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Collaborative Research: Mitigating antibiotic resistance in drinking water by understanding the impact of corrosion inhibitors and corrosion products

合作研究：通过了解腐蚀抑制剂和腐蚀产物的影响来减轻饮用水中的抗生素耐药性

基本信息

批准号：
2027233
负责人：
Yin Wang
金额：
$ 20.37万
依托单位：
University of Wisconsin-Milwaukee
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-15 至 2024-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027233&HistoricalAwards=false
关键词：
Collaborative Research Mitigating antibiotic resistance

项目摘要

The spread of antibiotic resistance threatens public health by rendering antibiotics ineffective. Drinking water distribution systems are a source of antibiotic resistance genes, the genetic material that makes bacteria resistant to antibiotics. Water distribution systems are particularly concerning as they are a direct conveyor of antibiotic resistance genes to vulnerable populations including children, the elderly, hospital patients, and people with weakened immune systems. Metals are known to promote the development of antibiotic resistance. Some of these metals are also present in distribution systems due to pipe corrosion and the presence of corrosion inhibitors. The goal of this research is to determine which water pipe materials and corrosion inhibitor choices result in minimal antibiotic resistance. This goal will be achieved through experiments to examine the role of corrosion on antibiotic resistance development. Collaborations between the research team and water utilities will facilitate research to determine the interplay between resistance genes, pipe surfaces, and drinking water chemistry in real-world distribution systems. Further benefits to society will result from guidance provided to drinking water utilities across the country. This guidance will help them decide which pipe materials and corrosion inhibitors best prevent the spread of antibiotic resistance in drinking water to protect public health. The spread of antibiotic resistance is a primary public health concern. Metals are a known stressor for antibiotic resistance and are prevalent in drinking water systems due to aging infrastructure and the use of metal-containing corrosion inhibitors. The central hypothesis of this work is that corrosion inhibitors and corrosion products yield metals in water distribution systems that increase the abundance and alter the types of antibiotic resistant genes in municipal drinking water. The research team combines expertise in chemistry and molecular microbiology to address three complimentary objectives. Objective 1 is to determine the impact of corrosion inhibitors on antibiotic resistance under variable nutrient conditions. Objective 2 is to elucidate the effect of corrosion products on antibiotic resistance using complimentary culture-based and non-culture-based techniques. Objective 3 is to identify linkages between corrosion products and the quantity and profile of antibiotic resistance genes in real-world drinking water distribution system pipes. Surface chemistry characterization will be coupled with Droplet Digital PCR to identify chemical relationships to antibiotic resistance gene profiles. Knowledge generated from this research will help utilities mitigate the spread of antibiotic resistance in drinking water distribution systems. Results will be incorporated into an annual two-day professional short course on emerging contaminants in water and wastewater. Additionally, the PIs will build on previous summer outreach efforts and host a high school course to engage underrepresented students in summer research to increase the diversity of the Nation’s STEM workforce.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

抗生素耐药性的蔓延使抗生素失效，从而威胁公众健康。饮用水分配系统是抗生素抗性基因的来源，抗生素抗性基因是使细菌对抗生素产生抗性的遗传物质。供水系统尤其令人担忧，因为它们是将抗生素抗性基因直接传送给弱势群体，包括儿童、老年人、医院病人和免疫系统较弱的人。众所周知，金属会促进抗生素耐药性的发展。由于管道腐蚀和腐蚀抑制剂的存在，其中一些金属也存在于分配系统中。这项研究的目的是确定哪些水管材料和腐蚀抑制剂的选择可以将抗生素耐药性降至最低。这一目标将通过实验来检验腐蚀对抗生素耐药性发展的作用来实现。研究团队和自来水公司之间的合作将促进研究，以确定现实世界的分配系统中抗性基因、管道表面和饮用水化学之间的相互作用。为全国饮用水公用事业提供的指导将为社会带来进一步的好处。该指南将帮助他们决定哪种管道材料和腐蚀抑制剂最能防止饮用水中抗生素耐药性的蔓延，以保护公众健康。抗生素耐药性的蔓延是一个主要的公共卫生问题。金属是已知的抗生素耐药性压力源，由于基础设施老化和含金属腐蚀抑制剂的使用，金属在饮用水系统中普遍存在。这项工作的中心假设是，腐蚀抑制剂和腐蚀产物在配水系统中产生金属，从而增加城市饮用水中抗生素抗性基因的丰度并改变其类型。该研究团队结合了化学和分子微生物学方面的专业知识来实现三个互补的目标。目标 1 是确定腐蚀抑制剂在可变营养条件下对抗生素耐药性的影响。目标 2 是利用基于培养和非培养的互补技术阐明腐蚀产物对抗生素耐药性的影响。目标 3 是确定腐蚀产物与现实饮用水分配系统管道中抗生素抗性基因的数量和概况之间的联系。表面化学表征将与微滴数字 PCR 相结合，以确定与抗生素抗性基因谱的化学关系。这项研究产生的知识将帮助公用事业公司减轻饮用水分配系统中抗生素耐药性的蔓延。结果将纳入每年为期两天的关于水和废水中新兴污染物的专业短期课程。此外，PI 将在之前的夏季推广工作的基础上举办一门高中课程，让代表性不足的学生参与夏季研究，以增加国家 STEM 劳动力的多样性。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。