代谢变异。代谢多样的南极桡足类也提供了一个很好的机会，比较调节能量储存和利用的机制，并测试有关特定基因的作用的假设。实地研究的目的是利用正在进行的长期研究计划（帕尔默长期生态研究）的信息，该计划补充了正在进行的计划，并为该项目提供了广泛的背景。为了使数据对研究界更有用，将开发一个数据库，以便于比较桡足类物种之间的转录组。该项目将为研究生和本科生提供实践培训机会。将努力招收代表性不足的少数民族学生。结果和科学概念将通过一个探险博客，一系列互动动画和公共演示广泛传播。该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

A Crude Awakening: Effects of Crude Oil on Lipid Metabolism in Calanoid Copepods Terminating Diapause

原油觉醒：原油对终止滞育的桡足类脂质代谢的影响

• DOI: 10.1086/705234
• 发表时间: 2019
• 期刊: The biological bulletin
• 作者: Skottene, E;Tarrant, AM;Olsen, AJ;Altin, D;Hansen, BH;Choquet, M;Olsen, RE;Jenssen, BM
• 通讯作者: Jenssen, BM

De novo transcriptome assembly of the Southern Ocean copepod Rhincalanus gigas sheds light on developmental changes in gene expression

• DOI: 10.1016/j.margen.2021.100835
• 发表时间: 2021-07-01
• 期刊: MARINE GENOMICS
• 影响因子: 1.9
• 作者: Berger, Cory A.;Steinberg, Deborah K.;Tarrant, Ann M.
• 通讯作者: Tarrant, Ann M.

The beta-oxidation pathway is downregulated during diapause termination in Calanus copepods

桡足类滞育终止期间β-氧化途径下调

• DOI: 10.1038/s41598-019-53032-5
• 发表时间: 2019
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Skottene, E;Tarrant, AM;Olsen, AJ;Altin, D;Østensen, M-A;Hansen, BH;Choquet, M;Jenssen, BM;Olsen, RE
• 通讯作者: Olsen, RE