Collaborative Research: Spatial analysis of genetic differences in salinity tolerance resulting from rapid natural selection in estuarine oysters
合作研究:河口牡蛎快速自然选择导致的耐盐性遗传差异的空间分析
基本信息
- 批准号:1756698
- 负责人:
- 金额:$ 29.17万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-12-15 至 2022-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Many marine animals have a bipartite life cycle consisting of a stationary bottom-dwelling adult stage and a mobile larval stage. The flow of water transports these larval offspring, and their genes, to different habitat patches. It is thought that animals from nearby patches will be more genetically similar than animals in patches that are further in proximity, but these patterns of genetic similarity may not be maintained if the nearby patches have different habitat characteristics. This idea is fundamental to our understanding of adaptation and evolution, but it has not been adequately tested with respect to the effects of rapid selection. This study applies new technologies to test if the genetic signatures of marine animals change even when patches with different environmental characteristics are closer together than the dispersal distance of larvae. This research focuses on eastern oysters (Crassostrea virginica) in Delaware Bay, and their ability to withstand variability in the amount of salt in the water. This study will provide new insights on factors that control oyster survival and growth in estuaries with different salinity profiles. The three investigators are sharing study results with resource managers and stakeholders to improve shellfish restoration and oyster stock management in Delaware Bay, Chesapeake Bay, and New York. A postdoctoral scholar at Cornell and graduate student at the University of Maryland are being trained and mentored during the project. The investigators are also working with teacher training programs in New York and New Jersey to develop and disseminate new curriculum materials on oyster ecology for middle-school students.The project will investigate whether hyposalinity tolerance of oysters is a function of viability selection during larval dispersal and after settlement. Gene flow across salinity zones within an estuary is expected to be high enough that adaptive differentiation will not result from Darwinian multigenerational processes. Instead, recurrent viability selection in each generation is expected to generate spatial variation in this trait at small spatial scales. This type of recurrent within-generation adaptation has been referred to as phenotype-environment mismatches and has been hypothesized to generate balanced polymorphisms, but it has never been studied beyond single gene cases. The project team is testing for spatially discrete patterns of selection by first collecting oysters from different salinity zones, measuring variation in their tolerance to low salinity and then testing for associations between this trait and genomic variation using whole genome sequencing. Experimental hyposalinity challenges enable within-generation, before/after genomic comparisons to identify DNA variants that change as a result of strong viability selection. Candidate genes and selectively neutral control loci will be assayed in larval, juvenile, and adult samples from the same salinity zones to test for an association between variation at candidate loci and lifetime hyposalinity exposure. Two years of environmental data will be collected and added to an existing long-term data set to map salinity variation. The observed spatial distribution of hyposalinity tolerance and genomic variation associated with it provide a test that could definitively reject the prevalent assumption that all larvae have similar capabilities. If larvae differ by parental source for traits that differentially affect their viability in the plankton, then phenotype-environment mismatches can have profound consequences for population connectivity. This project improves understanding about mechanisms that shape realized larval dispersal and recruitment variation in oyster populations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
许多海洋动物的生命周期分为两部分:固定在海底生活的成虫阶段和活动的幼虫阶段。水流将这些幼虫的后代及其基因运送到不同的栖息地。人们认为,来自邻近斑块的动物的遗传相似度高于更接近斑块的动物,但如果邻近斑块具有不同的栖息地特征,这些遗传相似模式可能无法维持。这个观点是我们理解适应和进化的基础,但它还没有在快速选择的影响方面得到充分的检验。这项研究应用新技术来测试即使不同环境特征的斑块比幼虫的传播距离更近,海洋动物的遗传特征是否也会发生变化。这项研究的重点是特拉华湾的东部牡蛎(Crassostrea virginica),以及它们承受水中盐分变化的能力。本研究将对不同盐度条件下河口牡蛎生存和生长的控制因素提供新的认识。这三名研究人员正在与资源管理者和利益相关者分享研究结果,以改善特拉华湾、切萨皮克湾和纽约的贝类恢复和牡蛎种群管理。一名康奈尔大学的博士后学者和马里兰大学的研究生在项目期间接受培训和指导。研究人员还与纽约和新泽西的教师培训项目合作,为中学生开发和传播有关牡蛎生态学的新课程材料。该项目将调查牡蛎的耐低盐度是否与幼虫扩散和定居后的生存力选择有关。在河口内的盐度区,基因流预计足够高,适应性分化不会由达尔文的多代过程产生。相反,每一代的循环生存力选择预计会在小空间尺度上产生该性状的空间变异。这种类型的世代内重复适应被称为表型-环境错配,并被假设为产生平衡多态性,但从未研究过单基因病例。该项目团队正在测试空间离散的选择模式,首先从不同的盐度区收集牡蛎,测量它们对低盐度的耐受性的变化,然后使用全基因组测序来测试这种特性与基因组变异之间的联系。实验低生育挑战可以在代内、基因组比较之前/之后识别由于强生存力选择而改变的DNA变异。候选基因和选择性中性对照基因位点将在来自相同盐度区域的幼虫、幼鱼和成虫样本中进行分析,以测试候选基因位点的变异与终生低盐度暴露之间的关联。将收集两年的环境数据,并将其添加到现有的长期数据集中,以绘制盐度变化图。观察到的低盐度耐受性的空间分布和与之相关的基因组变异提供了一个测试,可以明确地拒绝所有幼虫具有相似能力的普遍假设。如果不同亲代来源的幼虫在影响其在浮游生物中生存能力的性状上存在差异,那么表型-环境不匹配可能对种群连通性产生深远的影响。该项目提高了对牡蛎种群中幼虫扩散和招募变化机制的理解。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
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Daphne Munroe其他文献
Clam predator protection is effective and necessary for food production
- DOI:
10.1016/j.marpolbul.2015.09.042 - 发表时间:
2015-11-15 - 期刊:
- 影响因子:
- 作者:
Daphne Munroe;John Kraeuter;Brian Beal;Ken Chew;Mark Luckenbach;Charles P. Peterson - 通讯作者:
Charles P. Peterson
Evaluating the efficacy of nursery gear types for cultivating Atlantic surfclams (emSpisula solidissima/em)
评估用于养殖大西洋蛤蜊(Spisula solidissima)的育苗设备类型的功效
- DOI:
10.1016/j.aqrep.2022.101186 - 发表时间:
2022-08-01 - 期刊:
- 影响因子:3.700
- 作者:
Michael P. Acquafredda;Nathan Morris;Lisa Calvo;Michael De Luca;Daphne Munroe - 通讯作者:
Daphne Munroe
Interactive roles of temperature and food availability in predicting habitat suitability for marine invertebrates
温度和食物供应在预测海洋无脊椎动物栖息地适宜性中的相互作用
- DOI:
10.1016/j.ecss.2023.108515 - 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
Raymond Czaja;Brian Beal;Kyle Pepperman;Emmanuelle Pales Espinosa;Daphne Munroe;Robert M. Cerrato;Evan Busch;B. Allam - 通讯作者:
B. Allam
Evaluating the efficacy of nursery gear types for cultivating Atlantic surfclams (<em>Spisula solidissima</em>)
- DOI:
10.1016/j.aqrep.2022.101186 - 发表时间:
2022-08-01 - 期刊:
- 影响因子:
- 作者:
Michael P. Acquafredda;Nathan Morris;Lisa Calvo;Michael De Luca;Daphne Munroe - 通讯作者:
Daphne Munroe
Overlap between the Mid-Atlantic Bight Cold Pool and offshore wind lease areas
中大西洋湾冷池与海上风电租赁区重叠
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:3.3
- 作者:
Rebecca Horwitz;Travis N Miles;Daphne Munroe;Josh Kohut - 通讯作者:
Josh Kohut
Daphne Munroe的其他文献
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