RAPID:Impact of unpredictable population-wide disturbances on evolutionary processes -- honey bees in Puerto Rico after Hurricane Maria

RAPID：不可预测的全种群干扰对进化过程的影响——飓风玛丽亚后波多黎各的蜜蜂

• 批准号: 1826729
• 负责人: Tugrul Giray
• 金额: $ 20万
• 依托单位: University of Puerto Rico-Rio Piedras
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1826729&HistoricalAwards=false
• 关键词: RAPID; Impact; unpredictable; population; wide

Large-scale, unpredictable disturbances such as storms can alter the environment, eliminating or evolutionarily changing plant and animal life, with potentially long-lasting effects. The goal of this project is to understand the role of foraging and aggressive behavior in determining hurricane survival in the honey bees of Puerto Rico. Africanized bees invaded Puerto Rico in the 1950's, initially creating highly aggressive populations as has occurred elsewhere. Since their introduction, the Africanized bees of Puerto Rico have uniquely become less aggressive over time, as evidenced by a wealth of pre-hurricane genetic and behavioral data. This project addresses the hypothesis that storms, such as Hurricane Maria that hit Puerto Rico on September 20, 2017, select against more aggressive colonies and favor bees that invest more in foraging and food hoarding. The researchers will test this hypothesis by sampling the remaining post-hurricane populations and comparing them with the pre-hurricane data. RAPID funding will provide the opportunity to study both genetic and behavioral traits of survivors and their offspring within the months immediately following the storm. The study of a population's response subsequent to major environmental and anthropogenic disturbances is of importance to the understanding of processes that shape natural and agricultural ecosystems. This project will also support Puerto Rican students to maintain research productivity in a lab severely damaged by the hurricane. The genomes of bees from post-Hurricane Maria wild populations and domestic populations will be sequenced and analyzed to determine signatures of selection or genetic bottleneck by comparing them with pre-hurricane genotypes. Polymorphic markers across the genome will be used to test a larger number (1500) of specimens over a greater geographic area (i.e. Puerto Rico) to examine evolutionary trends and processes. Comparisons of genetics, genomics and behavior of pre- and post-hurricane, feral and domesticated populations will test hypotheses on role of large disturbances such as hurricanes or anthropogenic influences on reducing aggressive behavior in Africanized honey bees. Untangling the effects of chance and selective processes on honey bee genomes may identify a novel and general role of environmental disturbance in the evolution of aggressive behavior in colonial organisms.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

风暴等大规模、不可预测的扰动可能会改变环境，消除或进化性地改变植物和动物的生命，并可能产生长期影响。 这个项目的目标是了解觅食和侵略行为在决定飓风生存的波多黎各蜜蜂的作用。非洲化的蜜蜂在20世纪50年代入侵波多黎各，最初创造了高度侵略性的种群，就像其他地方发生的那样。自引入以来，波多黎各的非洲化蜜蜂随着时间的推移变得不那么具有攻击性，这一点在飓风前的大量遗传和行为数据中得到了证明。该项目解决了这样一个假设，即风暴，如2017年9月20日袭击波多黎各的飓风玛丽亚，选择了更具侵略性的殖民地，并有利于在觅食和食物囤积方面投入更多的蜜蜂。研究人员将通过对飓风后剩余的种群进行采样并将其与飓风前的数据进行比较来验证这一假设。RAPID资金将提供机会，在风暴发生后的几个月内研究幸存者及其后代的遗传和行为特征。研究人口在重大环境和人为干扰之后的反应，对于理解形成自然和农业生态系统的过程具有重要意义。该项目还将支持波多黎各学生在被飓风严重破坏的实验室中保持研究生产力。来自飓风玛丽亚后野生种群和家养种群的蜜蜂的基因组将被测序和分析，以通过将它们与飓风前的基因型进行比较来确定选择或遗传瓶颈的特征。将使用跨基因组的多态性标记检测更大地理区域（即波多黎各）的大量（1500份）标本，以检查进化趋势和过程。 飓风前和飓风后，野生和驯化种群的遗传学，基因组学和行为的比较将测试大的干扰，如飓风或人为影响，减少非洲蜜蜂的攻击行为的作用的假设。 解开机会和选择过程对蜜蜂基因组的影响可能会发现一个新的和一般性的作用，环境干扰的进化中的侵略行为在殖民organis.This奖项反映了NSF的法定使命，并已被认为是值得通过评估使用基金会的智力价值和更广泛的影响审查标准的支持。

项目成果

Genomic regions influencing aggressive behavior in honey bees are defined by colony allele frequencies

• DOI: 10.1073/pnas.1922927117
• 发表时间: 2020-07-21
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Avalos, Arian;Fang, Miaoquan;Hudson, Matthew E.
• 通讯作者: Hudson, Matthew E.

Soybean aphid biotype 1 genome: Insights into the invasive biology and adaptive evolution of a major agricultural pest

• DOI: 10.1016/j.ibmb.2020.103334
• 发表时间: 2020-05-01
• 期刊: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 3.8
• 作者: Giordano, Rosanna;Donthu, Ravi Kiran;Zhan, Shuai
• 通讯作者: Zhan, Shuai