Collaborative Research: Mitigating antibiotic resistance in drinking water by understanding the impact of corrosion inhibitors and corrosion products

合作研究：通过了解腐蚀抑制剂和腐蚀产物的影响来减轻饮用水中的抗生素耐药性

基本信息

批准号：
2027288
负责人：
Patrick McNamara
金额：
$ 21.63万
依托单位：
Marquette University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-15 至 2024-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027288&HistoricalAwards=false
关键词：
Collaborative Research Mitigating antibiotic resistance

项目摘要

The spread of antibiotic resistance threatens public health by rendering antibiotics ineffective. Drinking water distribution systems are a source of antibiotic resistance genes, the genetic material that makes bacteria resistant to antibiotics. Water distribution systems are particularly concerning as they are a direct conveyor of antibiotic resistance genes to vulnerable populations including children, the elderly, hospital patients, and people with weakened immune systems. Metals are known to promote the development of antibiotic resistance. Some of these metals are also present in distribution systems due to pipe corrosion and the presence of corrosion inhibitors. The goal of this research is to determine which water pipe materials and corrosion inhibitor choices result in minimal antibiotic resistance. This goal will be achieved through experiments to examine the role of corrosion on antibiotic resistance development. Collaborations between the research team and water utilities will facilitate research to determine the interplay between resistance genes, pipe surfaces, and drinking water chemistry in real-world distribution systems. Further benefits to society will result from guidance provided to drinking water utilities across the country. This guidance will help them decide which pipe materials and corrosion inhibitors best prevent the spread of antibiotic resistance in drinking water to protect public health. The spread of antibiotic resistance is a primary public health concern. Metals are a known stressor for antibiotic resistance and are prevalent in drinking water systems due to aging infrastructure and the use of metal-containing corrosion inhibitors. The central hypothesis of this work is that corrosion inhibitors and corrosion products yield metals in water distribution systems that increase the abundance and alter the types of antibiotic resistant genes in municipal drinking water. The research team combines expertise in chemistry and molecular microbiology to address three complimentary objectives. Objective 1 is to determine the impact of corrosion inhibitors on antibiotic resistance under variable nutrient conditions. Objective 2 is to elucidate the effect of corrosion products on antibiotic resistance using complimentary culture-based and non-culture-based techniques. Objective 3 is to identify linkages between corrosion products and the quantity and profile of antibiotic resistance genes in real-world drinking water distribution system pipes. Surface chemistry characterization will be coupled with Droplet Digital PCR to identify chemical relationships to antibiotic resistance gene profiles. Knowledge generated from this research will help utilities mitigate the spread of antibiotic resistance in drinking water distribution systems. Results will be incorporated into an annual two-day professional short course on emerging contaminants in water and wastewater. Additionally, the PIs will build on previous summer outreach efforts and host a high school course to engage underrepresented students in summer research to increase the diversity of the Nation’s STEM workforce.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

抗生素耐药性的传播通过使抗生素无效而威胁到公共卫生。饮用水分布系统是抗生素耐药性基因的来源，抗生素耐药性基因是使细菌抗抗生素具有抗生素的遗传物质。供水系统特别关注，因为它们是抗生素耐药性基因的直接输送机，包括儿童，老年人，医院患者以及免疫系统弱的人。已知金属促进抗生素耐药性的发展。由于管道腐蚀和腐蚀抑制剂的存在，这些金属中的一些也存在于分布系统中。这项研究的目的是确定哪种水管材料和腐蚀抑制剂的选择导致抗生素耐药性最小。该目标将通过实验来检查腐蚀在抗生素耐药性发展中的作用。研究团队与水电图之间的合作将有助于。研究以确定现实世界分布系统中抗性基因，管道表面和饮用水化学之间的相互作用。全国饮用水公用事业提供的指导将给社会带来更多的好处。该指南将帮助他们确定哪种管道材料和腐蚀抑制剂最能防止抗生素耐药性在饮用水中的传播以保护公共卫生。抗生素耐药性的传播是主要的公共健康问题。金属是抗生素耐药性的已知应激源，由于基础设施的老化和使用含金属的腐蚀抑制剂，在饮用水系统中普遍存在。这项工作的中心假设是，腐蚀抑制剂和腐蚀产物在水分配系统中产生金属，从而增加了市政饮用水中抗生素耐药基因的类型。研究小组结合了化学和分子微生物学方面的专业知识，以解决三个免费目标。目标1是确定腐蚀抑制剂对可变养分条件下抗生素耐药性的影响。目的2是使用基于基于培养的技术和非文化的技术来阐明腐蚀产物对抗生素耐药性的影响。目标3是确定腐蚀产物与现实饮用水分配系统管道中抗生素耐药基因的数量和概况之间的联系。表面化学表征将与液滴数字PCR结合，以鉴定与抗生素耐药性基因谱的化学关系。这项研究产生的知识将有助于公用事业减轻抗生素耐药性在饮用水分配系统中的传播。结果将纳入一年一度的为期两天的专业短期课程，以了解水和废水中的新兴污染物。此外，PI将在上夏季推广工作的基础上进行，并举办一门高中课程，使代表性不足的学生参与夏季研究，以增加美国STEM劳动力的多样性。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子优点和更广泛的影响审查标准来通过评估来获得的支持。