CAREER: Tackling the Solvent-Stabilizer Co-contamination by Propanotrophic Bacteria with Catalytically Versatile Di-iron Monooxygenases

职业生涯:利用催化多功能二铁单加氧酶解决丙营养菌引起的溶剂稳定剂双重污染

基本信息

  • 批准号:
    1846945
  • 负责人:
  • 金额:
    $ 50万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-06-01 至 2025-05-31
  • 项目状态:
    未结题

项目摘要

Thousands of aquifers are contaminated with mixtures of chlorinated aliphatic hydrocarbon (CAH) solvents and stabilizers like 1,4-dioxane. To date, there is no single remediation strategy capable of treating both types of pollutants simultaneously. To tackle the problem, an innovative biological treatment will be developed that employs bacteria growing on propane that are capable of simultaneously degrading both CAHs and 1,4-dioxane. Development of this approach requires the isolation of a diverse collection of bacterial strains capable of effective treatment under various field conditions. This novel remediation strategy is potentially transformative in its precision targeting for specific contaminated sites. An added benefit of this approach results from it being less disruptive to the environment compared to other available remedial strategies. Through laboratory experimentation and hands-on training with stakeholders, the investigator will develop the next generation of environmental remediation professionals. Recruitment of students from diverse backgrounds will broaden participation in these fields.Bioremediation is frequently a preferred groundwater contamination treatment method because it uses a natural approach to degrade pollutants at relatively low cost. However, the success of this approach is highly dependent on the specific physiochemical characteristics and the indigenous microbiota of a given site, resulting in site-specific solutions with narrow applicability. To increase the effectiveness of bioremediation, the PI will establish a collection of propanotrophic isolates and consortia exhibiting robust degradation capabilities and environmental adaptabilities. This strategy relies on the synergy of conventional cultivation approaches and state-of-the-art biotechnologies such as metagenomics and single-cell genomic analysis to advance understanding of cometabolic biodegradation at the molecular, cellular, and microbial community levels. This research focuses on propane-inducible soluble di-iron monooxygenases (SDIMOs) as versatile biocatalysts, uniquely capable of degrading a variety of chlorinated solvents and 1,4-dioxane. Novel SDIMOs will be identified and their catalytic capacities and kinetics will be characterized in heterologous expression clones and knockout mutants. These insights will be used to refine the categories of SDIMOs to enable the design and interpretation of omics and other molecular biological tools to exploit the metabolic interactions among propanotrophic consortia obtained from different polluted habitats. Finally, bench-scale microcosm assays will be used to evaluate and compare the feasibility and reliability of conventional and diagnosis-based bioremediation strategies to advance their in situ applications. This research will be actively integrated with a core educational goal to cultivate the next generation of remediation professionals needed to address growing demand in the historically polluted New York-New Jersey-Connecticut tristate area. Taking advantage of the sizeable remediation industry in this area, the PI will engage students of all levels with a diverse range of on- and off-campus activities, such as guest lectures with environmental experts, professional workshops with alumni in the field, and University-sponsored research projects, as well as internships and networking opportunities with private and non-profit remediation organizations in the region. These experiences will give students critical access to applied research and training in the field, and foster long-lasting connections that are key catalysts for career success. Further, the PI will attract motivated students from underrepresented groups at two-year colleges and local high schools to take part in scientific seminars, laboratory tours, and part-time research opportunities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
数以千计的含水层被氯化脂肪烃(CAH)溶剂和1,4-二恶烷等稳定剂的混合物污染。到目前为止,还没有一个单一的补救战略能够同时处理这两类污染物。为了解决这个问题,将开发一种创新的生物处理方法,使用在丙烷上生长的能够同时降解CAHs和1,4-二恶烷的细菌。这种方法的发展需要分离出能够在不同田间条件下进行有效治疗的各种细菌菌株。这一新的补救策略在其针对特定受污染地点的精确定位方面具有潜在的变革性。这种方法的另一个好处是,与其他可用的补救策略相比,它对环境的破坏较小。通过实验室实验和与利益相关者的实践培训,调查员将培养下一代环境修复专业人员。招收不同背景的学生将扩大在这些领域的参与。生物修复通常是一种首选的地下水污染处理方法,因为它使用一种自然的方法以相对较低的成本降解污染物。然而,这种方法的成功高度依赖于特定地点的特定物理化学特征和本地微生物区系,导致特定地点的解决方案适用性很窄。为了提高生物修复的有效性,PI将建立一系列具有强大的降解能力和环境适应能力的丙酸营养菌株和联合体。这一战略依赖于传统培养方法和最新生物技术(如元基因组学和单细胞基因组分析)的协同作用,以在分子、细胞和微生物群落水平上促进对共代谢生物降解的理解。本研究致力于丙烷诱导的可溶性二铁单加氧酶(SDIMO)作为一种多功能的生物催化剂,能够降解多种氯化溶剂和1,4-二恶烷。新的SDIMO将被鉴定,它们的催化能力和动力学将在异源表达克隆和敲除突变体中得到表征。这些见解将被用来完善SDIMO的类别,使组学和其他分子生物学工具的设计和解释能够利用从不同受污染的生境获得的丙素营养联合体之间的代谢相互作用。最后,将使用实验室规模的微观世界分析来评估和比较常规和基于诊断的生物修复策略的可行性和可靠性,以促进其在现场的应用。这项研究将与培养下一代补救专业人员的核心教育目标积极结合,以满足历史上受到污染的纽约州-新泽西州-康涅狄格州三州地区日益增长的需求。利用这一领域庞大的补救行业,PI将通过各种校内外活动吸引各级学生,例如与环境专家的客座讲座、与该领域校友的专业研讨会、大学赞助的研究项目,以及与该地区私人和非营利性补救组织的实习和网络机会。这些经验将使学生获得关键的应用研究和该领域的培训,并培养长期持久的联系,这是职业成功的关键催化剂。此外,PI将吸引来自两年制大学和当地高中代表不足群体的有积极性的学生参加科学研讨会、实验室参观和兼职研究机会。该奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sequential anaerobic and aerobic bioaugmentation for commingled groundwater contamination of trichloroethene and 1,4-dioxane
  • DOI:
    10.1016/j.scitotenv.2021.145118
  • 发表时间:
    2021-02-19
  • 期刊:
  • 影响因子:
    9.8
  • 作者:
    Li, Fei;Deng, Daiyong;Li, Mengyan
  • 通讯作者:
    Li, Mengyan
Effective removal of trace 1,4-dioxane by biological treatments augmented with propanotrophic single culture versus synthetic consortium
通过生物处理有效去除痕量 1,4-二恶烷,并辅以丙营养单一培养物与合成联合培养物
  • DOI:
    10.1016/j.hazadv.2023.100246
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Li, Fei;Deng, Daiyong;Wadden, Andrew;Parvis, Patricia;Cutt, Diana;Li, Mengyan
  • 通讯作者:
    Li, Mengyan
Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range
  • DOI:
    10.1021/acs.est.9b05671
  • 发表时间:
    2020-02-04
  • 期刊:
  • 影响因子:
    11.4
  • 作者:
    Li, Fei;Deng, Daiyong;Li, Mengyan
  • 通讯作者:
    Li, Mengyan
Emerging investigator series: environment-specific auxiliary substrates tailored for effective cometabolic bioremediation of 1,4-dioxane
新兴研究者系列:针对 1,4-二恶烷的有效共代谢生物修复而定制的环境特异性辅助底物
Complete Genome Sequence of Azoarcus sp. Strain DD4, a Gram-Negative Propanotroph That Degrades 1,4-Dioxane and 1,1-Dichloroethylene
  • DOI:
    10.1128/mra.00775-19
  • 发表时间:
    2019-08-01
  • 期刊:
  • 影响因子:
    0.8
  • 作者:
    Deng, Daiyong;Li, Eel;Li, Mengyan
  • 通讯作者:
    Li, Mengyan
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Mengyan Li其他文献

Electrospun Blends of Natural and Synthetic Polymers as Scaffolds for Tissue Engineering
天然和合成聚合物的静电纺丝混合物作为组织工程的支架
Histone H1 deamidation facilitates chromatin relaxation for DNA repair
组蛋白 H1 脱酰胺作用促进染色质松弛以进行 DNA 修复
  • DOI:
    10.1038/s41586-025-08835-0
  • 发表时间:
    2025-04-16
  • 期刊:
  • 影响因子:
    48.500
  • 作者:
    Yuan Tian;Tingting Feng;Jun Zhang;Qingren Meng;Wenxin Zhan;Ming Tang;Chaohua Liu;Mengyan Li;Wenhui Tao;Yuxin Shu;Yu Zhang;Feng Chen;Shunichi Takeda;Qian Zhu;Xiaopeng Lu;Wei-Guo Zhu
  • 通讯作者:
    Wei-Guo Zhu
Designing Intelligent Polymeric Scaffolds for Tissue Engineering: Blending and Co-Electrospinning Synthetic and Natural Polymers
设计组织工程智能聚合物支架:合成和天然聚合物的共混和共静电纺丝
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    P. Lelkes;Mengyan Li;A. Perets;M. Mondrinos;Yi Guo;Xuesi Chen;A. MacDiarmid;F. Ko;C. Finck;Yen Wei
  • 通讯作者:
    Yen Wei
Cannabidiol-loaded hydrogel microneedle patches inhibit TRIM14/TRAF3/ NF-κB axis for the treatment of psoriasis
载大麻二酚水凝胶微针贴剂通过抑制TRIM14/TRAF3/NF-κB轴治疗银屑病
  • DOI:
    10.1016/j.ijbiomac.2025.140825
  • 发表时间:
    2025-04-01
  • 期刊:
  • 影响因子:
    8.500
  • 作者:
    Mengyan Li;Die Li;Yu Zhang;Juan Wang;Shenglan Wang;Hao Zhao;Hanying Wang;Xiaofeng Zeng;Dan Xu;Lechun Lyu
  • 通讯作者:
    Lechun Lyu
Experimental study on the prevention and control of soil and water loss in Karst rocky mountain areas with PAM solution
  • DOI:
    10.1007/s13146-025-01137-6
  • 发表时间:
    2025-06-30
  • 期刊:
  • 影响因子:
    1.300
  • 作者:
    Zhanfei Gu;Pengxiang Jia;Mengyan Li;Hailong Wei
  • 通讯作者:
    Hailong Wei

Mengyan Li的其他文献

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{{ truncateString('Mengyan Li', 18)}}的其他基金

Collaborative Research: Directed Enzyme Evolution Accelerated by Machine Learning for Enhancing the Biodegradation of Emerging Contaminants
合作研究:机器学习加速定向酶进化,增强新兴污染物的生物降解
  • 批准号:
    2203616
  • 财政年份:
    2022
  • 资助金额:
    $ 50万
  • 项目类别:
    Continuing Grant
INFEWS: US-CHINA: Biochar-Enabled Biologically Active Filtration System for Sustainable Water Management in Rice Agriculture
INFEWS:中美:生物炭生物活性过滤系统用于水稻农业可持续水管理
  • 批准号:
    1903597
  • 财政年份:
    2019
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant

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