Spatially resolved analysis of photobioelectrochemical reactions on quantum dot based electrode architectures

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The aim of this joint research project is the fabrication of new photobioelectrochemical sensing architectures and their detailed characterization. For this purpose the coupling of quantum dots (QDs) with electrodes and specific enzyme reactions at the QDs shall be utilized. It is planned to investigate all subcomponents regarding their individual characteristics in a multiple sensor application. This starts with the colloidal nanoparticles (NPs). Beside QDs also hybrid NPs will be utilized in order to control specifically the detection sensitivity for certain substances. Further, the QD electrode interface will be inspected in more detail and various electrode designs including hydrid multilayers of inorganic semiconductors NPs will be examined. A major topic is the defined immobilization of various enzymes with high activity and controlled spatial resolution on a QD electrode. The properties of such biohybrid systems shall be examined in terms of their potential use for analytical sensor applications. One aspect is the embedding of additional metallic particles in the enzyme layer in order to locally control the temperature by irradiation (plasmonic heating). This may lead to improved electrode signals at the QD electrodes. In addition to systems that are based on the consumption of oxygen or the generation of hydrogen peroxide by the immobilized enzymes, also the coupling of generated charge carriers within the QDs and the redox reactions in the enzymes will be studied. To this end, an improved interface design will be developed based on polymers. Finally, it is planned to build a multiple sensor system, in which different enzymes are spatially separated bound onto a working electrode coated with QDs. The substrate conversion at the enzymes shall be read out selectively by means of a light pointer, hence enabling a parallel analysis of various analytes.