ARTS: Uncovering the Desert Darkling Beetles (Tenebrionidae: Pimeliinae): Phylogenomics, phenomics, and biogeography in a megadiverse global radiation

艺术：揭示沙漠暗甲虫（拟甲虫科：Pimeliinae）：全球辐射多样性中的系统基因组学、表型组学和生物地理学

• 批准号: 2009247
• 负责人: Aaron Smith
• 金额: $ 53.53万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-10-24 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2009247&HistoricalAwards=false
• 关键词: ARTS; Uncovering; Desert; Darkling; Beetles

Desert darkling beetles (Coleoptera: Tenebrionidae: Pimeliinae) are an incredibly diverse (8,000+ described species) but poorly studied group of organisms that are found across the world, particularly in desert environments. Indeed, many species have evolved to survive in some of the driest and hottest places on Earth, and form a key component of desert ecosystems. The subfamily includes well known species such as Namibian fog basking beetles (Onymacris unguicularis, used in biomimicry research) and the North American blue death-feigning beetle (Asbolus verrucosus), along with thousands of poorly known or still undescribed species. This research will describe hundreds of species new to science, explore where these beetles live and how they are related to each other across the planet, and investigate the various adaptations that have allowed these beetles to thrive and diversify across the world's deserts. This project will also train a postdoctoral researcher, two Ph.D. students, and approximately 15 undergraduate students, as well as facilitating collaborations and providing training to a diverse group of scientists from across the globe. Project participants will also be designing and leading hybrid courses between Northern Arizona University and Namibia's renowned Gobabeb Research Center, and working to enhance research collections in all participating institutions. This project will resolve centuries-old issues in our understanding of desert darkling beetles by 1) generating DNA data from recently collected and museum specimens using high-throughput sequencing and target enrichment methods to recover ~631 genetic loci for approximately 500 species across the Pimeliinae. Additionally, approximately 300 larval and adult morphological characters will be scored for each species. Morphological data will be combined with the molecular data to reconstruct the evolutionary history of the subfamily. The resulting phylogeny will inform taxonomic decisions and help place current distributions and life histories within an evolutionary context. 2) Producing an Open Access electronic monograph of the subfamily using the mx/TaxonWorks bioinformatics platform and a matrix-based approach to coding and organizing morphological characters for descriptions, linked to the Coleoptera Anatomy Ontology (ColAO) to provide explicit definitions and images for anatomical terms. The monograph will include the first worldwide key to the Pimeliinae tribes and subtribes, images of specimens and characters, distribution maps, an extensive catalog of darkling beetle names and literature, and matrix-based descriptions. 3) Examining historical biogeography, diversification patterns, and adaptations to xeric conditions across the subfamily using the resulting phylogeny, over 20,000 georeferenced museum and modern specimens, and recently developed analytical tools for biogeographic analyses.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

沙漠暗甲虫（鞘翅目：拟步甲科：Pimeliinae）是一个令人难以置信的多样性（8,000+描述的物种），但研究很少的生物群体，在世界各地发现，特别是在沙漠环境中。事实上，许多物种已经进化到在地球上一些最干燥和最热的地方生存，并形成了沙漠生态系统的关键组成部分。该亚科包括众所周知的物种，如纳米比亚雾晒甲虫（Onymacris unguicularis，用于仿生学研究）和北美蓝色假死甲虫（Asbolus verrucosus），沿着数千种鲜为人知或尚未描述的物种。这项研究将描述数百种新的科学物种，探索这些甲虫生活在哪里以及它们在地球上如何相互关联，并调查使这些甲虫在世界沙漠中茁壮成长和多样化的各种适应。本项目还将培养一名博士后研究员，两名博士研究生。该项目的主要目标是促进合作，并为来自地球仪的不同科学家群体提供培训。项目参与者还将设计和领导北方亚利桑那大学和纳米比亚著名的Gobabeb研究中心之间的混合课程，并努力加强所有参与机构的研究收藏。该项目将通过以下方式解决我们对沙漠暗甲虫的理解中存在的几个世纪的问题：1）使用高通量测序和目标富集方法从最近收集的和博物馆标本中生成DNA数据，以恢复Pimeliinae中约500个物种的约631个遗传位点。此外，将对每个种属的约300个幼虫和成虫形态特征进行评分。形态学数据将与分子数据相结合，重建亚科的进化历史。由此产生的遗传学将为分类决策提供信息，并有助于将当前的分布和生活史置于进化背景下。2)使用mx/TaxonWorks生物信息学平台和基于矩阵的方法来编码和组织描述的形态特征，与鞘翅目解剖学本体（ColAO）相关联，以提供解剖术语的明确定义和图像，制作该亚科的开放获取电子专著。该专著将包括世界上第一个Pimeliinae部落和亚部落的钥匙、标本和特征的图像、分布图、广泛的暗甲虫名称和文献目录以及基于矩阵的描述。3)研究历史地理学，多样化模式，以及整个亚科对干旱条件的适应性，使用由此产生的遗传学，超过20，000个地理参考博物馆和现代标本，以及最近开发的地理分析工具进行地理分析。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。

项目成果

ILLUSTRATED CATALOG OF THE SUBTRIBE HELOPININA LACORDAIRE (COLEOPTERA: TENEBRIONIDAE: BLAPTINAE: PEDININI)

• DOI: 10.1649/0010-065x-75.3.537
• 发表时间: 2021-09-01
• 期刊: COLEOPTERISTS BULLETIN
• 影响因子: 0.7
• 作者: Kaminski, Marcin Jan;Lumen, Ryan;Smith, Aaron Dennis
• 通讯作者: Smith, Aaron Dennis

Formalizing Invertebrate Morphological Data: A Descriptive Model for Cuticle-Based Skeleto-Muscular Systems, an Ontology for Insect Anatomy, and their Potential Applications in Biodiversity Research and Informatics

• DOI: 10.1093/sysbio/syad025
• 发表时间: 2023-04-24
• 期刊: SYSTEMATIC BIOLOGY
• 影响因子: 6.5
• 作者: Giron，Jennifer C.;Tarasov，Sergei;Miko，Istvan
• 通讯作者: Miko，Istvan

On the Taxonomic Placement of Penichrus Champion, 1885 and a Synopsis of North American Opatrini (Coleoptera: Tenebrionidae: Blaptinae)

关于 Penichrus Champion 的分类学定位，1885 年和北美 Opatrini 概要（鞘翅目：拟步甲科：Blaptinae）

• DOI: 10.3161/00034541anz2020.70.4.017
• 发表时间: 2020
• 期刊: Annales Zoologici
• 影响因子: 1.1
• 作者: Johnston, M. Andrew;Smith, Aaron D.;Matsumoto, Keita;Kamiński, Marcin Jan
• 通讯作者: Kamiński, Marcin Jan

TESTING THE TAXONOMY OF AMPHIDORINI LECONTE (COLEOPTERA: TENEBRIONIDAE): A MOLECULAR PHYLOGENY LEVERAGING MUSEUM SEQUENCING

• DOI: 10.3161/00034541anz2022.72.1.003
• 发表时间: 2022-03-01
• 期刊: ANNALES ZOOLOGICI
• 影响因子: 1.1
• 作者: Johnston，M. Andrew;Smith，Aaron D.;Franz，Nico M.
• 通讯作者: Franz，Nico M.

The Future for a Prominent Taxonomy

杰出分类学的未来

• DOI: 10.1093/isd/ixaa020
• 发表时间: 2021
• 期刊: Insect Systematics and Diversity
• 影响因子: 3.4
• 作者: Hamilton, Chris A;Shockley, Floyd W;Simmons, Rebecca;Smith, Aaron;Ware, Jessica;Zaspel, Jennifer M
• 通讯作者: Zaspel, Jennifer M