权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Establishment and maintenance of repressive chromatin during development in plants

植物发育过程中抑制性染色质的建立和维持

基本信息

批准号：
1951698
负责人：
Joseph Ogas
金额：
$ 91.77万
依托单位：
Purdue University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-03-01 至 2024-02-29
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1951698&HistoricalAwards=false
关键词：
Establishment maintenance repressive chromatin during

项目摘要

This project will study how plants control expression of specific genes during development by changing how the genes are packaged in the nucleus. In plants, like in other eukaryotic organisms, the very large genome is packaged into the small space of the cell's nucleus by winding the genomic DNA around proteins to form a compacted DNA-protein complex called chromatin. Very tightly compacted chromatin can prevent gene expression by mechanisms that are not fully understood. Exploring these mechanisms is the focus of this proposal. A long-term goal will be to use the research outcomes to manipulate development in plants to increase their agricultural potential. The research will be carried out by undergraduate and graduate students working together, providing an authentic research experience for undergraduate students and mentoring opportunities for the graduate students. In addition, analysis of the genomic data generated in the project will be incorporated into a new computational genomics course to teach students how to analyze large datasets using computational approaches. All of the data generated by the project will be deposited in public databases.In eukaryotes, including the model plant Arabidopsis thaliana, trimethylation of lysine 27 on histone H3 (H3K27me3) in nucleosomal chromatin is associated with transcriptional repression, but how this histone modification is imposed, maintained, and read is poorly understood. The chromatin remodeler named PICKLE (PKL) has been shown previously to promote tissue- and developmental-specific repressive chromatin, defined by the presence of H3K27me3. Recent evidence suggests that PKL may function as a pre-nucleosome maturation factor that acts with a histone methyltransferase, a second remodeler, and a histone H2A variant to control conditional expression from H3K27me3-enriched loci. This relationship will be tested by combining genetic, genomic, and biochemical approaches to relate chromatin-mediated changes in gene expression to whole-plant developmental phenotypes. The results are expected to reveal the role of H3K27me3 homeostasis in formation and maintenance of repressive chromatin states.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个项目将研究植物在发育过程中如何通过改变基因在细胞核中的包装方式来控制特定基因的表达。在植物中，就像在其他真核生物中一样，通过将基因组DNA缠绕在蛋白质周围形成紧凑的DNA-蛋白质复合体，非常大的基因组被包装到细胞核的小空间中，称为染色质。非常紧密的染色质可以通过尚未完全了解的机制来阻止基因表达。探索这些机制是这项提案的重点。一个长期目标将是利用研究成果来操纵植物的发展，以增加它们的农业潜力。研究将由本科生和研究生共同开展，为本科生提供真实的研究体验，为研究生提供指导机会。此外，对项目中产生的基因组数据的分析将被纳入一门新的计算基因组学课程，教会学生如何使用计算方法分析大型数据集。该项目产生的所有数据都将存储在公共数据库中。在真核生物中，包括模式植物拟南芥，核小体染色质中组蛋白H3(H3K27me3)上赖氨酸27的三甲基化与转录抑制有关，但这种组蛋白修饰是如何施加、维持和阅读的，人们知之甚少。之前已经证明，名为PICLE(PKL)的染色质重构体可以促进组织和发育特异的抑制性染色质，由H3K27me3的存在定义。最近的证据表明，PKL可能是一个核小体成熟前因子，它与组蛋白甲基转移酶、第二重构体和组蛋白H_2A变异体共同作用，控制H3K27me3富集区的条件表达。这种关系将通过结合遗传、基因组和生化方法来测试，以将染色质介导的基因表达变化与整个植物的发育表型联系起来。这一结果有望揭示H3K27me3在抑制染色质状态的形成和维持中的作用。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。