DISSECTING HORMONAL CROSSTALK DURING TOMATO FRUIT DEVELOPMENT

剖析番茄果实发育过程中的荷尔蒙串扰

• 批准号: 1818211
• 负责人: Paul Ode
• 金额: $ 48.37万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1818211&HistoricalAwards=false
• 关键词: DISSECTING; HORMONAL; CROSSTALK; DURING; TOMATO

Plants are essential for human life, providing food, fiber and a multitude of chemicals used in medicine and other aspects of our everyday life. Phytohormones are chemicals produced by plants to control plant physiology. This research applies recent technologies to simultaneously measure the levels of several types of phytohormones during plant development. In addition, levels of phytohormone-regulated gene expression are assessed and the resulting data are used to determine how these molecules work together to orchestrate plant growth. Results from this research provide essential knowledge for cultivating the future generation of advanced crop varieties with increased productivity and environmental stress tolerance. In addition, this research generates the first Hormonal Atlas of a plant species. This Hormonal Atlas, constructed with the assistance of college undergraduate students, will be available to the general public as well as to the scientific community, for purposes of education and research. Outreach activities are conducted to promote participation of K-12 and undergraduate students in science research.Understanding plant development is crucial for efforts of manipulating plant growth and yield. Phytohormones are essential regulators of plant growth and responses to the environment. This project uses recent technologies of high-throughput mass spectrometry and gene expression to generate quantitative outputs and define essential crosstalk relationships between the different hormonal pathways in tomato. The context of the study has a focus on fruit development. This research provides important insight on hormonal crosstalk and is likely to predict known as well as yet unidentified hormonal interactions. Such interactions may form the basis of future efforts for the development of synthetic hormonal networks with different functionalities. With the use of synthetic fine-tuning of plant growth, advanced crops with increased yield are possible. In addition, this project generates metabolomics, genetic, molecular and bioinformatics tools that will be available to the scientific community. These tools are expected to greatly contribute to the understanding of phytohormone regulation of other aspects of plant physiology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物是人类生活的必需品，提供食物、纤维和多种化学物质，用于医药和我们日常生活的其他方面。植物激素是植物产生的控制植物生理的化学物质。这项研究应用最新的技术来同时测量植物发育过程中几种类型的植物激素的水平。此外，还评估了植物激素调节的基因表达水平，并使用产生的数据来确定这些分子如何协同工作来协调植物的生长。这项研究结果为培育具有更高生产力和环境胁迫耐受性的下一代先进作物品种提供了必要的知识。此外，这项研究还生成了第一个植物物种的荷尔蒙图谱。这本荷尔蒙地图集是在大学本科生的帮助下制作的，将向普通公众和科学界开放，用于教育和研究。开展外展活动以促进K-12和本科生参与科学研究。了解植物发育对于控制植物生长和产量是至关重要的。植物激素是植物生长和对环境反应的重要调节因子。该项目使用最新的高通量质谱学和基因表达技术来产生定量输出，并确定番茄不同激素途径之间的基本串扰关系。这项研究的背景是水果的发展。这项研究提供了关于荷尔蒙串扰的重要见解，并有可能预测已知的和尚未确定的荷尔蒙相互作用。这种相互作用可能形成未来开发具有不同功能的合成荷尔蒙网络的基础。利用对植物生长的综合微调，可以获得增产的先进作物。此外，该项目还产生了可供科学界使用的代谢组学、遗传学、分子和生物信息学工具。这些工具预计将极大地有助于理解植物生理的其他方面的植物激素调节。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。