RAPID: Collaborative Research: Studies of recovery from bleaching in Acropora hyacinthus: epigenetic shifts, impacts on reproductive biology and carry-over effects

RAPID：合作研究：风信子卫城漂白恢复的研究：表观遗传变化、对生殖生物学的影响和遗留效应

• 批准号: 1935308
• 负责人: Marie Strader
• 金额: $ 7.61万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-15 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1935308&HistoricalAwards=false
• 关键词: RAPID; Collaborative; Research; Studies; recovery

Coral reefs provide strong economic and ecological benefits, yet they are declining worldwide largely due to extreme heat events that cause bleaching, a disturbance of the essential relationship between the algae that live inside the coral and the coral. There is currently a mass coral bleaching event in Moorea, French Polynesia where up to 90% of corals show some level of bleaching in response to heat stress. This location is ideal to study adaptation and acclimation thanks to the facilities and sampling of the Moorea Coral Reef (MCR) Long Term Ecological Research (LTER) site. This project explores how strong natural disaster events shape genetic differences in populations through time. By using historical environmental data it may be possible to identify modifications of the genome linked to past bleaching events. This knowledge will help establish models to predict reef recovery after disturbance and will be useful for choosing colonies with the best chance of survival in restoration efforts. This project also investigates how the bleaching history of the parents impacts characteristics of the next generations, such as reproductive output, larval, survival and heat tolerance. This project will provide training and involvement in research for three senior PhD students and at least five undergraduates. Coral restoration efforts rely on understanding how corals might adapt to environmental stress. The mass coral bleaching event currently occurring in French Polynesia (April 2019) offers an opportunity to test hypotheses regarding mechanisms of rapid response to large scale disturbances. This project investigates potential epigenetic and genetic mechanisms involved in either resisting stress or recovering from bleaching. The research leverages the Moorea Coral Reef (MCR) LTER, which integrates the high resolution oceanographic metrics and data on long-term community dynamics into the study of rapid adaptation of Acropora hyacinthus. Genetic and epigenetic signatures of a natural selection event (bleaching) are tracked in the field to test the impact of bleaching history on reproductive and carry-over effects in larval and juvenile corals. Both physiological and molecular methods, such as 2bRAD genotyping and reduced representation bisulfite sequencing, are employed to investigate correlations between phenotypes and genetic and epigenetic differences in the genome. This work explores associations between selection on genetic variation and epigenetic variation as well as the potential role of DNA methylation in phenotypic change across a generation in association with coral bleaching. In this era of global change, there is mounting evidence that rapid evolutionary processes are occurring at time scales relevant to ecological processes. Therefore, capitalizing on a system with rich long-term ecological data, such as that associated with the MCR LTER, is ideal to investigate mechanisms of rapid adaptation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

珊瑚礁提供了强大的经济和生态效益，但它们在世界范围内正在下降，主要是由于极端高温事件导致漂白，这扰乱了生活在珊瑚内的藻类与珊瑚之间的基本关系。目前，法属波利尼西亚的莫雷阿岛正在发生大规模珊瑚漂白事件，高达90%的珊瑚在热应激下表现出一定程度的漂白。这个位置是研究适应和驯化的理想场所，这要归功于莫雷阿珊瑚礁（MCR）长期生态研究（LTER）站点的设施和采样。该项目探讨了强烈的自然灾害事件如何通过时间塑造人群的遗传差异。通过使用历史环境数据，有可能确定与过去漂白事件有关的基因组修饰。这方面的知识将有助于建立模型来预测珊瑚礁恢复后的干扰，并将是有益的选择殖民地的最佳生存机会的恢复工作。该项目还调查了父母的漂白历史如何影响下一代的特征，如生殖输出，幼虫，存活和耐热性。该项目将为三名高年级博士生和至少五名本科生提供培训和参与研究。珊瑚恢复工作依赖于了解珊瑚如何适应环境压力。目前在法属波利尼西亚（2019年4月）发生的大规模珊瑚漂白事件提供了一个机会来测试有关对大规模干扰的快速反应机制的假设。该项目研究了潜在的表观遗传和遗传机制，涉及抵抗压力或从漂白中恢复。该研究利用了莫雷阿珊瑚礁（MCR）LTER，该系统将高分辨率海洋学指标和长期群落动态数据整合到Acropora hyacinthus快速适应的研究中。遗传和表观遗传签名的自然选择事件（漂白）在现场跟踪测试漂白历史的影响，在幼虫和少年珊瑚的繁殖和结转的影响。生理学和分子生物学方法，如2bRAD基因分型和减少代表性亚硫酸氢盐测序，研究表型和基因组中的遗传和表观遗传差异之间的相关性。这项工作探讨了遗传变异和表观遗传变异的选择之间的关联，以及DNA甲基化在一代人中与珊瑚漂白相关的表型变化中的潜在作用。在这个全球变化的时代，有越来越多的证据表明，快速的进化过程发生在与生态过程相关的时间尺度上。因此，利用MCR LTER等具有丰富长期生态数据的系统，是研究快速适应机制的理想选择。该奖项反映了NSF的法定使命，通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Nitrate enrichment has lineage specific effects on Pocillopora acuta adults, but no transgenerational effects in planulae

硝酸盐富集对尖球菌 (Pocillopora acuta) 成虫具有谱系特异性影响，但对浮浪藻没有跨代影响

• DOI: 10.1007/s00338-022-02236-9
• 发表时间: 2022
• 期刊: Coral Reefs
• 影响因子: 3.5
• 作者: Strader, Marie E.;Howe-Kerr, Lauren I.;Sims, Jordan A.;Speare, Kelly E.;Shore, Amanda N.;Burkepile, Deron E.;Correa, Adrienne M.
• 通讯作者: Correa, Adrienne M.

Reef habitats structure symbiotic microalgal assemblages in corals and contribute to differential heat stress responses

珊瑚礁栖息地在珊瑚中构建共生微藻群落，并导致不同的热应激反应

• DOI: 10.1007/s00338-022-02316-w
• 发表时间: 2023
• 期刊: Coral Reefs
• 影响因子: 3.5
• 作者: Leinbach, Sarah E.;Speare, Kelly E.;Strader, Marie E.
• 通讯作者: Strader, Marie E.

Size‐dependent mortality of corals during marine heatwave erodes recovery capacity of a coral reef

海洋热浪期间珊瑚的大小依赖性死亡率削弱了珊瑚礁的恢复能力

• DOI: 10.1111/gcb.16000
• 发表时间: 2021
• 期刊: Global Change Biology
• 影响因子: 11.6
• 作者: Speare, Kelly E.;Adam, Thomas C.;Winslow, Erin M.;Lenihan, Hunter S.;Burkepile, Deron E.
• 通讯作者: Burkepile, Deron E.