NSFDEB-NERC: Collaborative research: Plant chemistry and its impact on diversification and habitat of plants adapted to extreme environments

NSFDEB-NERC：合作研究：植物化学及其对适应极端环境的植物多样化和栖息地的影响

• 批准号: 1939226
• 负责人: Ya Yang
• 金额: $ 48.22万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2025-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1939226&HistoricalAwards=false
• 关键词: NSFDEB; NERC; Collaborative; research; Plant

Plants produce diverse chemical compounds, including pigments for attracting pollinators and toxins for deterring herbivores. These chemical compounds are often unique to certain plant parts in different plant groups. This project seeks to understand the evolution of plant-derived chemical compounds and how these compounds promote plant diversity. To address these questions, the project will focus on the plants within the Caryophyllales, a group that includes cacti and many alpine and arctic plants known for extraordinary adaptations to warm, dry, or cold environments. Many Caryophyllales species produce unusually large amounts of diverse compounds derived from the amino acid tyrosine. Due to inherent biochemical trade-offs, the production of these tyrosine-derived compounds may come at the expense of other compounds derived from the amino acid phenylalanine. We will study the balance of the production of these two amino acids and its effect on the production of myriad compounds important in UV shielding, pollination, and herbivore deterrence. The work will inform the biotechnological production of natural plant pigments and will help to elucidate biosynthetic pathways of a number of high-value plant-derived chemicals with pharmaceutical value. The collaborative US-UK team will provide multidisciplinary training opportunities for students and post-doctoral scholars in genetic and biochemical methods in an evolutionary context. Workshops at professional society meetings, a K-12 summer camp module, and multiple public outreach modules will bring these concepts to the public and highlight the relationship between biodiversity and high-value plant chemical compounds. This study will perform an extensive survey to establish the occurrence and distribution of key tyrosine- and phenylalanine-derived metabolites in 600 species across Caryophyllales; examine the association of these tyrosine-derived metabolic traits with organismal adaptation and diversification patterns; and determine the evolutionary genetic mechanisms responsible for the biosynthesis of these metabolites. New tools will be developed to model the hierarchical nature of metabolite evolution and will be made available to the broader community of scientists. This project will comprehensively describe the extent of tyrosine-enriched metabolism in Caryophyllales, define the degree to which these metabolites are associated with organismal diversification patterns across Caryophyllales, and resolve the evolutionary assembly of the genetic pathways underlying complex tyrosine-derived metabolic traits. This award was co-funded by the Systematics and Biodiversity Science Cluster in the Division of Environmental Biology and the Integrative Ecological Physiology Program in the Division of Integrative Organismal Systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物产生各种各样的化合物，包括吸引传粉者的色素和阻止食草动物的毒素。这些化合物通常是不同植物群中某些植物部位所特有的。该项目旨在了解植物衍生化合物的演变以及这些化合物如何促进植物多样性。为了解决这些问题，该项目将重点关注Carlellales内的植物，这是一个包括仙人掌和许多高山和北极植物的群体，这些植物以适应温暖，干燥或寒冷的环境而闻名。许多卡氏藻目物种产生异常大量的衍生自氨基酸酪氨酸的不同化合物。由于固有的生物化学权衡，这些酪氨酸衍生的化合物的产生可能以衍生自氨基酸苯丙氨酸的其他化合物为代价。我们将研究这两种氨基酸生产的平衡及其对紫外线屏蔽，授粉和食草动物威慑中重要的无数化合物生产的影响。这项工作将为天然植物色素的生物技术生产提供信息，并将有助于阐明一些具有药用价值的高价值植物衍生化学品的生物合成途径。合作的美英团队将为学生和博士后学者提供多学科的培训机会，在进化背景下的遗传和生物化学方法。专业协会会议上的研讨会，K-12夏令营模块和多个公共宣传模块将把这些概念带给公众，并强调生物多样性和高价值植物化合物之间的关系。这项研究将进行广泛的调查，以建立关键的酪氨酸和苯丙氨酸衍生的代谢产物的发生和分布在600个物种在Carbellales;检查这些酪氨酸衍生的代谢性状与生物体适应和多样化模式的关联;并确定负责这些代谢产物的生物合成的进化遗传机制。将开发新的工具来模拟代谢物演变的等级性质，并将提供给更广泛的科学界。该项目将全面描述酪氨酸富集代谢的程度，在Carbellales，定义这些代谢产物与生物多样性模式在Carbellales的程度，并解决复杂的酪氨酸衍生的代谢特征的遗传途径的进化组装。该奖项由环境生物学部的系统学和生物多样性科学组和综合有机体系统部的综合生态生理学项目共同资助。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Are seven amino acid substitutions sufficient to explain the evolution of high l ‐DOPA 4,5‐dioxygenase activity leading to betalain pigmentation? Revisiting the gain‐of‐function mutants of Bean et al . (2018)

七个氨基酸取代是否足以解释导致甜菜红素色素沉着的高 l -多巴 4,5 - 双加氧酶活性的演变？

• DOI: 10.1111/nph.18981
• 发表时间: 2023
• 期刊: New Phytologist
• 影响因子: 9.4
• 作者: Guerrero‐Rubio, M. Alejandra;Walker‐Hale, Nathanael;Guo, Rui;Sheehan, Hester;Timoneda, Alfonso;Gandia‐Herrero, Fernando;Brockington, Samuel F.
• 通讯作者: Brockington, Samuel F.

Traditional medicinal use is linked with apparency, not specialized metabolite profiles in the order Caryophyllales

• DOI: 10.1002/ajb2.16308
• 发表时间: 2024-04-05
• 期刊: AMERICAN JOURNAL OF BOTANY
• 影响因子: 3
• 作者: Crum，Alex H.;Philander，Lisa;Yang，Ya
• 通讯作者: Yang，Ya