Collaborative Research: RUI: Combined spatial and temporal analyses of population connectivity during a northern range expansion

合作研究：RUI：北部范围扩张期间人口连通性的时空综合分析

• 批准号: 1924537
• 负责人: Crow White
• 金额: $ 41.56万
• 依托单位: California Polytechnic State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1924537&HistoricalAwards=false
• 关键词: Collaborative; Research; RUI; Combined; spatial

Where do young marine fish and shellfish come from? This project aims to improve our understanding of how coastal marine populations are connected in space and time. Coastal populations are replenished through the arrival of minuscule larvae that have been dispersed for weeks to months in the open ocean after spawning at remote sites. The combination of the long dispersal period of marine fish and shellfish larvae and the varying ocean currents results in complex patterns of "connectivity" among populations near and far. Identifying these patterns of connectivity is fundamental to marine science and critical for effective fisheries management and conservation, yet it remains an unresolved component of marine ecology. The study species is currently expanding its biogeographic range up the U.S. west coast. By genetically analyzing individuals from across the species' range, including offspring spawned in the laboratory by experimentally-crossed individuals collected in the field from throughout the species historical and expanded range, certain genes can serve to differentiate populations along the coast. The team leverages the statistical power of these geographically-informative genes to assign thousands of young collected in the field to the source populations that spawned them (across the species' range and over multiple years). The team then quantifies patterns of connectivity over multiple years, and tests fundamental hypotheses on the spatial scale, temporal variability, biogeographic patterns, and biophysical drivers of population connectivity. The project trains approximately two dozen U.S. university students in molecular ecology and marine science, as well as creating intellectual linkages among Ph.D.-granting and non-Ph.D.-granting universities. The project also supports further development of a K-12 education program that uses SCUBA diving and videography to teach elementary school students Next Generation Science Standards and train them for careers in science, technology, engineering and mathematics. Using a kelp forest gastropod and fisheries species (Kellet's whelk, Kelletia kelletii), this project combines genome-wide Restriction site Associated DNA (RAD) loci with transcriptomic loci identified from common-garden laboratory crosses of individuals from the species' historical and expanded range to identify geographically-informative loci that maximize power for individual assignment testing. Leveraging the combined power of these loci, genetic assignment of approximately three thousand recruit samples to 20 putative source populations allows the team to construct three independent years of connectivity matrices and test some of the most fundamental questions in marine ecology, including: 1) Are marine populations open or closed and at what scales? 2) To what degree is the evolutionary pattern of gene flow represented by single versus multiple generations of connectivity events? And, 3) How spatially heterogeneous and temporally variable is population connectivity? Can one year of connectivity data predict anything about the next? Additionally, by focusing on a range-expanding species with common life history traits, the team addresses a number of questions with broad applicability and significant ecological and societal implications: 4) How much is population connectivity influenced by post-recruitment demographic and evolutionary processes? 5) How well-connected are historic- and expanded-range populations? And, of particular relevance to climate change, 6) Are El Nino oceanographic conditions, which are predicted to increase in frequency and intensity this century, driving the poleward range expansion of this coastal marine species? By coupling common-garden experimental crosses to identify maximally-informative transcriptomic loci with genomic RAD analysis of field samples, this project aims to accurately and precisely quantify marine population connectivity in high gene flow species with large population sizes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

年轻的海洋鱼类和贝类来自哪里？该项目旨在提高我们对沿海海洋种群在空间和时间上如何联系的理解。沿海种群的补充是通过到达微小的幼虫，这些幼虫在偏远的地方产卵后分散在公海上数周至数月。海洋鱼类和贝类幼虫的长期扩散期与不断变化的洋流相结合，导致远近种群之间的复杂“联系”模式。确定这些连接模式对海洋科学至关重要，对有效的渔业管理和养护至关重要，但它仍然是海洋生态中一个尚未解决的问题。该物种目前正在美国西海岸扩大其地理范围。通过对来自物种分布区的个体进行遗传分析，包括在实验室中由从整个物种历史分布区和扩展分布区收集的实验性杂交个体繁殖的后代，某些基因可以用于区分沿着的种群。该团队利用这些地理信息基因的统计能力，将野外收集的数千只幼仔分配给产生它们的源种群（跨越物种的范围和多年）。然后，该团队量化了多年来的连通性模式，并测试了关于空间尺度、时间变异性、地理模式和人口连通性的生物物理驱动因素的基本假设。该项目培训了大约20多名美国大学生从事分子生态学和海洋科学方面的工作，并在博士生之间建立了智力联系。授予和非博士学位-授予大学。该项目还支持进一步发展K-12教育计划，该计划使用水肺潜水和录像技术向小学生教授下一代科学标准，并培训他们从事科学，技术，工程和数学方面的职业。使用海带森林腹足类和渔业物种（凯氏海螺，Kelletia kelletii），该项目结合了全基因组限制性内切酶位点相关DNA（RAD）基因座与转录组基因座从共同花园实验室杂交的物种的历史和扩大范围的个人，以确定地理信息的基因座，最大限度地提高个人分配测试的权力。利用这些基因座的综合力量，将大约3000个新兵样本遗传分配给20个假定的来源种群，使该团队能够构建三个独立年份的连接矩阵，并测试海洋生态学中一些最基本的问题，包括：1）海洋种群是开放的还是封闭的，规模是多少？2)单代与多代连接事件在多大程度上代表了基因流的进化模式？以及，3）如何空间异质性和时间变量是人口连接？一年的连接数据能预测下一年的情况吗？此外，通过关注具有共同生活史特征的范围扩大物种，该团队解决了一些具有广泛适用性和重大生态和社会影响的问题：4）人口连接性受招募后人口统计和进化过程的影响有多大？5)历史种群和扩展范围种群之间的联系有多紧密？而且，与气候变化特别相关的是，6）预计本世纪频率和强度将增加的厄尔尼诺海洋条件是否推动了这种沿海海洋物种向极地的范围扩张？该项目通过将普通花园实验杂交与野外样本的基因组RAD分析相结合，以确定信息量最大的转录组基因座，旨在准确和精确地量化具有大种群规模的高基因流物种的海洋种群连接性。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

De novo genome and transcriptome assembly of Kelletia kelletii, a coastal gastropod and fisheries species exhibiting a northern range expansion

• DOI: 10.3389/fmars.2023.1278131
• 发表时间: 2023-12-05
• 期刊: FRONTIERS IN MARINE SCIENCE
• 影响因子: 3.7
• 作者: Daniels，Benjamin N.;Andrasz，Cassidy L.;Davidson，Jean M.
• 通讯作者: Davidson，Jean M.

Rapid genetic adaptation to a novel ecosystem despite a large founder event

尽管发生了大型创始人事件，但基因仍能快速适应新的生态系统

• DOI: 10.1111/mec.17121
• 发表时间: 2023
• 期刊: Molecular Ecology
• 影响因子: 4.9
• 作者: Sparks, Morgan M.;Schraidt, Claire E.;Yin, Xiaoshen;Seeb, Lisa W.;Christie, Mark R.
• 通讯作者: Christie, Mark R.

Length-Weight Relationship of the Kelp Forest Gastropod and Emerging Fisheries Species, Kellet's Whelk, Kelletia kelletii

海带林腹足动物与新兴渔业物种海螺、海螺的长度-重量关系

• DOI: 10.2983/035.042.0313
• 发表时间: 2023
• 期刊: Journal of Shellfish Research
• 影响因子: 1.3
• 作者: Gosnell, J. Stephen;Clare, Xochitl S.;Whited, Dillon;Chiu, Jennifer;Huie, Stephen;Zacherl, Danielle;White, Crow
• 通讯作者: White, Crow