NSF Postdoctoral Fellowship in Biology FY 2019: The Genetic Architecture of Hydraulic and Whole-plant Performance Under Cold Temperatures in Sunflower

2019 财年 NSF 生物学博士后奖学金：向日葵低温下水力和全株性能的遗传结构

• 批准号: 1907338
• 负责人: Jared Stewart
• 金额: $ 21.6万
• 依托单位: Stewart Jared J
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1907338&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; FY

This action funds an NSF National Plant Genome Initiative Postdoctoral Research Fellowship in Biology for FY 2019. The fellowship supports a research and training plan in a host laboratory for the Fellow who also presents a plan to broaden participation in biology. The title of the research and training plan for this fellowship to Dr. Jared J. Stewart is "The Genetic Architecture of Hydraulic and Whole-plant Performance Under Cold Temperature in Sunflower". The host institutions for the fellowship are USDA-ARS Water Management and Systems Research Unit (Fort Collins, CO), the University of Colorado-Boulder, and USDA-ARS Sunflower and Plant Biology Research Unit (Fargo, ND) and the sponsoring scientists are Drs. Sean M. Gleason, Nolan C. Kane, and Brent S. Hulke, respectively.Sunflower is one of the most widely produced oil crops across the globe. In the United States, many of the top sunflower-producing states experience episodic late-spring freezing temperatures. These freeze-thaw events pose a considerable threat to agricultural productivity through their dramatic impact on the water-transporting system of plants. This project aims to address this issue through identifying traits and genetics that confer superior performance under cold conditions in the common sunflower. Since this integrative project will occur at two USDA-ARS institutions with complementary missions as well as a public university, the results of this work should benefit ongoing efforts across multiple applied and fundamental disciplines. Furthermore, this experimental model could be adapted for other crops that are grown in locations with regularly occurring freeze-thaw events during the desired growing season. Training objectives for this project include characterizing hydraulic traits, identifying genetics underlying relevant traits, and developing high-throughput methods for efficient identification of superior crop lines. Broader impact activities during this project will include training and mentoring of undergraduate and graduate students as well as educational outreach at K-12 public schools and universities across northern Colorado.To meet agricultural demand in the face of climate change, plant functional traits and underlying genetics that allow superior plant performance under suboptimal environmental conditions must be identified. This project will contribute to this goal by characterizing the genetic architecture of hydraulic functioning in relation to high photosynthetic and whole-plant productivity under cold temperatures in sunflower. Relevant anatomical and functional traits will be characterized in plants grown under contrasting temperatures in climate-controlled growth chambers as well as plants grown in large-scale field trials. These results will be used to identify adaptive loci through both targeted and genome-wide association-mapping techniques. This project will feature over 300 accessions of the common sunflower, including the Sunflower Association Mapping (SAM) population, which encompasses 90% of the allelic diversity of cultivated sunflowers, as well as wild accessions that originate from a diversity of climates across its native range. Phenotypic and genotypic data from the wild accessions will be compared with climatic data from their respective sites of origin to provide ecological and evolutionary relevance, which could guide future breeding efforts for locations with exceptionally challenging climates. Phenotypic data from these investigations will be made available through the Sunflower Genome Database (https://www.sunflowergenome.org}, and genotypic data will be made available through the NCBI Short Read Archive database (https://www.ncbi.nlm.nih.gov/sra).Keywords: sunflower, hydraulic trait, abiotic stress, ecophysiology, association mapping, genomics, high throughput field-based phenotypingThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项行动为2019财年的NSF国家植物基因组计划生物学博士后研究奖学金提供资金。该研究金支持研究员在东道实验室的研究和培训计划，研究员还提出了扩大生物学参与的计划。Jared J. Stewart博士的研究和培训计划的标题是“低温下向日葵水力和全株性能的遗传结构”。 该奖学金的主办机构是美国农业部-农业研究所水管理和系统研究单位（柯林斯堡，CO），科罗拉多大学博尔德分校和美国农业部-农业研究所向日葵和植物生物学研究单位（法戈，ND），赞助科学家是Sean M.作者声明：C. Kane和布伦特S.向日葵是地球仪上最广泛生产的油料作物之一。在美国，许多顶级向日葵生产州都经历了春末的间歇性冰冻温度。这些冻融事件通过其对植物的水分运输系统的显著影响而对农业生产力构成相当大的威胁。该项目旨在通过确定普通向日葵在寒冷条件下具有上级性能的性状和遗传来解决这个问题。由于这一综合项目将在两个具有互补使命的USDA-ARS机构以及一所公立大学进行，因此这项工作的结果应有利于多个应用和基础学科的持续努力。此外，该实验模型可以适用于在期望的生长季节期间在具有定期发生的冻融事件的位置生长的其他作物。该项目的培训目标包括描述水力性状，识别相关性状的遗传学基础，以及开发高效识别上级作物品系的高通量方法。本项目期间影响更广的活动将包括对本科生和研究生的培训和指导，以及在北方科罗拉多的K-12公立学校和大学的教育推广。为了满足面对气候变化的农业需求，必须确定在次优环境条件下允许上级植物表现的植物功能性状和潜在遗传学。该项目将有助于这一目标的特点，在寒冷的温度下在向日葵高光合和全植物生产力的水力功能的遗传结构。相关的解剖和功能性状将在气候控制的生长室中的对比温度下生长的植物以及在大规模田间试验中生长的植物中进行表征。这些结果将用于通过靶向和全基因组关联作图技术来识别适应性位点。该项目将展示300多份普通向日葵的种质，包括向日葵关联作图（SAM）种群，其中包括90%的栽培向日葵等位基因多样性，以及来自其原产地气候多样性的野生种质。来自野生种质的表型和基因型数据将与来自其各自原产地的气候数据进行比较，以提供生态和进化相关性，这可以指导未来在气候异常具有挑战性的地区进行育种工作。这些调查的表型数据将通过向日葵基因组数据库（https：www.sunflowergenome.org}提供，基因型数据将通过NCBI Short Read Archive数据库（https：//www.ncbi.nlm.nih.gov/sra）提供。关键词：向日葵、水力性状、非生物胁迫、生态生理学、关联作图、基因组学、高通量基于田间的表型分析该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。

项目成果

Physiological trait networks enhance understanding of crop growth and water use in contrasting environments

• DOI: 10.1111/pce.14382
• 发表时间: 2022-07-07
• 期刊: PLANT CELL AND ENVIRONMENT
• 影响因子: 7.3
• 作者: Gleason, Sean M.;Barnard, Dave M.;Zhang, Huihui
• 通讯作者: Zhang, Huihui

Development and application of an inexpensive open-source dendrometer for detecting xylem water potential and radial stem growth at high spatial and temporal resolution

• DOI: 10.1093/aobpla/plae009
• 发表时间: 2024-03-20
• 期刊: AOB PLANTS
• 影响因子: 2.9
• 作者: Gleason，Sean M.;Stewart，Jared J.;Barnard，David M.
• 通讯作者: Barnard，David M.

Foliar sieve elements: Nexus of the leaf

• DOI: 10.1016/j.jplph.2021.153601
• 发表时间: 2021-12-23
• 期刊: JOURNAL OF PLANT PHYSIOLOGY
• 影响因子: 4.3
• 作者: Adams, William W., III;Stewart, Jared J.;Demmig-Adams, Barbara
• 通讯作者: Demmig-Adams, Barbara

Lemna as a Sustainable, Highly Nutritious Crop: Nutrient Production in Different Light Environments

浮萍作为一种可持续、高营养的作物：不同光照环境下的养分生产

• DOI: 10.3390/nutraceuticals2040027
• 发表时间: 2022
• 期刊: Nutraceuticals
• 作者: Polutchko, Stephanie K.;Stewart, Jared J.;McNamara, Maureen;Doherty Garcia, Naiara;López-Pozo, Marina;Adams, William W.;Demmig-Adams, Barbara
• 通讯作者: Demmig-Adams, Barbara

Photosynthesis and foliar vascular adjustments to growth light intensity in summer annual species with symplastic and apoplastic phloem loading

具有共质体和质外体韧皮部负荷的夏季一年生物种的光合作用和叶维管束对生长光强度的调节

• DOI: 10.1016/j.jplph.2021.153532
• 发表时间: 2021
• 期刊: Journal of Plant Physiology
• 影响因子: 4.3
• 作者: Polutchko, Stephanie K.;Stewart, Jared J.;Adams, William W.;Demmig-Adams, Barbara
• 通讯作者: Demmig-Adams, Barbara