Catalyst Award: Assessing the Role of Natural Compounds on Mechanisms of Apoptosis in RT4-D2P6T cells

催化剂奖：评估天然化合物对 RT4-D2P6T 细胞凋亡机制的作用

• 批准号: 1901040
• 负责人: Rosalyn Reid
• 金额: $ 14.99万
• 依托单位: Johnson C. Smith University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1901040&HistoricalAwards=false
• 关键词: Catalyst; Award; Assessing; Role; Natural

Catalyst Projects provide support for Historically Black Colleges and Universities to work towards establishing research capacity of faculty to strengthen science, technology, engineering and mathematics undergraduate education and research. It is expected that the award will further the faculty member's research capability, improve research and teaching at the institution, and involve undergraduate students in research experiences. This project at Johnson C. Smith University will explore the ability of natural compounds to permanently inhibit or prevent the progression of cell death via apoptosis in neuronal cells. The project will increase the exposure and engagement of underrepresented students in hands-on, inquiry-based research in the biology of aging and prepare them for graduate and pre-professional programs in STEM.This project forwards the trajectory to find natural compounds that impact molecular switches that may permanently slow or stop the progression of apoptosis in neuronal cells. The central hypothesis is that flavonoids may inhibit, block or prevent the progression of apoptosis in HT22 neuronal cells. Cell death via apoptosis leads to the activation of molecules such as caspases which have been shown to induce apoptosis. Although studies show that flavonoids are able to inhibit apoptosis in human normal astrocytes, few studies have focused on this aspect of flavonoid bioactivity or the molecular targets impacted in this role. This project is an in-vitro study with the primary goal of assessing the effects of flavonoids on neuronal cell death via apoptosis, an issue that is critically associated to the fields of systems biology, neurobiology, and nutrition science. The neuronal cell line HT22, will be used in this study for the purpose of assessing the impact of quercetin and macluraxanthone on gene and protein expression of molecular targets (caspases 3, 8, 9; iROS; iNOS) and to measure the impact of antioxidant compounds on the rate of cell death via apoptosis in vitro. The findings from this study will increase knowledge of inhibition of cell death via apoptosis in neuronal cells.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

催化剂项目为历史上的黑人学院和大学提供支持，努力建立教师的研究能力，以加强科学，技术，工程和数学本科教育和研究。预计该奖项将进一步提高教师的研究能力，改善研究和教学机构，并参与本科生的研究经验。这个项目在约翰逊C。史密斯大学将探索天然化合物通过神经元细胞凋亡永久抑制或阻止细胞死亡进展的能力。该项目将增加接触和参与不足的学生在动手，调查为基础的研究在衰老生物学，并准备他们在干研究生和预科课程。该项目的前进轨迹，以找到影响分子开关，可能永久减缓或停止神经元细胞凋亡的进展天然化合物。核心假设是类黄酮可以抑制、阻断或阻止HT 22神经元细胞凋亡的进展。通过细胞凋亡的细胞死亡导致分子如半胱天冬酶的活化，半胱天冬酶已被证明可诱导细胞凋亡。虽然研究表明黄酮类化合物能够抑制人类正常星形胶质细胞的凋亡，但很少有研究集中在黄酮类化合物生物活性的这一方面或影响这一作用的分子靶点。该项目是一项体外研究，主要目标是评估类黄酮对通过细胞凋亡的神经细胞死亡的影响，这是一个与系统生物学，神经生物学和营养科学领域密切相关的问题。 本研究中将使用神经元细胞系HT 22，以评估槲皮素和macluraxanthone对分子靶点（半胱天冬酶3、8、9; iROS; iNOS）的基因和蛋白表达的影响，并测量抗氧化化合物对体外细胞凋亡导致的细胞死亡率的影响。这项研究的发现将增加通过神经元细胞凋亡抑制细胞死亡的知识。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。