BEE: Spatio-temporal factors shaping symbiotic networks: a case study with cyanolichens

BEE：塑造共生网络的时空因素：氰基地衣的案例研究

• 批准号: 1929994
• 负责人: Francois Lutzoni
• 金额: $ 41.31万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1929994&HistoricalAwards=false
• 关键词: BEE; Spatio; temporal; factors; shaping

Symbiotic interactions, including parasitism, commensalism and mutualism, form complex networks that are common in nature. Ultimately, a more complete understanding of life can be accomplished only through the study of all symbiotically interacting organisms. This study aims to understand the processes that shape those networks using cyanolichens, which are iconic symbioses consisting mainly of mutually beneficial fungi and cyanobacteria. Questions that will be addressed by this project include: Does the abundance of various free-living cyanobacteria in nature dictate the main lichen partner pairs (one fungus, one cyanobacterium) who shape lichen communities? Do broadly distributed cyanobacteria who associate with many fungal species (generalists) do so mostly with closely related fungal species? What are the biomolecules that determine which cyanobacteria interact with which fungal partners? How do the rules of association for lichen partners differ when compared to other symbiotic systems? This project includes training and educational components for graduate and undergraduate students, the dissemination of new methods for innovative research on symbiotic systems, outreach activities, and the development of a new advising model to help underrepresented students interested in science and engineering reach their academic goals. The main focus of this study is to determine how mutualistic networks are shaped by evolution and environmental gradients at different spatial scales. To reach this goal, a fully integrated approach combining evolutionary biology and ecology will be implemented. Recent studies of the lichen model genus Peltigera and its Nostoc symbionts have revealed: (i) that Peltigera species are mostly specialized on generalist Nostoc phylogroups, (ii) that the interactions and the high level of specialization by the fungus are phylogenetically conserved, and (iii) that bioclimatic factors, at an intra-biome scale, are more limiting than cyanobiont availability for explaining geographic distributions of Peltigera species. This study will capitalize on the large-scale, systematic, and multi-taxon, monitoring program of the Alberta Biodiversity Monitoring Institute (ABMI). This project represents a unique opportunity to test three important drivers of symbiotic network assembly: (1) Spatial scales and gradients - Are interactions well predicted by environmental biotic and abiotic properties, and/or spatial overlap due to stochastic dispersal? (2) Phylogenetic history - Do Nostoc symbionts show phylogenetic conservatism in their selection of Peltigera partners, as shown by Peltigera towards their Nostoc partners? (3) Specific recognition molecules - Are Nostoc genes differentially expressed in lichen thalli vs a free-living state, and do they explain the observed specificity between symbiotic partners in nature?This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

共生相互作用，包括寄生、共生和互惠，形成自然界中常见的复杂网络。最终，只有通过研究所有共生相互作用的生物体才能实现对生命的更全面的了解。这项研究的目的是了解使用蓝藻形成这些网络的过程，蓝藻是主要由互利真菌和蓝藻组成的标志性共生体。该项目将解决的问题包括：自然界中各种自由生活的蓝细菌的丰度是否决定了塑造地衣群落的主要地衣伴侣对（一种真菌，一种蓝细菌）？广泛分布的蓝细菌与许多真菌物种（通才），这样做大多与密切相关的真菌物种？决定哪些蓝藻与哪些真菌伴侣相互作用的生物分子是什么？与其他共生系统相比，地衣伴侣的联合规则有何不同？该项目包括对研究生和本科生的培训和教育部分、传播共生系统创新研究的新方法、外展活动以及开发新的咨询模式，以帮助对科学和工程感兴趣的代表性不足的学生实现他们的学术目标。 本研究的主要重点是确定如何互利网络的形状在不同的空间尺度上的进化和环境梯度。为了实现这一目标，将实施一种结合进化生物学和生态学的完全集成的方法。最近的地衣模式属Peltigera及其念珠藻共生体的研究表明：（i）Peltigera物种大多是专门的通才念珠藻群，（ii）真菌的相互作用和高水平的专业化是遗传保守的，和（iii）生物气候因素，在生物群落内的规模，更限制比cyanobiont可用性解释Peltigera物种的地理分布。这项研究将利用大规模的，系统的，多分类，监测计划的阿尔伯塔生物多样性监测研究所（ABMI）。这个项目代表了一个独特的机会来测试共生网络组装的三个重要驱动因素：（1）空间尺度和梯度-相互作用是否可以通过环境生物和非生物特性和/或由于随机扩散造成的空间重叠来预测？(2)系统发育史-念珠藻共生体在选择Peltigera伴侣时是否表现出系统发育保守性，正如Peltigera对念珠藻伴侣所表现的那样？(3)特异性识别分子-念珠藻基因在地衣菌体和自由生活状态下的差异表达，它们是否解释了自然界中共生伙伴之间观察到的特异性？该奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估的支持。

项目成果

Ancient Rapid Radiation Explains Most Conflicts Among Gene Trees and Well-Supported Phylogenomic Trees of Nostocalean Cyanobacteria

古代快速辐射解释了念珠藻蓝藻基因树和良好支持的系统发育树之间的大多数冲突

• DOI: 10.1093/sysbio/syad008
• 发表时间: 2023
• 期刊: Systematic Biology
• 影响因子: 6.5
• 作者: Pardo-De la Hoz, Carlos J.;Magain, Nicolas;Piatkowski, Bryan;Cornet, Luc;Dal Forno, Manuela;Carbone, Ignazio;Miadlikowska, Jolanta;Lutzoni, François
• 通讯作者: Lutzoni, François

High species richness in the lichen genus Peltigera (Ascomycota, Lecanoromycetes): 34 species in the dolichorhizoid and scabrosoid clades of section Polydactylon, including 24 new to science

Peltigera 地衣属（子囊菌门、Lecanoromycetes）物种丰富度高：多指菌科的长根菌和粗根菌分支中有 34 种，其中包括 24 种新科学物种

• DOI: 10.3767/persoonia.2023.51.01
• 发表时间: 2023
• 期刊: Persoonia - Molecular Phylogeny and Evolution of Fungi
• 作者: Magain, N.;Miadlikowska, J.;Goffinet, B.;Goward, T.;Pardo-De la Hoz, C.J.;Jüriado, I.;Simon, A.;Mercado-Díaz, J.A.;Barlow, T.;Moncada, B.
• 通讯作者: Moncada, B.

Towards a nomenclatural clarification of the Peltigera ponojensis/monticola clade including metagenomic sequencing of type material and the introduction of P. globulata Miadl. & Magain sp. nov.

• DOI: 10.1017/s0024282923000373
• 发表时间: 2023-09-01
• 期刊: LICHENOLOGIST
• 影响因子: 1.4
• 作者: Miadlikowska，Jolanta;Magain，Nicolas;Lutzoni，Francois
• 通讯作者: Lutzoni，Francois