Mechanisms of zinc-sensitive metal-regulatory transcription factor 1 induction of hippocampal epileptic network activity (C03)

锌敏感金属调节转录因子1诱导海马癫痫网络活动的机制（C03）

• 批准号: 239935672
• 金额: --
• 依托单位: Institut für Neuropathologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/239935672?language=en
• 关键词: Mechanisms; zinc; sensitive; metal; regulatory

This project will focus on the mechanisms underlying the emergence of a hyperexcitable hippocampal network in temporal lobe epilepsy. The project has shown that a metal-regulatory transcription factor 1 (MTF1) is crucial in driving transformation of a subset of hippocampal neurons to bursting behavior via up-regulation of the CaV3.2 calcium channel. The project will use a combination of molecular, biochemical and electrophysiological approaches to determine the characteristics of Zn2+-sensitive MTF1-responsive neurons in epileptic network activity. It will develop a genetic label of neurons exhibiting MTF-1 activity, and then analyze the integration of this subset of cells into the circuit. In-vitro and in-vivo physiology and imaging will be used to determine how these cells are functionally integrated in epileptic networks.

本计画将著重于探讨颞叶癫痫中过度兴奋海马网络出现的机制。该项目已经表明，金属调节转录因子1（MTF 1）在通过上调CaV3.2钙通道驱动海马神经元子集转化为爆发行为方面至关重要。该项目将使用分子，生物化学和电生理方法的组合来确定癫痫网络活动中Zn 2+敏感的MTF 1反应神经元的特征。它将开发一种表现出MTF-1活性的神经元的遗传标签，然后分析这一子集的细胞整合到电路中。体外和体内生理学和成像将用于确定这些细胞在癫痫网络中的功能整合。