Analysis of the non-endosomal function of ESCRT-III proteins

ESCRT-III蛋白的非内体功能分析

• 批准号: 23998621
• 负责人: Professor Dr. Ralf Kölling
• 金额: --
• 依托单位: Fachgebiet Hefegenetik und Gärungstechnologie (150f)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/23998621?language=en
• 关键词: Analysis; non; endosomal; function; ESCRT

Internalized cell surface proteins pass through several endosomal compartments on their way to the lysosome/vacuole for degradation. Endocytic transport is mediated by a complex array of protein functions, many of which have been identified through genetic screens in the yeast Saccharomyces cerevisiae. Some of the proteins have been grouped into complexes ( ESCRT complexes ) that function consecutively at the level of late endosomes. One of these complexes, the ESCRT-III complex, is made up of a family of small coiled-coil forming proteins. Although all of these proteins function at a common step in the endocytic pathway, the corresponding mutants show distinct phenotypes. Genetic analyses suggest that these proteins have additional functions that are separate from their endosomal function. In this project, we will examine this non-endosomal function of the ESCRT-III proteins. We will focus on one member of this protein family, the Snf7 protein. In addition to its endosomal role, Snf7 affects the expression of genes in the nucleus. We will investigate how Snf7 interferes with gene expression in the nucleus.

内化的细胞表面蛋白在其到达溶酶体/液泡进行降解的途中穿过几个内体区室。内吞转运由一系列复杂的蛋白质功能介导，其中许多功能已通过酵母酿酒酵母中的基因筛选得到鉴定。一些蛋白质已被分组为复合物（ESCRT复合物），其在晚期内体水平连续发挥功能。这些复合物之一，ESCRT-III复合物，是由一个家庭的小卷曲螺旋形成蛋白质。尽管所有这些蛋白在内吞途径中的共同步骤中起作用，但相应的突变体显示出不同的表型。遗传分析表明，这些蛋白质具有与其内体功能分离的额外功能。在这个项目中，我们将研究ESCRT-III蛋白的非内体功能。我们将专注于这个蛋白质家族的一个成员，Snf 7蛋白。除了其内体作用外，Snf 7还影响细胞核中基因的表达。我们将研究Snf 7如何干扰细胞核中的基因表达。