CAREER: The mechanics and control of cell dispersal

职业：细胞分散的机制和控制

• 批准号: 1554095
• 负责人: Jeffrey Guasto
• 金额: $ 50.41万
• 依托单位: Tufts University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1554095&HistoricalAwards=false
• 关键词: CAREER; mechanics; control; cell; dispersal

PI: Guasto, Jeffrey Proposal Number: 1554095 This CAREER proposal is focused on the investigation of how the material properties of viscous fluids affect the motion of cells. The goal is to contribute insights into the broad field of cell transport, and to understand how different physical processes affecting the swimming of cells can regulate a host of diverse biological functions. The results of this research could have an impact on multiple fields, including public health, ecology, engineering and medicine. Phenomena that are important to the treatment of fertility and the mechanisms of spreading of disease will be investigated.While most prior work in this area has been focused on cases of swimming in Newtonian fluids (aquatic environments mainly), and ideally shaped swimmers, this is a proposal to investigate the swimming of real bacteria cells and in non-Newtonian fluids, like cervical mucus. The development of a novel nano-scale experimental technique, based on high speed imaging, for the monitoring of the motion of each individual cell is also proposed. In addition to graduate and undergraduate student training, a new outreach program (STOMP) will be developed in collaboration with Tufts Center for Engineering Education and Outreach that will be based on using high-speed imaging.

主要研究者：Guasto，Jeffrey Proposal Number：1554095这份职业建议书的重点是研究粘性流体的材料特性如何影响细胞的运动。其目标是对细胞运输的广泛领域提供见解，并了解影响细胞游泳的不同物理过程如何调节多种生物功能。这项研究的结果可能会对多个领域产生影响，包括公共卫生，生态学，工程和医学。研究对生育治疗和疾病传播机制有重要意义的现象。虽然该领域的大多数先前工作都集中在牛顿流体（主要是水生环境）中游泳的情况下，以及理想形状的游泳者，但这是一个研究真实的细菌细胞和非牛顿流体（如宫颈粘液）中游泳的建议。还提议开发一种基于高速成像的新型纳米级实验技术，用于监测每个单个细胞的运动。 除了研究生和本科生培训，一个新的推广计划（STOMP）将与塔夫茨工程教育和推广中心合作开发，将基于使用高速成像。

项目成果

Bacterial chemotaxis in a microfluidic T-maze reveals strong phenotypic heterogeneity in chemotactic sensitivity

• DOI: 10.1038/s41467-019-09521-2
• 发表时间: 2019-04-23
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Salek, M. Mehdi;Carrara, Francesco;Stocker, Roman
• 通讯作者: Stocker, Roman

Flagellar kinematics reveals the role of environment in shaping sperm motility

• DOI: 10.1098/rsif.2020.0525
• 发表时间: 2020-09-30
• 期刊: JOURNAL OF THE ROYAL SOCIETY INTERFACE
• 影响因子: 3.9
• 作者: Guasto, Jeffrey S.;Estrada, Jonathan B.;Stocker, Roman
• 通讯作者: Stocker, Roman

Perspectives on viscoelastic flow instabilities and elastic turbulence

• DOI: 10.1103/physrevfluids.7.080701
• 发表时间: 2022-08-29
• 期刊: PHYSICAL REVIEW FLUIDS
• 影响因子: 2.7
• 作者: Datta, Sujit S.;Ardekani, Arezoo M.;Stone, Howard A.
• 通讯作者: Stone, Howard A.

Dynamic mode structure of active turbulence

主动湍流动力模态结构

• DOI: 10.1103/physrevfluids.8.023101
• 发表时间: 2023
• 期刊: Physical Review Fluids
• 影响因子: 2.7
• 作者: Henshaw, Richard J.;Martin, Olivia G.;Guasto, Jeffrey S.
• 通讯作者: Guasto, Jeffrey S.

Microfluidic quantification and separation of yeast based on surface adhesion

基于表面粘附的酵母微流控定量和分离

• DOI: 10.1039/c9lc00275h
• 发表时间: 2019
• 期刊: Lab on a Chip
• 影响因子: 6.1
• 作者: Reinmets, Kristina;Dehkharghani, Amin;Guasto, Jeffrey S.;Fuchs, Stephen M.
• 通讯作者: Fuchs, Stephen M.