Magnetic hybridmaterials in regenerative medicine: production, simulation, application and toxicologic investigations

再生医学中的磁性杂化材料：生产、模拟、应用和毒理学研究

• 批准号: 237991100
• 负责人: Professor Dr. Christoph Alexiou
• 金额: --
• 依托单位: Hals-Nasen-Ohren-Klinik, Kopf- und Halschirurgie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/237991100?language=en
• 关键词: Magnetic; hybridmaterials; regenerative; medicine; production

In this SPP the Section for Experimental Oncology and Nanomedicine (SEON) is mainly occupied with clinical relevant projects. Our work concentrates on the investigation of magnetic particles in a tissue-like environment or in a real matrix of the human body. Together with different partners within and outside the SPP we intend to work on the following projects:Generation of vessel transplants using magnetic hybrid materials.We are working on the generation of novel endothelializable vessel scaffolds. Those scaffolds will be endothelialized using magnetic fields directing magnetic particle-cell hybrids to the inner surface. Those artificial scaffolds should provide a tight and stable inner surface consisting of two different cell layers of endothelial and smooth muscle cells. In the beginning the scaffold will be composed of PTFE, PLGA or gelatin. The endothelialization will be performed using the magnetic cell seeding technique. The experience collected during the experiments will help to achieve a fast and efficient way for a long-time stable cell population of these new materials.Interactions of magnetic nanoparticles with fibrin-based networks - Investigation of thrombolysis.We are working on a new concept for the treatment of acute thrombosis using magnetic fields. This will provide a deep understanding of the interactions between particles and the elastic network of a thrombus and might provide the basis for future treatments of blood clots. The goal is to investigate whether it is possible to direct magnetic thrombolytica-binding hybrid materials into a thrombus using static or oscillating magnetic fields, thus dissolving the network from within. This would strongly reduce the risks of a systemic treatment with high doses of thrombolytica and extremely improve the prognosis of the treatment.Interaction of nanoparticles and magnetic hybrid materials with endothelial barriers.Important for the transport of drugs and the biodistribution of nanoparticles is, whether they are able to penetrate or injure endothelial barriers. Thus the toxicity of the materials and the potential to harm the endothelial barriers must be investigated. Therefore we will perform experiments using tightly grown epithelial cells as well as multilayered co-cultures of smooth muscle cells and epithelial cells. Additionally we will perform experiments using brain-barrier and placenta models. We will investigate how particles penetrate biological barriers and whether the stabilizing corona changes during migration.

在该SPP中，实验肿瘤学和纳米医学（SEON）部门主要从事临床相关项目。我们的工作集中在研究磁性粒子在类组织环境或人体的真实的基质中的作用。我们与SPP内外的不同合作伙伴一起，计划开展以下项目：使用磁性混合材料生成血管移植物我们正在研究新型可内皮化血管支架的生成。这些支架将使用磁场引导磁性颗粒-细胞混合物到内表面来内皮化。这些人工支架应该提供由内皮细胞和平滑肌细胞的两个不同细胞层组成的紧密且稳定的内表面。开始时，支架将由PTFE、PLGA或明胶组成。将使用磁性细胞接种技术进行内皮化。在实验过程中收集的经验将有助于实现这些新材料的长期稳定的细胞群的快速和有效的方法。磁性纳米颗粒与纤维蛋白网络的相互作用-溶栓研究我们正在研究一种使用磁场治疗急性血栓形成的新概念。这将提供对血栓颗粒和弹性网络之间相互作用的深入理解，并可能为未来治疗血栓提供基础。目的是研究是否有可能使用静态或振荡磁场将磁性溶栓剂结合混合材料导入血栓，从而从内部溶解网络。这将大大降低高剂量溶血栓剂的全身治疗的风险，并极大地改善治疗的预后。纳米颗粒和磁性杂化材料与内皮屏障的相互作用对于药物的转运和纳米颗粒的生物分布至关重要，它们是否能够穿透或损伤内皮屏障。因此，必须研究材料的毒性和损害内皮屏障的可能性。因此，我们将使用紧密生长的上皮细胞以及平滑肌细胞和上皮细胞的多层共培养物进行实验。此外，我们将使用脑屏障和胎盘模型进行实验。我们将研究粒子如何穿透生物屏障，以及在迁移过程中稳定电晕是否发生变化。