NSF Postdoctoral Fellowship in Biology FY 2020: Friend or foe: Neural regulation of dynamic social environments

2020 财年 NSF 生物学博士后奖学金：朋友还是敌人：动态社会环境的神经调节

• 批准号: 2010906
• 负责人: Ross DeAngelis
• 金额: $ 13.8万
• 依托单位: DeAngelis Ross S
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-11-01 至 2023-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2010906&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; FY

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2020, Integrative Research Investigating the Rules of Life Governing Interactions between Genomes, Environment and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of life in innovative ways. Social behavior is complex, individuals must use external information from a continually changing environment in order to display appropriate behavioral responses. The external social information is then applied within the current social context. Behavioral responses are thus dependent on the situation. This response dependency must also be reflected by predictable patterns within the brain. This research aims to uncover how neural (nervous system) activity orchestrates behavior in a socially and environmentally sensitive way. This work will broaden our understanding of how fundamental social behaviors are coordinated within the brain, while also providing opportunities in research training for undergraduates and high school students of underrepresented minorities.This research utilizes the convict cichlid (Amatitlania nigrofasciata) as a study system to establish the rule of life that social behavior is facilitated by predictable interactions of brain areas within the Social Decision-Making Network, specifically those sensitive to social information and those encoding reward. The convict cichlid is ideally suited to address this hypothesis, as this species displays pair bonding and parental care, two behavioral suites which contain both aggressive and affiliative elements. Parents both provision and guard eggs, and mated pairs reinforce their bond through affiliative displays, while also guarding mates against conspecifics. How are both distinct and shared behavioral displays, occurring within and across behavioral suites, regulated within the brain? This research implements compartment analysis of temporal activity by fluorescent in situ hybridization (catFISH) to compare the neuronal response of temporally isolated social stimulus events across four comparisons, within and across behavioral suites: (1a.) parental care (brood provision vs. brood guarding) and 1b.) pair bonding (mate affiliation vs. mate guarding), and second, compares similar behavioral displays, which occur in distinct behavioral suites (similar behavioral displays). 1c.) Brood provisioning vs. mate affiliation (affiliative behavior), and (1d.) brood guarding vs. mate guarding (aggressive behavior). Finally, brain regions will be dissected out and behaviorally active neurons will be tagged and selectively retrieved using ribosomal capture (PhosphoTRAP), in order to uncover the genomic signatures of neurons active in response to each independent stimulus. Hence, through integrating neural circuitry and genomics, this work will further our understating of brain-behavior relationships. The impact of the project will be broadened by involving high school and undergraduate students from underrepresented groups in the research.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动资助了2020财年NSF生物学博士后研究奖学金，综合研究调查了基因组，环境和表型之间的生命管理相互作用的规则。该研究金支持研究员的研究和培训，以创新的方式为生活规则领域做出贡献。社会行为是复杂的，个体必须利用来自不断变化的环境的外部信息，才能表现出适当的行为反应。然后，外部社会信息被应用于当前社会背景中。因此，行为反应取决于情境。这种反应依赖性也必须通过大脑中可预测的模式来反映。这项研究旨在揭示神经（神经系统）活动如何以社会和环境敏感的方式协调行为。这项工作将拓宽我们对基本社会行为如何在大脑中协调的理解，同时也为代表性不足的少数民族的本科生和高中生提供研究培训的机会。（Amatitlania nigrofasciata）作为一个研究系统，以建立生活规则，即社会行为是由社会决策中大脑区域的可预测相互作用促进的，制造网络，特别是那些对社会信息敏感的人和那些编码奖励的人。定罪丽鱼是理想的适合解决这一假设，因为这个物种显示对债券和父母的照顾，两个行为套件，其中包含侵略和亲和元素。父母双方都提供和保护鸡蛋，交配对加强他们的债券通过附属显示，同时也保护配偶对同种。在大脑中，不同的和共享的行为展示是如何在行为套件内和跨行为套件发生的？本研究通过荧光原位杂交（catFISH）实现时间活动的区室分析，以在行为套件内和跨行为套件的四个比较中比较时间隔离的社会刺激事件的神经元反应：（1a）父母照顾（育雏规定与育雏监护）和1b.）第二，比较发生在不同行为套件中的相似行为展示（相似行为展示）。1c.）育雏供应与配偶关系（亲和行为），和（1d.）护雏与配偶保护（攻击行为）。最后，大脑区域将被解剖出来，行为活跃的神经元将被标记并使用核糖体捕获（PhosphoTRAP）选择性地检索，以揭示对每个独立刺激做出反应的神经元的基因组特征。因此，通过整合神经回路和基因组学，这项工作将进一步加深我们对大脑-行为关系的理解。该项目的影响将通过让来自代表性不足群体的高中生和本科生参与研究来扩大。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。