RUI: Alternative splicing in the regulation of plant innate immunity

RUI：选择性剪接在植物先天免疫调节中的作用

• 批准号: 1923069
• 负责人: Xiao-Ning Zhang
• 金额: $ 65万
• 依托单位: Saint Bonaventure University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1923069&HistoricalAwards=false
• 关键词: RUI; Alternative; splicing; regulation; plant

Plant diseases threaten crop yield and lead to billions of dollars of losses each year worldwide. RNA alternative splicing is a conserved mechanism to increase the diversity of transcripts and protein functions, and has been implicated in plant defense against pathogens. This project will elucidate the molecular basis governing the interplay between RNA alternative splicing and plant defense. This will contribute to the current understanding of plant defense mechanisms and eventually facilitate developing strategies to improve crop plants with better balanced growth and defense, and ultimately with higher productivity. This project will be used as a platform to engage undergraduate students, K-12 teachers and high school students from rural WNY, including the Native American population, in a series of STEM career readiness training exercises in the summer that can be replicated at other undergraduate institutions. Some research components of this project will be integrated in classroom teaching and laboratory investigations. Throughout the courses, students will practice critical thinking, proposal writing and professional presentations. Exercises will be provided to enhance resource and data management skills in students. This project aims to provide students a first-hand experience and to increase their competencies for future careers in the STEM fields. Teaching materials developed in this project will be made available for others to adapt and/or adopt.Strong evidence from the preliminary work indicates that a splicing regulator, SR45, acts as a suppressor of innate immunity in Arabidopsis. This project aims to elucidate how SR45, regulates plant innate immunity, a novel role of the gene that has never been characterized before. To achieve this goal, the following objectives are proposed: (1) to investigate how SR45 affects plant innate immunity in response to a variety of pathogen species using genetics approach and a suite of defense assays; (2) to confirm and characterize the involvement of putative alternatively spliced SR45-associated transcripts in plant immune response using molecular and genetic approaches; (3) to identify new pathogen-induced and SR45-dependent alternative splicing events in plant immune responses using RNA-seq with pathogen-infected inducible SR45 silencing line leaf samples and mock-treated leaf and the non-silencing line as control.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物病害威胁作物产量，每年在全世界造成数十亿美元的损失。RNA选择性剪接是一种保守的增加转录本和蛋白质功能多样性的机制，并与植物对病原体的防御有关。本项目将阐明RNA选择性剪接和植物防御之间相互作用的分子基础。这将有助于目前对植物防御机制的理解，并最终促进发展战略，以改善作物植物更好地平衡生长和防御，并最终提高生产力。该项目将被用作一个平台，吸引来自WNY农村的本科生，K-12教师和高中生，包括美洲原住民，参加夏季的一系列STEM职业准备培训活动，这些活动可以在其他本科院校复制。该项目的一些研究内容将整合到课堂教学和实验室调查中。在整个课程中，学生将练习批判性思维，提案写作和专业演讲。将提供练习，以提高学生的资源和数据管理技能。该项目旨在为学生提供第一手经验，并提高他们在STEM领域未来职业生涯的能力。本项目开发的教学材料将提供给其他人改编和/或采用。前期工作的有力证据表明，剪接调节因子SR 45在拟南芥中起着先天免疫抑制剂的作用。该项目旨在阐明SR 45如何调节植物先天免疫，这是该基因以前从未被描述过的新作用。为了实现这一目标，本论文提出了以下目标：（1）利用遗传学方法和一系列防御试验研究SR 45对植物天然免疫应答的影响;（2）利用分子和遗传学方法确定和表征SR 45相关转录本在植物免疫应答中的作用;（3）使用RNA-seq鉴定植物免疫应答中新的病原体诱导的和SR 45依赖性可变剪接事件，其中病原体感染的诱导型SR 45沉默系叶样品和模拟处理的叶和非诱导型SR 45沉默系叶样品的选择性剪接事件。该奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的知识价值和更广泛的影响审查标准。

项目成果

The flowering time regulator FLK controls pathogen defense in Arabidopsis thaliana

• DOI: 10.1093/plphys/kiad021
• 发表时间: 2023-02-09
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Fabian，Matthew;Gao，Min;Lu，Hua
• 通讯作者: Lu，Hua