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The significance of photosystem II supercomplexes in light acclimation of Arabidopsis thaliana

光系统II超复合体在拟南芥光驯化中的意义

基本信息

批准号：
239465926
负责人：
Dr. Lars Dietzel
金额：
--
依托单位：
Lehrstuhl für Biochemie der Pflanzen
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/239465926?language=en
关键词：
significance photosystem II supercomplexes light

项目摘要

Photosynthesis is the major energy fixing process on earth. Higher plants as the model plant Arabidopsis thaliana regulate their photosynthetic capacity very fast and efficiently in order to allow for high photosynthetic performance and at the same time protection from oxidative damage. Photosystem (PS) II supercomplexes are of substantial importance for regulation of photosynthesis. Although the structure of PSII supercomplexes has been determined, the process of PSII supercomplex assembly and PSII supercomplex remodeling during light acclimation is poorly understood. We revealed in own previous work that the PSII associated protein Psb27 is required for assembly and/or stability of PSII supercomplexes while PSII supercomplex remodeling is induced by PSII core protein phosphorylation. Interestingly, the major phosphorylation site of the PSII subunit CP43 might be a putative Psb27 binding site. Within this project we aim to elucidate the assembly and regulation of PSII supercomplexes. To this end i) we will utilize immuno-biochemical approaches to determine the localization and function of the PSII associated factor Psb27. Additionally, we will use in planta tagged Psb27 lines in order to identify interacting proteins. ii) We will investigate the kinetics of PSII core phosphorylation in relation to the altered functionality of PSII supercomplexes. With fluorometrical and spectroscopical methods we will assess concomitant changes in light harvesting, light energy distribution and transfer. To this end we will use mutant lines with altered thylakoid complex phosphorylation in combination with defined light regimes. By this means we will be able to assign physiological functions of differentially phosphorylated PSII supercomplexes. iii) We will investigate the physiological impact of PSII remodeling on the ability to acclimate to different light environments using Arabidopsis lines impaired in assembly or regulation of PSII supercomplexes. These mutants will be subjected to fluctuating light conditions and tested for their photosynthetic performance. The knowledge obtained about PSII supercomplexes and their remodeling will enable us to evaluate the effects of the light environment on plant growth and yield. In the future the productivity of agricultural important plants might benefit from this knowledge.

光合作用是地球上主要的能量固定过程。高等植物作为模式植物拟南芥（Arabidopsis thaliana）非常快速有效地调节其光合能力，以允许高光合性能，同时保护免受氧化损伤。光系统II超复合物对光合作用的调控具有重要意义。虽然PSII超复合物的结构已被确定，PSII超复合物组装和PSII超复合物在光适应过程中的重塑过程知之甚少。我们揭示了在自己以前的工作中，PSII相关蛋白Psb 27所需的组装和/或PSII超复合物的稳定性，而PSII超复合物的重塑是由PSII核心蛋白磷酸化诱导。有趣的是，PSII亚基CP 43的主要磷酸化位点可能是推定的Psb 27结合位点。在这个项目中，我们的目标是阐明PSII超复合物的组装和调节。为此，i）我们将利用免疫生化方法来确定PSII相关因子Psb 27的定位和功能。此外，我们将在植物中使用标记的Psb 27系，以确定相互作用的蛋白质。ii）我们将研究PSII核心磷酸化的动力学与PSII超复合物功能改变的关系。与荧光和光谱的方法，我们将评估伴随的变化，光收集，光能分布和转移。为此，我们将使用具有改变的类囊体复合物磷酸化的突变株系与确定的光制度组合。通过这种方法，我们将能够分配差异磷酸化PSII超复合物的生理功能。iii）我们将研究PSII重构对适应不同光环境的能力的生理影响，使用在PSII超复合物的组装或调节中受损的拟南芥品系。这些突变体将经受波动的光照条件并测试其光合性能。获得有关PSII超复合物及其重塑的知识将使我们能够评估光环境对植物生长和产量的影响。在未来，农业重要植物的生产力可能会受益于这一知识。