Collaborative Research: Convergent evolution and diversification of the crab body plan over 200 million years

合作研究：2亿年来蟹体计划的趋同进化和多样化

• 批准号: 1856679
• 负责人: JAVIER ORTEGA-HERNANDEZ
• 金额: $ 48.22万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1856679&HistoricalAwards=false
• 关键词: Collaborative; Research; Convergent; evolution; diversification

This project addresses a fundamental question in the study of life: can we predict the evolutionary success of animals based on their forms? Crabs are one of the most iconic groups of invertebrates, playing an integral role in aquarium trade, fisheries, and aquaculture, and are celebrated in cultures around the world through festivals and parades. This research aims to build the tree of life for all known living and fossil crab families, to investigate how these animals have changed over 200 million years of history. The typical body form that characterizes crabs has evolved separately several times from lobster-like ancestors, and also has been lost several times in different groups of crabs. The new crab tree of life will reveal whether the independent gains and losses of the crab shape predict the immense biodiversity and ecological versatility of the group. The inspiring diversity of crab form and function will engage high school students through museum activities, and undergraduate students in primary research and classroom modules incentivizing critical thinking. Fieldwork will be conducted in Panama, where a training course in tropical crab taxonomy will immerse US and Latin American scientists in the techniques used to expand and investigate the crab tree of life.The acquisition of a crab-like body form has evolved four to five times among decapod crustaceans, and has been proposed as a contributing factor to the immense diversification and ecological success within the infraorders Brachyura (7000 species of true crabs), and Anomura (2500 species including porcelain crabs, hermit crabs, and king crabs). The objectives of this project are: (1) To construct a well-supported backbone phylogeny for false and true crabs using genomic (extant) and morphological (extant and fossil) data. (2) To employ fossils as age calibrations to construct an evolutionary timeline; this will serve as the foundation for inferring the pattern and geological timing of crab body plan evolution. (3) To test if the rate of diversification has increased in crab-like lineages relative to their sister groups, and likewise has decreased when the crab-like body was secondarily lost. The researchers hypothesize that at least some traits comprise a possible key innovation. These objectives will be achieved by sequencing 500 crab species using target enrichment methods, morphological coding and imaging of living and fossil taxa (including representatives of all crab families), and the deployment of cutting-edge strategies for divergence time analysis and phylogenetic comparative methods.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个项目解决了生命研究中的一个基本问题：我们能根据动物的形态预测它们的进化成功吗？螃蟹是无脊椎动物中最具代表性的群体之一，在水族馆贸易，渔业和水产养殖中发挥着不可或缺的作用，并通过节日和游行在世界各地的文化中庆祝。这项研究旨在为所有已知的活的和化石螃蟹家族建立生命树，以调查这些动物在2亿年的历史中是如何变化的。蟹类的典型体型已经从龙虾类的祖先进化了好几次，也在不同的蟹类中消失了好几次。新的螃蟹生命树将揭示螃蟹形状的独立收益和损失是否预测了该群体巨大的生物多样性和生态多样性。螃蟹形态和功能的多样性将通过博物馆活动吸引高中生，并将本科生吸引到初级研究和课堂模块中，以激励批判性思维。实地考察将在巴拿马进行，在那里，一个热带螃蟹分类学的培训课程将使美国和拉丁美洲的科学家沉浸在用于扩展和调查螃蟹生命树的技术中。在十足类甲壳动物中，获得螃蟹样的身体形式已经进化了四到五次，并被认为是短尾亚目的巨大多样性和生态成功的一个贡献因素（真蟹7000种）、异尾蟹（瓷蟹、寄居蟹、帝王蟹等2500种）。本研究的主要目的是：（1）利用现存的基因组和化石的形态学资料，构建一个支持性较强的伪蟹和真蟹的骨干系统发育。(2)以化石作为年龄刻度，建立一个演化的时间轴;这将作为推断螃蟹体平面演化模式和地质时间的基础。(3)为了检验在蟹类谱系中，相对于它们的姐妹群体，多样化的速度是否增加了，而当蟹类身体第二次消失时，多样化的速度是否降低了。研究人员假设，至少有一些特征构成了可能的关键创新。这些目标将通过使用目标富集方法、形态编码和活的和化石分类群成像对500种螃蟹进行测序来实现（包括所有螃蟹家族的代表），和切割的部署-该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值进行评估来支持和更广泛的影响审查标准。

项目成果

The oldest dairoidid crab (Decapoda, Brachyura, Parthenopoidea) from the Eocene of Spain

• DOI: 10.1002/spp2.1494
• 发表时间: 2023-05
• 期刊: Papers in Palaeontology
• 影响因子: 2.3
• 作者: F. Ferratges;J. Luque;J. L. Domínguez;À. Ossó;M. Aurell;S. Zamora

How to become a crab: Phenotypic constraints on a recurring body plan

• DOI: 10.1002/bies.202100020
• 发表时间: 2020-12
• 期刊: BioEssays
• 影响因子: 4
• 作者: Joanna M. Wolfe;J. Luque;H. Bracken-Grissom

Convergent Adaptation of True Crabs (Decapoda: Brachyura) to a Gradient of Terrestrial Environments

真正的螃蟹（十足目：Brachyura）对陆地环境梯度的趋同适应

• DOI: 10.1093/sysbio/syad066
• 发表时间: 2023
• 期刊: Systematic Biology
• 影响因子: 6.5
• 作者: Wolfe, Joanna M.;Ballou, Lauren;Luque, Javier;Watson-Zink, Victoria M.;Ahyong, Shane T.;Barido-Sottani, Joëlle;Chan, Tin-Yam;Chu, Ka Hou;Crandall, Keith A.;Daniels, Savel R.
• 通讯作者: Daniels, Savel R.

Major Revisions in Pancrustacean Phylogeny and Evidence of Sensitivity to Taxon Sampling

甲壳动物系统发育的重大修订和对分类单元采样敏感性的证据

• DOI: 10.1093/molbev/msad175
• 发表时间: 2023
• 期刊: Molecular Biology and Evolution
• 影响因子: 10.7
• 作者: Bernot, James P;Owen, Christopher L;Wolfe, Joanna M;Meland, Kenneth;Olesen, Jørgen;Crandall, Keith A
• 通讯作者: Crandall, Keith A