NSF Postdoctoral Fellowship in Biology FY 2019: Untangling the evolutionary and ecological mechanisms that shape gut microbiomes: Integrating behavior, microbes, and ecology

2019 财年 NSF 生物学博士后奖学金：解开塑造肠道微生物群的进化和生态机制：整合行为、微生物和生态

• 批准号: 1906416
• 负责人: Lydia Greene
• 金额: $ 13.8万
• 依托单位: Greene, Lydia Kathryn
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1906416&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; FY

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2019, Research Using Biological Collections. The fellowship supports research and training of the fellow that will utilize biological collections in innovative ways. The gut microbiome is the community of bacteria that lives in an animal's gastrointestinal tracts. The microbiome helps shape the lives of their hosts across days, seasons, lifetimes, and generations. Captive wildlife, like those housed at zoos or research facilities, provide the opportunity to explore how the gut microbiome is controlled; however, compared to laboratory models, they remain untapped resources. The Biological Collections at the Duke Lemur Center (DLC), including a living primate colony and a tissue bank, are well suited to microbiome research. The fellow will use these biological collections to test how diet, and the specific consumption of foliage, shape the gut microbiome across leaf eaters, fruit eaters, and omnivores in primates. Ultimately, the value of this project is twofold: (1) It will create knowledge about what controls the gut microbiome in primates, with potential applied value to human healthcare and wildlife conservation; and (2) Through diverse sponsorship, the project will stimulate dialogue across scientific disciplines and will lead to the development of resources for the research community.The project will use Biological Collections to test the hypothesis that, compared to other feeding strategies, folivory more strongly drives host-microbiome coadaptation. In three studies, the fellow will probe how foliage consumption differentially drives gut-microbiome dynamics across folivorous, omnivorous, and frugivorous lemurs. Whereas the first study uses banked samples to compare microbiota along the species' gastrointestinal tracts, the second and third studies use the living colony, with study two asking how free-choice foraging shapes feeding and microbial ecology across seasonal scales, and study three using dietary manipulations to determine how cellulose and tannins regulate communities across daily scales. In addition to standard microbiome methods, during training residencies with sponsors Clayton and Eley, the fellow will develop metrics of dietary reconstruction from fecal samples via chloroplast sequencing, isotopic and wax-alkane analysis, and of microbiome dynamics via anaerobic and experimental culturing. The project is expected to yield research and review-style publications, and methodological descriptions. The fellow will mentor undergraduates throughout and work with the Duke Lemur Center's Education and Communication departments to create new materials for school children, the general public, and the social-media audience.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项行动资助了2019财年NSF生物学博士后研究奖学金，使用生物收集的研究。该研究金支持研究员的研究和培训，以创新的方式利用生物收藏。肠道微生物组是生活在动物胃肠道中的细菌群落。微生物组有助于塑造宿主在不同日子、季节、一生和几代人中的生活。圈养野生动物，如动物园或研究机构中的野生动物，提供了探索肠道微生物组如何控制的机会;然而，与实验室模型相比，它们仍然是未开发的资源。杜克狐猴中心（DLC）的生物收藏品，包括一个活的灵长类动物群落和一个组织库，非常适合微生物组研究。该研究员将使用这些生物收集来测试饮食和树叶的特定消费如何塑造灵长类动物中食叶动物，食果动物和杂食动物的肠道微生物组。最终，该项目的价值是双重的：（1）它将创造关于控制灵长类动物肠道微生物组的知识，对人类医疗保健和野生动物保护具有潜在的应用价值;（2）通过多种赞助方式，该项目将促进跨学科的对话，并将为研究界开发资源。该项目将利用生物标本来测试这一假说认为，与其他喂养策略相比，食叶性更强烈地驱动宿主-微生物组的共适应。在三项研究中，该研究员将探讨树叶消费如何不同地驱动食叶，杂食和食果狐猴的肠道微生物组动态。第一项研究使用库存样本来比较沿着物种胃肠道的微生物群，第二项和第三项研究使用活体菌落，研究二询问自由选择觅食如何在季节尺度上塑造摄食和微生物生态，研究三使用饮食操作来确定纤维素和单宁如何在日常尺度上调节群落。除了标准的微生物组方法，在与赞助商克莱顿和埃利的培训实习期间，该研究员将通过叶绿体测序，同位素和石蜡烷烃分析，以及通过厌氧和实验培养的微生物组动力学，从粪便样本中开发饮食重建指标。预计该项目将产生研究和审查风格的出版物以及方法说明。该研究员将全程指导本科生，并与杜克狐猴中心的教育和传播部门合作，为学生、公众和社交媒体观众创作新材料。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Gut Site and Gut Morphology Predict Microbiome Structure and Function in Ecologically Diverse Lemurs

• DOI: 10.1007/s00248-022-02034-4
• 发表时间: 2022-05
• 期刊: Microbial Ecology
• 影响因子: 3.6
• 作者: L. Greene;E. McKenney;William Gasper;Claudia Wrampelmeier;S. Hayer;E. Ehmke;J. B. Clayton

Forest access restores foraging and ranging behavior in captive sifakas

• DOI: 10.1002/zoo.21742
• 发表时间: 2022-10-17
• 期刊: ZOO BIOLOGY
• 影响因子: 1.3
• 作者: Greene，Lydia K.;Andriambeloson，Jean-Basile;Ehmke，Erin E.
• 通讯作者: Ehmke，Erin E.