NSF Postdoctoral Fellowship in Biology FY 2020: Investigating maternally-mediated developmental plasticity and the role of the microbiome in wild red squirrels

2020 财年 NSF 生物学博士后奖学金：研究母源介导的发育可塑性和微生物组在野生红松鼠中的作用

• 批准号: 2010726
• 负责人: Lauren Petrullo
• 金额: $ 13.8万
• 依托单位: Petrullo, Lauren
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2010726&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; FY

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2020, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of Life in innovative ways. This project aims to identify how the microbiome—the community of bacteria living in and on a host—connects ecological change and developmental differences in wild red squirrels. An individual’s microbiome is inherited in part through maternally-transmitted microbes and can shift in response to environmental changes. How do these shifts in the microbiome relate to variation in development, and are these changes an advantage or disadvantage? The fellow will address these questions using long-term data collected on wild red squirrels living in the Yukon, Canada. Red squirrels are highly territorial and experience exreme fluctuations in food availability, making them an excellent model to examine how the microbiome responds to ecological and social change. This research will provide critical insight into how microbes contribute to the evolutionary processes that allow animals to adapt to changing environments. The fellow will include first-generation and underrepresented students in various aspects of this project and disseminate primary research findings through various existing institutional mechanisms aimed at engaging local communities in the scientific process. This research synthesizes theory and methods from multiple fields, combining high-resolution genomics with noninvasive hormone analyses while situating the data within an eco-evolutionary framework. This study will use experimental manipulations of population density and food availability to 1) probe interactions between hormonal responses to ecological change and the microbiome, 2) investigate the vertical transmission of microbes from mothers to offspring, and 3) test whether microbial changes predict variation in offspring growth rates. This project will then assess the adaptive value of microbial changes by 4) testing how the microbiome relates to territory acquisition, survival to first breeding, and longevity using both existing longitudinal data and a novel cross-fostering experiment. The fellow will receive integrative training across the fields of genomics, endocrinology, evolutionary biology, and ecology and data will be generated and archived for maximum reproducibility and accessibility. Underserved communities and first-generation students will be engaged in this research through hands-on mini-projects, institutional mentorship programs, and a citizen science community outreach program on red squirrels.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项行动资助了2020财年的NSF生物学博士后研究奖学金，综合研究调查了基因组，环境和表型之间的生命相互作用的规则。该研究金支持研究员的研究和培训，以创新的方式为生活规则领域做出贡献。该项目旨在确定微生物组-生活在宿主体内和宿主上的细菌群落-如何连接野生红松鼠的生态变化和发育差异。一个人的微生物组部分是通过母体传播的微生物遗传的，并且可以根据环境变化而改变。微生物组的这些变化与发育的变化有何关系？这些变化是优势还是劣势？该研究员将使用长期收集的生活在加拿大育空地区的野生红松鼠的数据来解决这些问题。红松鼠具有高度的领土性，并且在食物供应方面经历了极大的波动，这使它们成为研究微生物组如何响应生态和社会变化的绝佳模型。这项研究将为微生物如何促进动物适应不断变化的环境的进化过程提供重要的见解。该研究员将让第一代和代表性不足的学生参与该项目的各个方面，并通过各种现有体制机制传播主要研究成果，以使当地社区参与科学进程。 这项研究综合了多个领域的理论和方法，将高分辨率基因组学与非侵入性激素分析相结合，同时将数据置于生态进化框架内。这项研究将使用人口密度和食物可用性的实验操作来1）探索激素对生态变化的反应与微生物组之间的相互作用，2）调查微生物从母亲到后代的垂直传播，3）测试微生物变化是否预测后代生长率的变化。然后，该项目将评估微生物变化的适应价值，方法是4）使用现有的纵向数据和一种新的交叉培养实验来测试微生物组与领土获取、首次繁殖的生存和寿命的关系。该研究员将接受基因组学，内分泌学，进化生物学和生态学领域的综合培训，并将生成和存档数据，以实现最大的可重复性和可访问性。服务水平低下的社区和第一代学生将通过实践小型项目、机构指导计划和关于红松鼠的公民科学社区外展计划参与这项研究。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

The glucocorticoid response to environmental change is not specific to agents of natural selection in wild red squirrels

糖皮质激素对环境变化的反应并非野生红松鼠自然选择因子所特有的

• DOI: 10.1016/j.yhbeh.2022.105262
• 发表时间: 2022
• 期刊: Hormones and Behavior
• 影响因子: 3.5
• 作者: Petrullo, Lauren;Delaney, David;Boutin, Stan;McAdam, Andrew G.;Lane, Jeffrey E.;Boonstra, Rudy;Palme, Rupert;Dantzer, Ben
• 通讯作者: Dantzer, Ben

Phenotype–environment mismatch errors enhance lifetime fitness in wild red squirrels

表型与环境不匹配错误增强了野生红松鼠的终生适应性

• DOI: 10.1126/science.abn0665
• 发表时间: 2023
• 期刊: Science
• 影响因子: 56.9
• 作者: Petrullo, Lauren;Boutin, Stan;Lane, Jeffrey E.;McAdam, Andrew G.;Dantzer, Ben
• 通讯作者: Dantzer, Ben