Spatially resolved analysis of photobioelectrochemical reactions on quantum dot based electrode architectures

基于量子点的电极结构上光生物电化学反应的空间分辨分析

• 批准号: 231443326
• 负责人: Professor Dr. Fred Lisdat
• 金额: --
• 依托单位: Fachbereich Ingenieur- und Naturwissenschaften (INW)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/231443326?language=en
• 关键词: Spatially; resolved; analysis; photobioelectrochemical; reactions

The aim of this joint research project is the fabrication of new photobioelectrochemical sensing architectures and their detailed characterization. For this purpose the coupling of quantum dots (QDs) with electrodes and specific enzyme reactions at the QDs shall be utilized. It is planned to investigate all subcomponents regarding their individual characteristics in a multiple sensor application. This starts with the colloidal nanoparticles (NPs). Beside QDs also hybrid NPs will be utilized in order to control specifically the detection sensitivity for certain substances. Further, the QD electrode interface will be inspected in more detail and various electrode designs including hydrid multilayers of inorganic semiconductors NPs will be examined. A major topic is the defined immobilization of various enzymes with high activity and controlled spatial resolution on a QD electrode. The properties of such biohybrid systems shall be examined in terms of their potential use for analytical sensor applications. One aspect is the embedding of additional metallic particles in the enzyme layer in order to locally control the temperature by irradiation (plasmonic heating). This may lead to improved electrode signals at the QD electrodes. In addition to systems that are based on the consumption of oxygen or the generation of hydrogen peroxide by the immobilized enzymes, also the coupling of generated charge carriers within the QDs and the redox reactions in the enzymes will be studied. To this end, an improved interface design will be developed based on polymers. Finally, it is planned to build a multiple sensor system, in which different enzymes are spatially separated bound onto a working electrode coated with QDs. The substrate conversion at the enzymes shall be read out selectively by means of a light pointer, hence enabling a parallel analysis of various analytes.

该联合研究项目的目的是制造新的光电生物电化学传感结构并对其进行详细的表征。为此，应利用量子点与电极的耦合和量子点上的特定酶反应。计划在多传感器应用中调查所有子组件各自的特性。这从胶体纳米颗粒(NPs)开始。除了量子点之外，还将使用混合纳米粒子来具体控制对某些物质的检测灵敏度。此外，将更详细地检查量子点电极界面，并将检查各种电极设计，包括无机半导体纳米颗粒的氢化多层膜。一个主要的主题是在QD电极上固定各种具有高活性和可控空间分辨率的酶。应根据分析传感器应用的潜在用途来审查这类生物杂交系统的特性。一个方面是在酶层中嵌入额外的金属颗粒，以便通过辐射(等离子体加热)局部控制温度。这可能导致在QD电极处改善电极信号。除了基于固定化酶消耗氧气或产生过氧化氢的系统外，还将研究量子点内生成的电荷载流子与酶中的氧化还原反应的耦合。为此，将基于聚合物开发一种改进的界面设计。最后，计划建立一个多传感器系统，在该系统中，不同的酶被空间分离绑定到覆盖有量子点的工作电极上。酶的底物转化应通过光指示器选择性地读出，从而能够对各种分析物进行并行分析。

项目成果

Light as Trigger for Biocatalysis: Photonic Wiring of Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase to Quantum Dot-Sensitized Inverse Opal TiO2 Architectures via Redox Polymers

• DOI: 10.1021/acscatal.8b00951
• 发表时间: 2018-06-01
• 期刊: ACS CATALYSIS
• 影响因子: 12.9
• 作者: Riedel, Marc;Parak, Wolfgang J.;Lisdat, Fred
• 通讯作者: Lisdat, Fred

Bombardment induced ion transport - part IV: ionic conductivity of ultra-thin polyelectrolyte multilayer films.

• DOI: 10.1039/c5cp04004c
• 发表时间: 2016-02
• 期刊: Physical chemistry chemical physics : PCCP
• 作者: Veronika Wesp;M. Hermann;M. Schäfer;Jonas Hühn;W. Parak;K. Weitzel

Dynamic Extracellular Imaging of Biochemical Cell Activity Using InGaN/GaN Nanowire Arrays as Nanophotonic Probes

• DOI: 10.1002/adfm.201802503
• 发表时间: 2018-09-26
• 期刊: ADVANCED FUNCTIONAL MATERIALS
• 影响因子: 19
• 作者: Hoelzel, Sara;Zyuzin, Mikhail V.;Eickhoff, Martin
• 通讯作者: Eickhoff, Martin

Structure and Thermal Stability of Stilbenedithiol SAMs on Au(111)

Au(111) 上二苯乙烯二硫醇 SAM 的结构和热稳定性

• DOI: 10.1002/pssa.201700859
• 期刊: physica status solidi (a)
• 作者: J. Völkner;A. Bashir;W. J. Parak;G. Witte
• 通讯作者: G. Witte

Ion transport through polyelectrolyte multilayers.

• DOI: 10.1002/marc.201300571
• 发表时间: 2013-12
• 期刊: Macromolecular rapid communications
• 影响因子: 4.6
• 作者: S. Carregal-Romero;P. Rinklin;S. Schulze;M. Schäfer;A. Ott;Dominik Hühn;Xian Yu;B. Wolfrum;K. Weitzel;W. Parak