Collaborative Research: Mitigating antibiotic resistance in drinking water by understanding the impact of corrosion inhibitors and corrosion products

合作研究：通过了解腐蚀抑制剂和腐蚀产物的影响来减轻饮用水中的抗生素耐药性

• 批准号: 2027288
• 负责人: Patrick McNamara
• 金额: $ 21.63万
• 依托单位: Marquette University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027288&HistoricalAwards=false
• 关键词: Collaborative; Research; Mitigating; antibiotic; resistance

The spread of antibiotic resistance threatens public health by rendering antibiotics ineffective. Drinking water distribution systems are a source of antibiotic resistance genes, the genetic material that makes bacteria resistant to antibiotics. Water distribution systems are particularly concerning as they are a direct conveyor of antibiotic resistance genes to vulnerable populations including children, the elderly, hospital patients, and people with weakened immune systems. Metals are known to promote the development of antibiotic resistance. Some of these metals are also present in distribution systems due to pipe corrosion and the presence of corrosion inhibitors. The goal of this research is to determine which water pipe materials and corrosion inhibitor choices result in minimal antibiotic resistance. This goal will be achieved through experiments to examine the role of corrosion on antibiotic resistance development. Collaborations between the research team and water utilities will facilitate research to determine the interplay between resistance genes, pipe surfaces, and drinking water chemistry in real-world distribution systems. Further benefits to society will result from guidance provided to drinking water utilities across the country. This guidance will help them decide which pipe materials and corrosion inhibitors best prevent the spread of antibiotic resistance in drinking water to protect public health. The spread of antibiotic resistance is a primary public health concern. Metals are a known stressor for antibiotic resistance and are prevalent in drinking water systems due to aging infrastructure and the use of metal-containing corrosion inhibitors. The central hypothesis of this work is that corrosion inhibitors and corrosion products yield metals in water distribution systems that increase the abundance and alter the types of antibiotic resistant genes in municipal drinking water. The research team combines expertise in chemistry and molecular microbiology to address three complimentary objectives. Objective 1 is to determine the impact of corrosion inhibitors on antibiotic resistance under variable nutrient conditions. Objective 2 is to elucidate the effect of corrosion products on antibiotic resistance using complimentary culture-based and non-culture-based techniques. Objective 3 is to identify linkages between corrosion products and the quantity and profile of antibiotic resistance genes in real-world drinking water distribution system pipes. Surface chemistry characterization will be coupled with Droplet Digital PCR to identify chemical relationships to antibiotic resistance gene profiles. Knowledge generated from this research will help utilities mitigate the spread of antibiotic resistance in drinking water distribution systems. Results will be incorporated into an annual two-day professional short course on emerging contaminants in water and wastewater. Additionally, the PIs will build on previous summer outreach efforts and host a high school course to engage underrepresented students in summer research to increase the diversity of the Nation’s STEM workforce.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

抗生素耐药性的传播使抗生素失效，从而威胁到公共卫生。饮用水分配系统是抗生素耐药性基因的来源，这种遗传物质使细菌对抗生素产生耐药性。配水系统尤其令人担忧，因为它们是抗生素耐药性基因向弱势群体（包括儿童、老年人、医院病人和免疫系统较弱的人）的直接输送者。已知金属会促进抗生素耐药性的发展。由于管道腐蚀和腐蚀抑制剂的存在，其中一些金属也存在于分配系统中。这项研究的目的是确定哪些水管材料和腐蚀抑制剂的选择导致最小的抗生素耐药性。这一目标将通过实验来实现，以检查腐蚀对抗生素耐药性发展的作用。研究团队和水务公司之间的合作将促进研究，以确定现实世界分配系统中抗性基因，管道表面和饮用水化学之间的相互作用。向全国各地的饮用水公用事业提供指导，将给社会带来进一步的好处。该指南将帮助他们决定哪些管道材料和腐蚀抑制剂最能防止饮用水中抗生素耐药性的传播，以保护公众健康。抗生素耐药性的传播是一个主要的公共卫生问题。金属是抗生素耐药性的已知应激源，并且由于老化的基础设施和含金属的腐蚀抑制剂的使用，在饮用水系统中普遍存在。这项工作的中心假设是，腐蚀抑制剂和腐蚀产物在供水系统中产生金属，增加了城市饮用水中抗生素耐药基因的丰度并改变了其类型。 该研究团队结合了化学和分子微生物学的专业知识，以解决三个互补的目标。目的1是确定在不同营养条件下缓蚀剂对抗生素耐药性的影响。目的2是利用互补的基于培养和非基于培养的技术阐明腐蚀产物对抗生素耐药性的影响。目标3是确定腐蚀产物与真实世界饮用水分配系统管道中抗生素耐药基因的数量和分布之间的联系。表面化学表征将与Droplet Digital PCR相结合，以确定与抗生素耐药基因谱的化学关系。从这项研究中产生的知识将有助于公用事业减轻抗生素耐药性在饮用水分配系统中的传播。研究结果将纳入每年一次的为期两天的关于水和废水中新出现的污染物的专业短期课程。此外，PI将建立在以前的夏季外展工作，并举办高中课程，让代表性不足的学生参与夏季研究，以增加国家STEM劳动力的多样性。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Cast iron drinking water pipe biofilms support diverse microbial communities containing antibiotic resistance genes, metal resistance genes, and class 1 integrons

• DOI: 10.1039/d0ew01059f
• 发表时间: 2021-02
• 期刊: Environmental Science: Water Research & Technology