Magnetic hybridmaterials in regenerative medicine: production, simulation, application and toxicologic investigations

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In this SPP the Section for Experimental Oncology and Nanomedicine (SEON) is mainly occupied with clinical relevant projects. Our work concentrates on the investigation of magnetic particles in a tissue-like environment or in a real matrix of the human body. Together with different partners within and outside the SPP we intend to work on the following projects:Generation of vessel transplants using magnetic hybrid materials.We are working on the generation of novel endothelializable vessel scaffolds. Those scaffolds will be endothelialized using magnetic fields directing magnetic particle-cell hybrids to the inner surface. Those artificial scaffolds should provide a tight and stable inner surface consisting of two different cell layers of endothelial and smooth muscle cells. In the beginning the scaffold will be composed of PTFE, PLGA or gelatin. The endothelialization will be performed using the magnetic cell seeding technique. The experience collected during the experiments will help to achieve a fast and efficient way for a long-time stable cell population of these new materials.Interactions of magnetic nanoparticles with fibrin-based networks - Investigation of thrombolysis.We are working on a new concept for the treatment of acute thrombosis using magnetic fields. This will provide a deep understanding of the interactions between particles and the elastic network of a thrombus and might provide the basis for future treatments of blood clots. The goal is to investigate whether it is possible to direct magnetic thrombolytica-binding hybrid materials into a thrombus using static or oscillating magnetic fields, thus dissolving the network from within. This would strongly reduce the risks of a systemic treatment with high doses of thrombolytica and extremely improve the prognosis of the treatment.Interaction of nanoparticles and magnetic hybrid materials with endothelial barriers.Important for the transport of drugs and the biodistribution of nanoparticles is, whether they are able to penetrate or injure endothelial barriers. Thus the toxicity of the materials and the potential to harm the endothelial barriers must be investigated. Therefore we will perform experiments using tightly grown epithelial cells as well as multilayered co-cultures of smooth muscle cells and epithelial cells. Additionally we will perform experiments using brain-barrier and placenta models. We will investigate how particles penetrate biological barriers and whether the stabilizing corona changes during migration.