Analysis of the activation of chloroplast translation and RNA stabilization by PPR proteins and PPR protein derived sRNAs

PPR 蛋白和 PPR 蛋白衍生的 sRNA 对叶绿体翻译和 RNA 稳定的激活作用的分析

• 批准号: 238779258
• 负责人: Professor Dr. Christian Schmitz-Linneweber
• 金额: --
• 依托单位: Arbeitsgruppe Molekulare Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/238779258?language=en
• 关键词: Analysis; activation; chloroplast; translation; RNA

PPR proteins are essential for all steps in chloroplast RNA metabolism and thus for chloroplast biogenesis. Each PPR protein binds with high selectivity to just a few plastid RNAs. A subset of PPR proteins impacts the stability and translation of mRNAs. These PPR proteins cause footprints that accumulate as short RNAs (sRNAs). This proposal has two main goals: 1. Understanding the mechanistic details of how PPR proteins activate translation and how they stabilize mRNAs. 2. Understanding the role of sRNAs as potential regulators of chloroplast gene expression.

PPR蛋白对于叶绿体RNA代谢的所有步骤以及因此对于叶绿体生物发生是必需的。每一个PPR蛋白质仅与少数质体RNA高选择性结合。PPR蛋白的一个子集影响mRNA的稳定性和翻译。这些PPR蛋白导致足迹积累为短RNA（sRNA）。该提案有两个主要目标：1。了解PPR蛋白如何激活翻译以及如何稳定mRNA的机制细节。2.了解sRNAs作为叶绿体基因表达的潜在调节因子的作用。

项目成果

A Nucleus-Encoded Chloroplast Phosphoprotein Governs Expression of the Photosystem I Subunit PsaC in Chlamydomonas reinhardtii

• DOI: 10.1105/tpc.15.00725
• 发表时间: 2016-05-01
• 期刊: PLANT CELL
• 影响因子: 11.6
• 作者: Douchi, Damien;Qu, Yujiao;Goldschmidt-Clermont, Michel
• 通讯作者: Goldschmidt-Clermont, Michel