Quantifying the roles of pollination and post-pollination barriers in flowering plant speciation: a case study of the diverse Neotropical genus Burmeistera (Campanulaceae)

量化授粉和授粉后障碍在开花植物物种形成中的作用：以多种新热带植物属（桔梗科）为例

• 批准号: 1754802
• 负责人: Nathan Muchhala
• 金额: $ 73.14万
• 依托单位: University of Missouri-Saint Louis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-06-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1754802&HistoricalAwards=false
• 关键词: Quantifying; roles; pollination; post; barriers

This project will investigate how flowering plants develop reproductive boundaries among species over time. Animal pollinators are critical to the reproduction and survival of most flowering plants, including many of our agricultural crops. Conventional wisdom holds that animal pollinators also have a primary role in driving the diversification of these species. However, this idea has rarely been tested in detail and at scale. Researchers will test this hypothesis using Burmeistera, a flowering plant genus whose 116 species depend on nectar-feeding bats for pollination. Burmeistera species place their pollen on different parts of the bats' heads, which may reduce the exchange of pollen and genes between species. They also have different degrees of genetic incompatibility with each other. These genetic barriers may reduce the fertilization success of cross-species pollinations or the survival of hybrid offspring. Researchers will evaluate which of these reproductive barriers contributes to each stage of the speciation process in Burmeistera. Undergraduate and graduate students, including those from underrepresented groups, will be involved in all aspects of the project and will be trained in field and laboratory techniques. The project will also support the professional development of a post-doctoral researcher. Researchers will communicate results to the public via educational displays at the Missouri Botanical Garden and bilingual field guides for Burmeistera species.This project will discover how reproductive barriers evolve during angiosperm speciation using an integrated experimental and comparative approach. Researchers will construct a robust species-level phylogeny for Burmeistera (Campanulaceae, the bell-flower family) using DNA sequence data from whole plastomes and over 700 nuclear loci. They will then measure pre- and post-pollination reproductive barriers among pairs of species having different phylogenetic distances. Assessment will include cross-pollination trials by captive nectar-feeding bats as well as hand-crosses of species in cultivation. Researchers will compare these measures within the context of the phylogeny to test the hypothesis that post-pollination reproductive barriers evolve before others. They will also determine whether changes in the length of pollen-bearing and receiving organs of Burmeistera flowers are correlated with speciation events, which would be consistent with the hypothesis of pollinator-driven speciation. Project outcomes will include an exceptionally detailed exploration of angiosperm speciation processes, improved taxonomic circumscriptions within a poorly-studied Neotropical flowering plant group, and the creation of new genetic resources and herbarium collections. The project will strengthen international research collaborations among US, Ecuadorian and Colombian scientists.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个项目将研究开花植物如何随着时间的推移发展物种之间的生殖界限。动物传粉者对大多数开花植物的繁殖和生存至关重要，包括我们的许多农作物。传统观点认为，动物传粉者在推动这些物种多样化方面也发挥着主要作用。然而，这一想法很少得到详细和大规模的测试。研究人员将使用Burmeistera来验证这一假设，Burmeistera是一种开花植物属，其116种依赖于花蜜喂养的蝙蝠授粉。Burmeistera物种将它们的花粉放在蝙蝠头部的不同部位，这可能会减少物种之间的花粉和基因交换。它们之间也有不同程度的遗传不相容性。这些遗传障碍可能会降低跨物种授粉的受精成功率或杂交后代的存活率。研究人员将评估哪些生殖障碍有助于Burmeistera物种形成过程的每个阶段。本科生和研究生，包括来自代表性不足群体的学生，将参与项目的各个方面，并将接受实地和实验室技术培训。该项目还将支持博士后研究人员的专业发展。研究人员将通过密苏里州植物园的教育展示和Burmeistera物种的双语实地指南向公众传达结果。该项目将使用综合实验和比较方法来发现被子植物物种形成过程中生殖障碍是如何演变的。研究人员将利用来自整个质体和700多个核基因座的DNA序列数据，为Burmeistera（桔梗科，钟形花家族）构建一个强大的物种水平遗传学。然后，他们将测量具有不同系统发育距离的物种对之间的授粉前和授粉后生殖障碍。评估将包括由圈养的花蜜喂养蝙蝠进行的异花授粉试验以及种植中物种的人工杂交。研究人员将比较这些措施的背景下，授粉后的生殖障碍进化之前，其他测试的假设。他们还将确定Burmeistera花的花粉承载和接收器官长度的变化是否与物种形成事件相关，这与传粉者驱动的物种形成的假设一致。项目成果将包括对被子植物物种形成过程进行非常详细的探索，改进研究不足的新热带区开花植物群的分类环境，以及创建新的遗传资源和植物标本集。该项目将加强美国、厄瓜多尔和哥伦比亚科学家之间的国际研究合作。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Burmeistera quimiensis (Lobelioideae, Campanulaceae): A new species from the Cordillera del Cóndor range in southeast Ecuador

Burmeistera quimiensis（半边莲科、桔梗科）：来自厄瓜多尔东南部科迪勒拉德尔康多尔山脉的新种

• DOI: 10.11646/phytotaxa.433.1.7
• 发表时间: 2020
• 期刊: Phytotaxa
• 影响因子: 1.1
• 作者: MASHBURN, BROCK;PÉREZ, ÁLVARO J.;PERSSON, CLAES;ZAPATA, NICOLÁS;CEVALLOS, DANIELA;MUCHHALA, NATHAN
• 通讯作者: MUCHHALA, NATHAN

Six New Species of Burmeistera (Campanulaceae) from Ecuador

厄瓜多尔六种桔梗科新种

• DOI: 10.3417/2021589
• 发表时间: 2021
• 期刊: A Journal for Botanical Nomenclature
• 作者: Mashburn, Brock;Ulloa Ulloa, Carmen;Muchhala, Nathan
• 通讯作者: Muchhala, Nathan

Utility of targeted sequence capture for phylogenomics in rapid, recent angiosperm radiations: Neotropical Burmeistera bellflowers as a case study

• DOI: 10.1016/j.ympev.2020.106769
• 发表时间: 2020-11-01
• 期刊: MOLECULAR PHYLOGENETICS AND EVOLUTION
• 影响因子: 4.1
• 作者: Bagley, Justin C.;Uribe-Convers, Simon;Muchhala, Nathan
• 通讯作者: Muchhala, Nathan

Three New Species of Burmeistera (Campanulaceae) Endemic to Ecuador

厄瓜多尔特有的缅甸树（桔梗科）三个新种

• DOI: 10.11646/phytotaxa.490.3.3
• 发表时间: 2021
• 期刊: Phytotaxa
• 影响因子: 1.1
• 作者: MUCHHALA, NATHAN;MASHBURN, BROCK
• 通讯作者: MASHBURN, BROCK