SBIR Phase I: Novel drug delivery system using engineered exosomes

SBIR 第一阶段：使用工程外泌体的新型药物递送系统

• 批准号: 2014266
• 负责人: Souvenir Tachado
• 金额: $ 22.5万
• 依托单位: SOUVIE BIODELIVERY LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-15 至 2022-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2014266&HistoricalAwards=false
• 关键词: SBIR; Phase; Novel; drug; delivery

he broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase 1 project entails developing an novel drug delivery platform to deliver drug into poorly accessible druggable targets. Drugs targeting inflammation are encapsulated in an new way for treatment of chronic inflammatory diseases such as psoriasis, lupus, rheumatoid arthritis. Biological therapies remain the standard for autoimmune diseases, but patients can have immune system problems due to the production of autoantibodies. This project addresses this challenge with a novel encapsulation technology that delivers biological therapies to hard-to-reach inflammation sites. This technology could be advantageous because it reduces immunosuppression and anti-drug antibodies production. This project will improve patient treatment outcomes and reduce treatment reduce cost. This Small Business Innovation Research (SBIR) Phase 1 project will test a proof-of-concept that entails demonstrating that an exosome-based delivery vehicle for biologics will potentially inhibit inappropriate TLR-driven inflammation. Induction of autoimmune diseases including psoriasis, rheumatoid arthritis and lupus strongly correlate with chronic inflammation driven by inflammatory signaling pathways mediated by endosomal TLRs. Our goal is to develop an exosome-based therapy that can inhibit poorly accessible endosomal TLR7-driven inflammation. To test this concept, our plan involves genetically modifying human cell lines to produce engineered exosomes that contain anti-TLR7 antibody to inhibit endosomal TLR7-driven inflammation. We will develop a drug encapsulation platform in which engineered exosomes bear an anti-TLR7 antibody fused with an exosome transmembrane protein called tetraspanin. This complex delivers an antibody payload to TLR7 targets located in on endosomal membrane inner surface. This anti-TLR7 antibody will bind to dimer-interfaces of TLR7 monomer and thereby block TLR7 receptor dimerization, which in turn blocks proinflammatory signaling. This work is important because blocking TLR7 receptor activation, which is situated upstream in the signaling cascade, will presumably diminish immunosuppression and lead to a better outcome that current anti-TNF therapies that are more broad spectrum because their site of action is downstream in a signaling pathway axis.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个小企业创新研究（SBIR）第1阶段项目的更广泛的影响/商业潜力需要开发一种新的药物输送平台，将药物输送到难以到达的可药用目标。针对炎症的药物以新的方式被封装，用于治疗慢性炎症性疾病，如银屑病、狼疮、类风湿性关节炎。生物疗法仍然是自身免疫性疾病的标准疗法，但由于自身抗体的产生，患者可能会出现免疫系统问题。该项目通过一种新的封装技术解决了这一挑战，该技术可将生物疗法提供给难以到达的炎症部位。这项技术可能是有利的，因为它减少了免疫抑制和抗药抗体的产生。该项目将改善患者的治疗效果，减少治疗费用。这个小企业创新研究（SBIR）第1阶段项目将测试一个概念验证，该项目需要证明基于外泌体的生物制剂递送载体可能会抑制不适当的TLR驱动的炎症。包括银屑病、类风湿性关节炎和狼疮在内的自身免疫性疾病的诱导与由内体TLR介导的炎性信号传导途径驱动的慢性炎症密切相关。我们的目标是开发一种基于外泌体的疗法，可以抑制难以进入的内体TLR 7驱动的炎症。为了测试这一概念，我们的计划涉及对人类细胞系进行遗传修饰，以产生含有抗TLR 7抗体的工程外泌体，以抑制内体TLR 7驱动的炎症。我们将开发一种药物包封平台，其中工程化外泌体携带抗TLR 7抗体，该抗体与称为tetraspanin的外泌体跨膜蛋白融合。该复合物将抗体有效载荷递送至位于内体膜内表面上的TLR 7靶标。这种抗TLR 7抗体将结合TLR 7单体的二聚体界面，从而阻断TLR 7受体二聚化，这进而阻断促炎信号传导。这项工作是重要的，因为阻断TLR 7受体激活，这是位于上游的信号级联反应，可能会减少免疫抑制，并导致更好的结果，目前的抗该奖项反映了NSF的法定使命，并通过使用基金会的知识产权进行评估，被认为值得支持。优点和更广泛的影响审查标准。