Probing Non-Coding RNA in Live Cells by Convertible Tetrahedral DNA

核糖核酸（RNA）是在基因的编码、解码、调节和表达中的各种生物学作用中必不可少的聚合分子。非编码RNA（ncRNA）是不翻译成蛋白质的RNA分子。非编码RNA会导致癌症、自闭症和阿尔茨海默氏症等疾病。非编码RNA（non-coding RNA，nc RNA），如microRNA（microRNA，miRNA）和长链非编码RNA（long non-coding RNA，lncRNA），在多种生理和病理过程的调控中起着关键作用。然而，由于缺乏有效的工具来探测活细胞中的ncRNA，ncRNA的机制调节、亚细胞分布和作用模式仍然难以捉摸。PI将开发用于动态多基因分析的多光谱单分子条形码生物传感器。他们将建立一种可转换四面体DNA（CTED）生物传感器，沿着多光谱单分子传感（MSMS）系统，用于活细胞中的多重ncRNA检测。该奖项反映了NSF的法定使命，并通过使用该基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes. A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Non-coding RNAs contribute to diseases including cancers, autism, and Alzheimer's. Non-coding (nc) RNAs, such as microRNA (miRNA) and long non-coding RNA (lncRNA), play pivotal roles in the regulation of various physiological and pathological processes. Nonetheless, the mechanisticregulation, subcellular distribution, and mode of action of ncRNAs remain elusive due to a lack of effective tools for probing ncRNAs in live cells. The PI will develope a multispectral single molecule barcode biosensor for dynamic multigene analysis. They will establish a convertible tetrahedral DNA (CTED) biosensor along with a multispectral single molecule sensing (MSMS) system for multiplex ncRNA detection in live cells.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.