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The roles of electrically driven water flow on cellular viability, tissue repair and polarity.

电驱动水流对细胞活力、组织修复和极性的作用。

基本信息

批准号：
2033522
负责人：
Mark Messerli
金额：
$ 46.5万
依托单位：
South Dakota State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-05-15 至 2025-04-30
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2033522&HistoricalAwards=false
关键词：
roles electrically driven water flow

项目摘要

This project will investigate how electricity generates force to move water through the tiniest spaces in the human body. To support human life, water flow commonly occurs through thousands of miles of blood vessels to provide cells with nutrients and remove their waste products. In the absence of blood flow, cells suffocate or starve. Electrically driven water flow between cells provides significant advantages over pressure driven flow for promoting cell survival in the absence of blood flow. This approach will be useful for promoting cell survival during tissue engineering prior to building of a new blood vessel network. This project may also reveal how electricity promotes healing of degenerative diseases caused by damaged or absent blood vessels. As part of the educational broader impacts of this project, investigators will provide cross-disciplinary training for student researchers and provide professional development workshops for secondary-school science teachers to enhance their ability to integrate tissue engineering modules into their classrooms.Soft tissue repair is often plagued by poor or absent blood flow, and interstitial flow in the extracellular matrix and around cells is lost. This project will characterize the effects of interstitial flow on cellular viability and proliferation using fluorescent assays, when interstitial flow is generated by electricity or pressure. In addition, mechanosensitive pathways used by cells to sense electrically driven water flow will be characterized using a combination of real-time fluorescence imaging and protein manipulation. Finally, applied electric fields are well known to polarize cells in 2D culture but their usefulness in 3D culture is unknown. Electrically driven water flow will be tested to polarize cells in 3D culture, in order to help direct growth of new tissues required for repair of damaged tissues. Results from this work will be influential for guiding current medical interventions for soft tissue repair during physical therapies, surgical methods, and survival of tissues and organs during advanced tissue engineering. The science and engineering supporting this research will also be used to promote tissue engineering modules for students in secondary education through a ‘teach the teachers’ training workshop.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将研究电力如何产生力量，使水通过人体最微小的空间。为了支持人类的生命，水流通常通过数千英里的血管为细胞提供营养并清除它们的废物。在没有血液流动的情况下，细胞会窒息或饥饿。细胞之间的电驱动水流提供了优于压力驱动流的显著优点，用于在没有血流的情况下促进细胞存活。这种方法将有助于在构建新血管网络之前促进组织工程期间的细胞存活。该项目还可能揭示电力如何促进由受损或缺失的血管引起的退行性疾病的愈合。作为该项目的教育影响的一部分，研究人员将为学生研究人员提供跨学科培训，并为中学科学教师提供专业发展研讨会，以提高他们将组织工程模块整合到课堂中的能力。软组织修复通常受到血液流动不良或缺乏的困扰，细胞外基质和细胞周围的间质流动丢失。该项目将使用荧光分析来表征当间质流由电或压力产生时，间质流对细胞活力和增殖的影响。此外，细胞用于感知电驱动水流的机械敏感途径将使用实时荧光成像和蛋白质操纵的组合来表征。最后，所施加的电场在2D培养中对细胞的增殖是众所周知的，但它们在3D培养中的有用性是未知的。将测试电驱动水流以在3D培养中培养细胞，以帮助修复受损组织所需的新组织的直接生长。从这项工作的结果将是有影响力的物理治疗，手术方法和先进的组织工程过程中的组织和器官的生存软组织修复的指导目前的医疗干预。支持这项研究的科学和工程也将用于通过“教教师”培训讲习班为中等教育的学生推广组织工程模块。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。