Collaborative Research: Camponotine Ants and their Little Helpers: Phylogenomics of a Hyperdiverse Insect Clade and its Bacterial Endosymbionts (CAnBE)

合作研究：Camponotine 蚂蚁和它们的小帮手：超多样化昆虫进化枝及其细菌内共生体的系统基因组学 (CAnBE)

• 批准号: 1856539
• 负责人: Philip Ward
• 金额: $ 31.86万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1856539&HistoricalAwards=false
• 关键词: Collaborative; Research; Camponotine; Ants; their

Recent years have seen an explosion of curiosity and interest in the microbial associates of humans, especially symbiotic associations with gut microbiota that convey benefits to human health. Such nutritional symbioses occur frequently across many different organisms, including primates, plants, and insects. This research will investigate the joint evolutionary history of a highly successful and diverse group of insects, carpenter ants and their relatives, and the bacteria Blochmannia. Carpenter ants are the most diverse and abundant group of ants in the world - most kids have encountered them while playing outside. All carpenter ants carry Blochmannia bacteria inside their gut cells, making the bacterium an endosymbiont. Both organisms rely on one another to survive. Evidence indicates that carpenter ants and Blochmannia have maintained a close nutritional partnership for tens of millions of years. Obligate symbiotic associations among different kinds of organisms can profoundly impact the nature and tempo of the evolution of both partners, and microbial endosymbionts have been suggested to have played important roles in the diversification of some insect groups. This research will determine how the carpenter ants and their relatives evolved alongside Blochmannia. The findings will improve our understanding of how symbioses arise and are maintained in animals, with potential implications for the evolution of human-associated microbial systems. Moreover, the project will emphasize public education in ant biodiversity and microbial symbioses, and the training of early-career scientists.This research will infer the phylogeny, divergence times, and biogeographic history of ants in the tribe Camponotini, and use this comparative framework to advance systematic and co-evolutionary studies of their endosymbiotic bacteria Blochmannia. Like other obligate endosymbionts, Blochmannia exhibits substantial genome reduction compared to free-living bacteria. Genome evolution and functional variation in Blochmannia will be evaluated across a phylogenetically representative selection of camponotine ants with contrasting morphological and ecological attributes. By jointly elucidating the ant and endosymbiont evolutionary histories, the project will address such questions as: (1) What are the major features of Blochmannia genome evolution across the Camponotini clade? (2) What processes shape rates and patterns of Blochmannia sequence evolution? (3) Is Blochmannia gene content variation associated with particular camponotine host attributes? By linking phylogenetic and trait data on the ants to functional genomic properties of the bacteria we will gain an understanding of the selective forces and constraints underlying this remarkable symbiosis. A detailed phylogeny of the camponotine ants will also create a strong foundation for much-needed monographic work on this taxonomically neglected group, and allow the development of more effective identification tools.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

近年来，人们对人类微生物的好奇心和兴趣激增，特别是与肠道微生物群的共生关系，这些微生物群对人类健康有益。这种营养共生现象经常发生在许多不同的生物体中，包括灵长类动物、植物和昆虫。这项研究将调查一个非常成功和多样化的昆虫，木蚁及其亲属和细菌Blochmannia的联合进化史。木匠蚁是世界上种类最多、数量最多的蚂蚁，大多数孩子在户外玩耍时都会遇到它们。所有的木蚁都在肠道细胞内携带Blochmannia细菌，使细菌成为内共生体。这两种生物都依赖彼此生存。有证据表明，木蚁和Blochmannia已经保持了数千万年的密切营养伙伴关系。不同种类生物之间的专性共生关系可以深刻地影响双方进化的性质和克里思，微生物内共生体已经被认为在一些昆虫类群的多样化中发挥了重要作用。这项研究将确定木蚁及其亲属是如何与Blochmannia一起进化的。这些发现将提高我们对共生如何在动物中产生和维持的理解，并对人类相关微生物系统的进化产生潜在影响。此外，该项目将强调蚂蚁生物多样性和微生物共生的公众教育，以及早期职业科学家的培训。本研究将推断Camponotini部落蚂蚁的繁殖，分歧时间和地理历史，并使用这个比较框架来推进其内共生细菌Blochmannia的系统和共同进化研究。与其他专性内共生体一样，与自由生活的细菌相比，Blochmannia表现出大量的基因组减少。在Blochmannia的基因组进化和功能变异将评估整个camponotine蚂蚁的形态和生态属性形成鲜明对比的遗传代表性选择。通过共同阐明蚂蚁和内共生体的进化历史，该项目将解决这样的问题：（1）在Camponotini分支中，Blochmannia基因组进化的主要特征是什么？(2)什么样的过程塑造了Blochmannia层序演化的速率和模式？(3)是Blochmannia基因含量的变化与特定camponotine主机属性？通过将蚂蚁的系统发育和性状数据与细菌的功能基因组特性联系起来，我们将了解这种显着的共生关系背后的选择力和限制。一个详细的camponotine蚂蚁的进化史也将创造一个强大的基础，急需专题工作对这个分类学上被忽视的群体，并允许更有效的识别工具的发展。这个奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

Ant phylogenomics reveals a natural selection hotspot preceding the origin of complex eusociality

• DOI: 10.1016/j.cub.2022.05.001
• 发表时间: 2022-07-11
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Romiguier, Jonathan;Borowiec, Marek L.;Keller, Laurent
• 通讯作者: Keller, Laurent

Taxonomy in the phylogenomic era: species boundaries and phylogenetic relationships among North American ants of the Crematogaster scutellaris group (Formicidae: Hymenoptera)

系统发育时代的分类学：北美火蚁类（蚁科：膜翅目）的物种边界和系统发育关系

• DOI: 10.1093/zoolinnean/zlab047
• 发表时间: 2022
• 期刊: Zoological Journal of the Linnean Society
• 影响因子: 2.8
• 作者: Ward, Philip S.;Blaimer, Bonnie B.
• 通讯作者: Blaimer, Bonnie B.

Biogeography and evolution of social parasitism in Australian Myrmecia bulldog ants revealed by phylogenomics

• DOI: 10.1016/j.ympev.2023.107825
• 发表时间: 2023-06-08
• 期刊: MOLECULAR PHYLOGENETICS AND EVOLUTION
• 影响因子: 4.1
• 作者: Mera-Rodriguez，Daniela;Jourdan，Herve;Rabeling，Christian
• 通讯作者: Rabeling，Christian

Grappling with homoplasy: taxonomic refinements and reassignments in the ant genera Camponotus and Colobopsis (Hymenoptera: Formicidae)

解决同质性：弓背蚁属和疣蚁属的分类学改进和重新分配（膜翅目：蚁科）

• DOI: 10.3897/asp.79.e66978
• 发表时间: 2021
• 期刊: Arthropod Systematics & Phylogeny
• 影响因子: 1.8
• 作者: Ward, Philip S.;Boudinot, Brendon E.
• 通讯作者: Boudinot, Brendon E.