Collaborative Research: Elucidating the Diversity of Bacterial Flagellation and Motility Through Mechanics
合作研究:通过力学阐明细菌鞭毛和运动的多样性
基本信息
- 批准号:2027410
- 负责人:
- 金额:$ 31.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-01-01 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The ability of bacteria to swim crucial to survival. Swimming is made possible by rotating thin filaments called flagella. There are many different ways that flagella are arranged on bacteria, and many different movement patterns, all of which enable swimming. Despite the success of bacteria swimming, few of these mechanisms are well understood. The objective of this project is to determine how the arrangements and flexible mechanics of flagella combine to produce differences in bacterial motility. The results of this project will have numerous potential societal impacts, since motility enables bacteria to spread through water supplies, and to establish microbiomes or infections in humans. This work will have important effects at multiple levels. At the cellular level, better understanding bacterial motility will help us understand cell resource uptake. At the ecosystem scale, motility affects the food web. The results of this work could lead to new approaches to mediate bacterial infection, further our understanding of bacterial evolution, and inspire biomimetic microrobots with enhanced functionality. In addition, the award will support outreach programs that teach the public about bacterial locomotion and its importance to society through health and ecology.Bacterial flagellar motility has largely been relegated to two idealized paradigms for uni- and multiflagellated cells, respectively. However, mounting evidence suggests that bacteria exhibit a plethora of flagellar arrangements and associated motility patterns that do not fit into the existing paradigms. It is not understood how flagellar motility patterns emerge from variations in arrangements and the flexible mechanics of flagella. This work will develop a mechanistic understanding of the vast understudied diversity of flagellar arrangements and motility patterns by linking them to the geometry and mechanics of flagella. This biomechanical understanding has been hindered by a lack of quantitative imaging of flagella, limited ability to experimentally perturb flagellar properties, and overly simplified models. We will overcome these challenges by using unique imaging capabilities, which enable data-driven and experimentally validated numerical modeling and hypothesis testing. Experimentally, this work incorporates advanced high-speed video microscopy techniques to capture the time-resolved kinematics of 10-nanometer diameter flagella, pushing the envelope of existing quantification methods for fluid-structure interactions. Numerically, this work advances the state-of-the-art in modeling elastohydrodynamics of actuated filaments. This work will establish a unique data set comprising the mechanical properties of flagella and the kinematics of bacterial motility from diverse species. It will use modeling to determine the physical mechanisms leading to observed motility patterns, and combine physical and numerical experiments to elucidate and classify the originsThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
细菌的游泳能力对生存至关重要。游泳是通过旋转称为鞭毛的细丝来实现的。鞭毛在细菌上有许多不同的排列方式,以及许多不同的运动模式,所有这些都使游泳成为可能。尽管细菌游动取得了成功,但人们对这些机制知之甚少。这个项目的目标是确定鞭毛的排列和灵活的机制如何结合在一起,以产生细菌运动的差异。该项目的结果将产生许多潜在的社会影响,因为能动性使细菌能够通过供水传播,并在人类中建立微生物群或感染。这项工作将在多个层面产生重要影响。在细胞水平上,更好地了解细菌的运动性将有助于我们了解细胞资源的摄取。在生态系统尺度上,运动性影响食物网。这项工作的结果可能导致新的方法来中介细菌感染,进一步了解细菌的进化,并启发仿生微型机器人具有增强的功能。此外,该奖项将支持外展项目,通过健康和生态教育公众关于细菌移动及其对社会的重要性。细菌鞭毛移动在很大程度上已被归类为单鞭毛细胞和多鞭毛细胞的两种理想化范例。然而,越来越多的证据表明,细菌表现出过多的鞭毛排列和相关的运动模式,这不符合现有的范式。目前尚不清楚鞭毛运动模式是如何从鞭毛排列的变化和鞭毛的灵活机制中产生的。这项工作将通过将鞭毛排列和运动模式与鞭毛的几何和力学联系起来,从机制上理解鞭毛排列和运动模式的大量未被研究的多样性。缺乏鞭毛的定量成像、有限的实验扰乱鞭毛特性的能力以及过于简化的模型,阻碍了这种生物力学理解。我们将通过使用独特的成像功能来克服这些挑战,这些功能支持数据驱动和实验验证的数值建模和假设检验。在实验上,这项工作结合了先进的高速视频显微镜技术来捕捉直径10纳米的鞭毛的时间分辨运动学,突破了现有流体-结构相互作用的量化方法的界限。在数值上,这项工作推进了激励灯丝弹性流体动力学建模的最新水平。这项工作将建立一个独特的数据集,包括鞭毛的机械属性和来自不同物种的细菌运动学。它将使用建模来确定导致观察到的运动模式的物理机制,并结合物理和数值实验来阐明和分类来源。该奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Self-transport of swimming bacteria is impaired by porous microstructure
- DOI:10.1038/s42005-023-01136-w
- 发表时间:2023-01-24
- 期刊:
- 影响因子:5.5
- 作者:Dehkharghani, Amin;Waisbord, Nicolas;Guasto, Jeffrey S.
- 通讯作者:Guasto, Jeffrey S.
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Jeffrey Guasto其他文献
Jeffrey Guasto的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Jeffrey Guasto', 18)}}的其他基金
Collaborative Research: Stability and dispersion of viscoelastic flows through porous media
合作研究:多孔介质粘弹性流的稳定性和分散性
- 批准号:
2141349 - 财政年份:2022
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
Collaborative Research: Viral induced chemotaxis mediating cross-trophic microbial interactions and carbon flux
合作研究:病毒诱导的趋化性介导跨营养微生物相互作用和碳通量
- 批准号:
1829827 - 财政年份:2018
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
Collaborative Research: Effects of Fluid Flow on Flagellar Mechanics and Microbial Motility
合作研究:流体流动对鞭毛力学和微生物运动的影响
- 批准号:
1701392 - 财政年份:2017
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
CAREER: The mechanics and control of cell dispersal
职业:细胞分散的机制和控制
- 批准号:
1554095 - 财政年份:2016
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
UNS:Collaborative Research: Transport and Chemotaxis of Swimming Cells in Porous Media Flows
UNS:合作研究:多孔介质流中游动细胞的运输和趋化性
- 批准号:
1511340 - 财政年份:2015
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
相似国自然基金
Research on Quantum Field Theory without a Lagrangian Description
- 批准号:24ZR1403900
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
Cell Research
- 批准号:31224802
- 批准年份:2012
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research
- 批准号:31024804
- 批准年份:2010
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research (细胞研究)
- 批准号:30824808
- 批准年份:2008
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Research on the Rapid Growth Mechanism of KDP Crystal
- 批准号:10774081
- 批准年份:2007
- 资助金额:45.0 万元
- 项目类别:面上项目
相似海外基金
Collaborative Research: Elucidating High Temperature Deformation Mechanisms in Refractory Multi-Principal-Element Alloys
合作研究:阐明难熔多主元合金的高温变形机制
- 批准号:
2313860 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
Collaborative Research: RUI: Elucidating Design Rules for non-NRPS Incorporation of Amino Acids on Polyketide Scaffolds
合作研究:RUI:阐明聚酮化合物支架上非 NRPS 氨基酸掺入的设计规则
- 批准号:
2300890 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Continuing Grant
Collaborative Research: RUI: Elucidating Design Rules for non-NRPS Incorporation of Amino Acids on Polyketide Scaffolds
合作研究:RUI:阐明聚酮化合物支架上非 NRPS 氨基酸掺入的设计规则
- 批准号:
2300891 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Continuing Grant
Collaborative Research: MRA: Elucidating the multi-dimensionality and scaling of avian diversity-vegetation relationships
合作研究:MRA:阐明鸟类多样性与植被关系的多维性和尺度
- 批准号:
2307188 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
Collaborative Research: Elucidating the Role of Natural Aerosols in Modulating Boundary Layer Clouds and Precipitation in the Southern Ocean
合作研究:阐明天然气溶胶在调节南大洋边界层云和降水中的作用
- 批准号:
2246489 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Continuing Grant
Collaborative Research: Elucidating High Temperature Deformation Mechanisms in Refractory Multi-Principal-Element Alloys
合作研究:阐明难熔多主元合金的高温变形机制
- 批准号:
2313861 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
Collaborative Research: Elucidating the roles of biogenic exudates in the cycling and uptake of rare earth elements
合作研究:阐明生物渗出物在稀土元素循环和吸收中的作用
- 批准号:
2221913 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
Collaborative Research: MRA: Elucidating the multi-dimensionality and scaling of avian diversity-vegetation relationships
合作研究:MRA:阐明鸟类多样性与植被关系的多维性和尺度
- 批准号:
2307189 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
Collaborative Research: Elucidating the Ocean Dynamics Governing Melt at Glaciers Using Lagrangian Floats
合作研究:利用拉格朗日浮标阐明控制冰川融化的海洋动力学
- 批准号:
2319494 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant
Collaborative Research: Elucidating Exciton Transport in Hierarchical Organic Materials through Time-Resolved Electronic and Vibrational Spectroscopy/Microscopy
合作研究:通过时间分辨电子和振动光谱/显微镜阐明多级有机材料中的激子传输
- 批准号:
2401851 - 财政年份:2023
- 资助金额:
$ 31.25万 - 项目类别:
Standard Grant