RII Track-2 FEC: Systems Genetics Studies on Rice Genomes for Analysis of Grain Yield and Quality Under Heat Stress

RII Track-2 FEC：用于分析热应激下谷物产量和品质的水稻基因组系统遗传学研究

• 批准号: 1826836
• 负责人: Andy Pereira
• 金额: $ 465.94万
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1826836&HistoricalAwards=false
• 关键词: RII; Track; FEC; Systems; Genetics

Non-technical DescriptionThe basic knowledge and understanding of how environmental stressors, such as high temperatures, affect cereal crop production is important for global food security. Rice, a model cereal plant and a major world staple crop, is grown in the southern United States, and is threatened by increased night temperatures that contribute to unstable production, resulting in lower grain yield and quality, which result in decreased market value. This project will identify individual rice lines that are tolerant to high nighttime temperatures and will use a variety of genetic and biochemical techniques to identify potential mechanisms that underlie the ability of these plants to be high temperature tolerant. These mechanisms will be tested in the plant using gene editing technologies to confirm that these mechanisms and thus the underling causative alleles are responsible for the plant's resistance to high nighttime temperatures. Knowing the actual mechanisms will aid breeders to develop new lines that can help decrease the risk of major crop losses due to high temperatures in the future. This project will be a collaborative effort among the University of Arkansas campuses at Fayetteville and Little Rock, Louisiana State University, and Oklahoma State University and will help develop the careers of six early career research faculty, train undergraduate and graduate students, and postdocs. In addition, the projects will build educational resources for STEM at the undergraduate level as well as K-12. Technical descriptionThe japonica rice subspecies is the basis of most US varieties currently in production and have been used in US breeding efforts with other rice introductions from all over the world to select varieties that are tolerant to high night temperature. In this project, a diverse collection of rice lines critical to U.S. production will be screened in the field, including environments differing in night temperature, and replicated under controlled greenhouse conditions, to identify heat tolerant genotypes with contributing genes and novel mechanisms that are of interest to use in improvement of rice and other cereals for their resilience to high night temperatures. The genetic changes or identification of alleles ascribed to the desired phenotypes will be characterized at multi-systems levels: the transcriptome, metabolome, proteome, and the physiological response. Information from these analyses will be integrated into gene regulatory networks that can provide a biological understanding of plant adaptations to the changing environment documented in independent genotypes. Wildtype or mutant alleles identified from the population studies will be validated by transformation and CRISPR/Cas9 mediated allele engineering, followed by phenotypic validation to identify alleles associated with heat tolerance, as proof of concept for use in cultivar development. This Research Infrastructure Improvement Track-2 Focused EPSCoR Collaboration (RII Track-2 FEC) project brings together complementary expertise comprising senior and junior faculty from the University of Arkansas campuses at Fayetteville and Little Rock, Louisiana State University, and Oklahoma State University, to address this complex challenge in an interdisciplinary manner. The participants include six early career faculty from different institutions, who will be integrated into the interdisciplinary program to develop expertise in cross-disciplinary research projects. The project will include training of postdocs, graduate and undergraduate students, participation of 10-12th grade students in STEM girl's leadership events, and of High School teachers and students for training in Plant Genetics and Physiology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

非技术性描述高温等环境压力因素如何影响谷类作物生产的基本知识和理解对全球粮食安全至关重要。水稻是一种典型的谷类植物，也是世界主要的主食作物，生长在美国南部，受到夜间气温升高的威胁，导致产量不稳定，导致粮食产量和质量下降，从而导致市场价值下降。 该项目将确定耐夜间高温的水稻品系，并将使用各种遗传和生物化学技术来确定这些植物耐高温能力的潜在机制。 这些机制将使用基因编辑技术在植物中进行测试，以确认这些机制以及潜在的致病等位基因负责植物对夜间高温的抗性。 了解实际的机制将有助于育种者开发新的品系，有助于降低未来高温造成主要作物损失的风险。 该项目将是阿肯色州大学费耶特维尔和小石城校区、路易斯安那州立大学和俄克拉荷马州州立大学之间的合作努力，并将帮助发展六名早期职业研究教师的职业生涯，培训本科生和研究生以及博士后。 此外，这些项目还将为本科和K-12的STEM建立教育资源。粳稻亚种是美国目前生产的大多数品种的基础，并已被用于美国育种工作，与来自世界各地的其他水稻引进，以选择耐夜间高温的品种。 在该项目中，将在田间筛选对美国生产至关重要的各种水稻品系，包括夜间温度不同的环境，并在受控温室条件下复制，以确定具有贡献基因和新机制的耐热基因型，这些基因和新机制可用于改善水稻和其他谷物对夜间高温的适应性。遗传变化或等位基因的鉴定归因于所需的表型将在多系统水平上表征：转录组、代谢组、蛋白质组和生理反应。从这些分析中获得的信息将被整合到基因调控网络中，这些网络可以提供对独立基因型中记录的植物适应不断变化的环境的生物学理解。 从群体研究中鉴定的野生型或突变等位基因将通过转化和CRISPR/Cas9介导的等位基因工程进行验证，然后进行表型验证以鉴定与耐热性相关的等位基因，作为用于栽培品种开发的概念证明。 这一研究基础设施改善轨道-2重点EPSCoR合作（RII轨道-2 FEC）项目汇集了互补的专业知识，包括来自阿肯色州费耶特维尔和小石城，路易斯安那州州立大学和俄克拉荷马州州立大学校园的高级和初级教师，以跨学科的方式解决这一复杂的挑战。 参与者包括来自不同机构的六名早期职业教师，他们将被纳入跨学科计划，以发展跨学科研究项目的专业知识。该项目将包括对博士后、研究生和本科生的培训，10- 12年级学生参加STEM女孩领导活动，以及高中教师和学生参加植物遗传学和生理学培训。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

MicroRNA profiles in Sorghum exposed to individual drought or heat or their combination

• DOI: 10.1007/s13562-021-00747-0
• 发表时间: 2021-11-26
• 期刊: JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
• 影响因子: 1.9
• 作者: Puli，Chandra Obul Reddy;Zheng，Yun;Sunkar，Ramanjulu
• 通讯作者: Sunkar，Ramanjulu

Physiological and transcriptional responses to low-temperature stress in rice genotypes at the reproductive stage

• DOI: 10.1080/15592324.2019.1581557
• 发表时间: 2019-04-03
• 期刊: PLANT SIGNALING & BEHAVIOR
• 影响因子: 2.9
• 作者: Moraes de Freitas, Gabriela Peres;Basu, Supratim;Pereira, Andy
• 通讯作者: Pereira, Andy

Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane

• DOI: 10.1007/s11103-019-00856-4
• 发表时间: 2019-03
• 期刊: Plant Molecular Biology
• 影响因子: 5.1
• 作者: Yang Zhao;J. Y. Kim;R. Karan;J. Jung;B. Pathak;Bruce Williamson;Baskaran Kannan;Duoduo Wang;Chunyang Fan;Wenjin Yu;Shujie Dong;V. Srivastava;F. Altpeter

Utility of I-SceI and CCR5-ZFN nucleases in excising selectable marker genes from transgenic plants

I-SceI 和 CCR5-ZFN 核酸酶在从转基因植物中切除选择性标记基因中的用途

• DOI: 10.1186/s13104-019-4304-2
• 发表时间: 2019
• 期刊: BMC Research Notes
• 影响因子: 1.8
• 作者: Pathak, Bhuvan P.;Pruett, Eliott;Guan, Huazhong;Srivastava, Vibha
• 通讯作者: Srivastava, Vibha

Identification and mapping of a novel resistance gene to the rice pathogen, Cercospora janseana

• DOI: 10.1007/s00122-021-03821-2
• 发表时间: 2021-04-07
• 期刊: THEORETICAL AND APPLIED GENETICS
• 影响因子: 5.4
• 作者: Addison, Christopher K.;Angira, Brijesh;Famoso, Adam N.
• 通讯作者: Famoso, Adam N.