Periplasmic Chaperone Network Organization and Mechanism
周质伴侣网络组织和机制
基本信息
- 批准号:1931211
- 负责人:
- 金额:$ 120万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-07-01 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This discovery project investigates the maturation processes of beneficial microorganisms found in humans. These microbes have sugar- and fat-filled membrane surfaces that encapsulate their cells. Proteins are embedded within the sugar/fat mixture, and their proper incorporation is essential for the integrity and functions of these surfaces. This project addresses how these three types of molecules come together. A series of systematic investigations will be conducted to determine the molecular structures of helper proteins that play key roles in the assembly process. A second part of the project will use pictures of living cells to discover the surface distribution of these helper proteins throughout the cell. Are these helper proteins clumping all together in a cell? Or are they sprinkled evenly throughout? In addition, the PI will broaden the participation in science through research opportunities for graduate and undergraduate students, by nurturing a more inclusive climate in graduate and undergraduate STEM education, and by teaching using evidence-based best practices. The goals of this project are to investigate the periplasmic chaperone network organization and mechanism. This will be achieved using two general approaches. The first is to determine the solution structures of two of the soluble chaperone proteins in complex with an unfolded outer membrane protein. This will be accomplished by integrating information from orthogonal, biophysical methods and computational modeling. The outcome from this effort will be novel structures that provide key information on how these chaperones ensure efficient outer membrane protein biogenesis by maintaining unfolded membrane proteins in solution. The second fundamental effort addressed in this project is to examine the periplasmic distribution of a key chaperone in living cells using imaging techniques. The main question in this latter effort is to evaluate whether or not soluble chaperones are well mixed in this unique cellular compartment that lacks any external energy source. Together the outcomes will provide a fundamental understanding of membrane protein biogenesis in bacteria. This information can be used to infer mechanistic insights into homologous system in mitochondria of eukaryotes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
这个发现项目研究了在人类中发现的有益微生物的成熟过程。这些微生物有糖和脂肪填充的膜表面,包裹着它们的细胞。蛋白质嵌入糖/脂肪混合物中,它们的适当掺入对于这些表面的完整性和功能至关重要。该项目解决了这三种类型的分子如何结合在一起。将进行一系列系统的研究,以确定在组装过程中发挥关键作用的辅助蛋白的分子结构。该项目的第二部分将使用活细胞的照片来发现这些辅助蛋白在整个细胞中的表面分布。这些辅助蛋白在细胞中聚集在一起吗?还是说,他们都是被人欺负的呢?此外,PI将通过为研究生和本科生提供研究机会、在研究生和本科STEM教育中营造更具包容性的氛围以及使用基于证据的最佳实践进行教学,扩大对科学的参与。本项目的目的是研究周质分子伴侣网络的组织和机制。这将使用两种一般方法来实现。第一个是确定两个可溶性伴侣蛋白与未折叠的外膜蛋白复合的溶液结构。这将通过整合来自正交、生物物理方法和计算建模的信息来实现。这项工作的结果将是新的结构,提供了关键信息,这些分子伴侣如何确保有效的外膜蛋白的生物合成,保持未折叠的膜蛋白在溶液中。在这个项目中解决的第二个基本的努力是使用成像技术检查活细胞中的一个关键分子伴侣的周质分布。在后一种努力中的主要问题是评估可溶性伴侣蛋白是否在缺乏任何外部能量来源的这种独特的细胞隔室中良好混合。这些结果将共同提供对细菌膜蛋白生物合成的基本理解。该奖项反映了NSF的法定使命,通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Domain interactions determine the conformational ensemble of the periplasmic chaperone SurA
- DOI:10.1002/pro.3924
- 发表时间:2020-08
- 期刊:
- 影响因子:8
- 作者:Dagan C. Marx;Mathis J. Leblanc;A. Plummer;S. Krueger;K. Fleming
- 通讯作者:Dagan C. Marx;Mathis J. Leblanc;A. Plummer;S. Krueger;K. Fleming
Generation of unfolded outer membrane protein ensembles defined by hydrodynamic properties
由流体动力学特性定义的未折叠外膜蛋白整体的生成
- DOI:10.1007/s00249-023-01639-y
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Devlin, Taylor;Fleming, Patrick J.;Loza, Nicole;Fleming, Karen G.
- 通讯作者:Fleming, Karen G.
Revisiting macromolecular hydration with HullRadSAS.
使用 HullRadSAS 重新审视大分子水合。
- DOI:10.1007/s00249-022-01627-8
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Fleming,PatrickJ;Correia,JohnJ;Fleming,KarenG
- 通讯作者:Fleming,KarenG
SurA is a cryptically grooved chaperone that expands unfolded outer membrane proteins
- DOI:10.1073/pnas.2008175117
- 发表时间:2020-10
- 期刊:
- 影响因子:0
- 作者:Dagan C. Marx;A. Plummer;Anneliese M. Faustino;Taylor A. Devlin;Michaela A. Roskopf;Mathis J. Leblanc;Henry J. Lessen;B. T. Amann;P. Fleming;S. Krueger;S. Fried;K. Fleming
- 通讯作者:Dagan C. Marx;A. Plummer;Anneliese M. Faustino;Taylor A. Devlin;Michaela A. Roskopf;Mathis J. Leblanc;Henry J. Lessen;B. T. Amann;P. Fleming;S. Krueger;S. Fried;K. Fleming
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Karen Fleming其他文献
Exclusive Breastfeeding Rates Upon Hospital Discharge at a Tertiary Centre Prior to and During the COVID-19 Pandemic
- DOI:
10.1016/j.jogc.2024.102669 - 发表时间:
2024-12-01 - 期刊:
- 影响因子:
- 作者:
Hannah Matthews;Jo Watson;Sue Hermann;Karen Fleming - 通讯作者:
Karen Fleming
Grossesse: Une fenêtre sur la santé cardiovasculaire future de la femme
- DOI:
- 发表时间:
2013-10 - 期刊:
- 影响因子:3.1
- 作者:
Karen Fleming - 通讯作者:
Karen Fleming
A Qualitative Exploration of How the Pregnancy and Postpartum Journey was Shaped by the COVID-19 Pandemic
- DOI:
10.1016/j.jogc.2024.102489 - 发表时间:
2024-05-01 - 期刊:
- 影响因子:
- 作者:
Karen Fleming - 通讯作者:
Karen Fleming
30 - Avoiding Diabetes after Pregnancy Trial in Moms (Adapt-M): Feasibility of a Health-Coaching Program for Women with Recent Gestational Diabetes Mellitus
- DOI:
10.1016/j.jcjd.2018.08.033 - 发表时间:
2018-10-01 - 期刊:
- 影响因子:
- 作者:
Faith Delos-Reyes;Andrea J. Glenn;Howard Berger;Stephanie De Sequeira;Denice Feig;Karen Fleming;Shannan Grant;Ilana Halperin;Paula Harvey;Xinyun Liang;Geetha Mukerji;Jennifer Price;Joel Ray;Ravi Retnakaran;Diana Sherifali;Kevin Thorpe;Lorraine Lipscombe - 通讯作者:
Lorraine Lipscombe
Offspring Birthweight and Long-Term Risk of Maternal Diabetes
- DOI:
10.1016/j.jcjd.2021.09.028 - 发表时间:
2021-11-01 - 期刊:
- 影响因子:
- 作者:
Tina Nham;Stephanie Read;Howard Berger;Denice Feig;Karen Fleming;Joel Ray;Laura Rosella;Baiju Shah;Lorraine Lipscombe - 通讯作者:
Lorraine Lipscombe
Karen Fleming的其他文献
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{{ truncateString('Karen Fleming', 18)}}的其他基金
Towards a Holistic Model for Outer Membrane Protein Sorting in the Periplasm
周质外膜蛋白质分选的整体模型
- 批准号:
1412108 - 财政年份:2014
- 资助金额:
$ 120万 - 项目类别:
Continuing Grant
Conference: 2010 Biomolecular Interactions & Methods GRC and GRS to be held in Galveston, Texas on January 16-22, 2010
会议:2010 生物分子相互作用
- 批准号:
0949012 - 财政年份:2010
- 资助金额:
$ 120万 - 项目类别:
Standard Grant
Biophysical Analysis of Chaperone Influences on Membrane Protein Folding
伴侣分子对膜蛋白折叠影响的生物物理分析
- 批准号:
0919868 - 财政年份:2009
- 资助金额:
$ 120万 - 项目类别:
Continuing Grant
Thermodynamic Analysis of Transmembrane Beta Barrel Self-Association
跨膜 Beta 桶自缔合的热力学分析
- 批准号:
0423807 - 财政年份:2004
- 资助金额:
$ 120万 - 项目类别:
Continuing Grant
相似海外基金
Exploring the periplasmic chaperone network underlying outer membrane protein folding
探索外膜蛋白折叠背后的周质伴侣网络
- 批准号:
22K05387 - 财政年份:2022
- 资助金额:
$ 120万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cell-type specific wiring of the chaperone network
伴侣网络的细胞类型特定布线
- 批准号:
RGPIN-2019-06230 - 财政年份:2022
- 资助金额:
$ 120万 - 项目类别:
Discovery Grants Program - Individual
Cell-type specific wiring of the chaperone network
伴侣网络的细胞类型特定布线
- 批准号:
RGPIN-2019-06230 - 财政年份:2021
- 资助金额:
$ 120万 - 项目类别:
Discovery Grants Program - Individual
Cell-type specific wiring of the chaperone network
伴侣网络的细胞类型特定布线
- 批准号:
RGPIN-2019-06230 - 财政年份:2020
- 资助金额:
$ 120万 - 项目类别:
Discovery Grants Program - Individual
Cell-type specific wiring of the chaperone network
伴侣网络的细胞类型特定布线
- 批准号:
DGECR-2019-00301 - 财政年份:2019
- 资助金额:
$ 120万 - 项目类别:
Discovery Launch Supplement
Structural basis for chaperone network mediated by semi-specific recognitions
半特异性识别介导的伴侣网络的结构基础
- 批准号:
19K06504 - 财政年份:2019
- 资助金额:
$ 120万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cell-type specific wiring of the chaperone network
伴侣网络的细胞类型特定布线
- 批准号:
RGPIN-2019-06230 - 财政年份:2019
- 资助金额:
$ 120万 - 项目类别:
Discovery Grants Program - Individual
Regulation of the BAG3 chaperone network under mechanical stress
机械应力下 BAG3 伴侣网络的调节
- 批准号:
401130824 - 财政年份:2018
- 资助金额:
$ 120万 - 项目类别:
Research Units
The prokaryotic Hsp90 chaperone network
原核 Hsp90 伴侣网络
- 批准号:
24580102 - 财政年份:2012
- 资助金额:
$ 120万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional characterization of the chaperone network connected to the eucaryotic ribosome
与真核核糖体连接的伴侣网络的功能表征
- 批准号:
64366273 - 财政年份:2008
- 资助金额:
$ 120万 - 项目类别:
Research Units